LOCUS AK299011 2150 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58106 complete cds, highly similar to
Nephrocystin-1.
ACCESSION AK299011
VERSION AK299011.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2150)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2150
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2025387"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493635"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 24..2054
/codon_start=1
/note="highly similar to Nephrocystin-1"
/protein_id="BAG61092.1"
/transl_table=1
/translation="MLARRQRDPLQALRRRNQELKQQVDSLLSESQLKEALEPNKRQH
IYQRCIQLKQAIDENKNALQKLSKADESAPVANYNQRKEEEHTLLDKLTQQLQGLAVT
ISRENITEVGAPTEEEEESESEDSEDSGGEEEDAEEEEEEKEENESHKWSTGEEYIAV
GDFTAQQVGDLTFKKGEILLVIEKKPDGWWIAKDAKGNEGLVPRTYLEPYSEEEEGQE
SSEEGSEEDVEAVDETADGAEVKQRTDPHWSAVQKAISEINTVDVLTTMGAIPAGFRP
STLSQLLEEGNQFRANYFLQPELMPSQLAFRDLMWDATEGTIRSRPSRISLILTLWSC
KMIPLPGMSIQVLSRHVRLCLFDGNKVLSNIHTVRATWQPKKPKTWTFSPQVTRILPC
LLDGDCFIRSNSASPDLGILFELGISYIRNSTGERGELSCGWVFLKLFDASGