LOCUS AK299016 3053 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ57848 complete cds, highly similar to Homo
sapiens hydrocephalus inducing (HYDIN), mRNA.
ACCESSION AK299016
VERSION AK299016.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3053)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3053
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2026046"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493640"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 23..2845
/codon_start=1
/note="highly similar to Homo sapiens hydrocephalus
inducing (HYDIN), mRNA"
/protein_id="BAG61096.1"
/transl_table=1
/translation="MESAGGFKLGEKKKLLKMTSRRLEESMGAVQMGLVNMFKGFQSK
VLPPLSPKVVTEEEVNRMLTPSEFLKEMSLTTEQRLAKTRLMCRPQIIELLDMGETTH
QKFSGIDLDQALFQPFPSEIIFQNYTPCEVYEVPLILRNNDKIPRLVKVVEESSPYFK
VISPKDIGHKVAPGVPSIFRILFTPEENKDYAHTLTCVTEREKFIVPIKARGARAILD
FPDKLNFSTCPVKYSTQKILLVRNIGNKNAVFHIKTCRPFSIEPAIGTLNVGESMQLE
VEFEPQSVGDHSGRLIVCYDTGEKVFVSLYGAAIDMNIRLDKNSLTIEKTYISLANQR
TITIHNRSNIIAHFLWKVFATQQEEDREKYRACDDLIKEEKDETDEFFEECITDPLLR
EHLSVLSRTFANQRRLVQGDSKLFFNNVFTVEPLEGDVWPNSSAEITVYFNPLEAKLY
QQTIYCDILGREIRLPLRIKGEGMGPKIHFNFELLDIGKVFTGSAHCYEAILYNKGSI
DALFNMTPPTSALGACFVFSPKEGIIEPSGVQAIQISFSSTILGNFEEEFLVNVNGSP
EPVKLTIRGCVIGPTFHFNVPALHFGDVSFGFPHILICSLNNTSLIPMTYKLRIPGDG
LGHKSISYCEQHVDYKRPSWTKEEISSMKPKEFTISPDCGTIRPQGFAAIRVTLCSNT
VQKYELALVVDVEGIGEEVLALLITARCVVPALHLVNTEVDFGHCFLKYPYEKTLQLA
NQDDLPGFYEVQPQVCEEVPTVLFSSPTPSGVISPSSTIHIPLVLETQVTGEHRSTVY
ISIFGSQDPPLVCHLKSAGEGPVIYVHPNQVDFGNIYVLKDSSRILNLCNQSFIPAFF
QAHMAHKKSLWTIEPNEGMVPPETDVQLALTANLNDTLTFKNCVILDIENSSTYRIPV
QASGTGSTIVSDKPFAPELNLGAHFR"
BASE COUNT 853 a 731 c 671 g 798 t
ORIGIN
1 acggcaacag ctcgggcggc gcatggagag tgcgggcggc ttcaagctgg gtgagaaaaa
61 aaaattacta aaaatgacaa gtagaagact tgaggagtcc atgggggctg ttcagatggg
121 attggtcaat atgttcaaag gatttcaaag caaggttttg ccacccctga gtccaaaggt
181 ggttacagaa gaagaagtaa accgaatgct tacaccctca gagttcctga aggaaatgtc
241 cctgaccacc gagcagagac tggcaaaaac acgtttgatg tgccgaccac agatcatcga
301 actcttagat atgggggaaa caacacatca gaagttttca ggaattgacc tggatcaggc
361 attattccag ccctttccat cagaaattat atttcagaac tacactccct gtgaagtcta
421 tgaagttcca ctgattttga ggaacaatga caaaattcca aggttggtga aagttgtgga
481 agaaagttcg ccttacttta aagtaatcag ccccaaagat attggccaca aagtggctcc
541 tggagtgcct tccatattcc gaatcctctt tactccagag gagaacaagg attacgccca
601 tacgttgacc tgtgttactg aaagagaaaa gtttattgta cccatcaaag ctagaggggc
661 acgagccatt ctcgattttc ctgacaagct gaatttttcc acttgtcctg tcaaatacag
721 cacccagaag attctgctgg tacgaaacat tggcaacaaa aatgctgtat ttcacatcaa
781 aacttgtagg cctttctcta tagaaccagc tattggaact cttaatgtgg gagagtccat
841 gcaactggaa gtggagtttg agccacagag tgtgggcgat cacagtggaa gacttatcgt
901 gtgttatgac acaggtgaaa aggtgtttgt atctctctat ggagctgcca tagacatgaa
961 tataaggctg gataagaatt ccttgaccat cgagaaaacc tacatatctc tggccaatca
1021 gcgaactata accattcaca atcgcagtaa tatcattgcc catttcctgt ggaaggtatt
1081 tgctacccag caagaagagg acagagaaaa atatagggcc tgtgatgatc tgatcaaaga
1141 ggagaaggat gagactgatg agttttttga agagtgcatt actgatcctt tactccgaga
1201 acatctttct gttctgtccc gaacctttgc gaatcagagg aggctggtgc agggagacag
1261 caagctgttc ttcaataacg ttttcactgt ggagcccctg gaaggtgatg tctggcccaa
1321 ctcatcagct gaaatcaccg tgtactttaa cccactagaa gccaagctct atcaacagac
1381 catttactgc gacattttag gccgagaaat ccgtctgccc ctccgaatca aaggggaagg
1441 catgggacct aagattcact tcaactttga attgctggat attgggaaag ttttcactgg
1501 atctgcacat tgttatgagg cgatactgta caacaaaggc agcatcgatg ctctcttcaa
1561 catgacccct ccaacttcag ctttgggggc ctgctttgtt ttcagtccca aggaaggcat
1621 cattgaacca agtggagtcc aagctatcca gatctccttc agctctacca tcctgggaaa
1681 ctttgaagaa gagttcctgg tcaatgtcaa tgggtcacct gagcctgtga aactgaccat
1741 tagaggctgt gtcattggac ctaccttcca ttttaatgtt ccagctctgc actttggtga
1801 tgtttccttt gggtttcctc atatcttgat atgttccctc aataatacct ctttgatccc
1861 catgacttac aaactgcgta tccctgggga tggccttggc cataaaagca tttcatattg
1921 tgagcagcat gtggactaca aaagaccatc ttggaccaag gaagaaatat cctcaatgaa
1981 accaaaagaa ttcaccatct ctcctgactg tggcaccatt cgcccccagg gatttgctgc
2041 tatcagggtg acattatgct ccaacactgt gcagaaatac gagctggcac tcgtggtgga
2101 cgtggagggc atcggagaag aggtgctggc gctcttaatt acagcaaggt gtgttgtacc
2161 tgccctccac ctggtcaata cagaggtgga ctttgggcac tgcttcctga agtacccgta
2221 tgagaaaaca ctccagcttg ccaatcaaga tgacctccca ggattctatg aggtccagcc
2281 tcaggtgtgt gaggaggtgc ctactgtgct gttttccagc cccaccccca gcggggtcat
2341 ctccccaagc agcaccatcc acataccact ggtcctggag acccaggtca ctggagaaca
2401 cagatccacg gtttacatct caatctttgg gagccaggac ccccctttgg tatgtcactt
2461 aaagagcgct ggagaaggcc cagttatcta cgtccatccc aatcaagtgg acttcgggaa
2521 tatctacgtc ctaaaagact cttccaggat tctcaaccta tgcaaccagt ccttcattcc
2581 cgcatttttc caggcacaca tggcacacaa aaaatccctt tggacgattg aacccaatga
2641 aggcatggtt cctccagaaa ctgatgttca actggcactg accgccaacc tgaatgacac
2701 actgacattc aagaactgtg ttattttgga cattgaaaat agcagtacct atcggattcc
2761 tgttcaggct tccggaactg gttccactat tgtttcagat aagccctttg ctccagaact
2821 caatttgggg gcacatttta ggtaaggaaa tctcatggac cagtttttat agatatcaca
2881 tatgttacat atgctgagca tcacctctac tttagaaaat tattttaagt aattaaaact
2941 agccacctca gtccctctcc tttcccatcc tcactcttcc acctcctgat gtgtatgtca
3001 gcaacaagaa gacattttct agccataact tggttgcatt ctctaatccg tgt
//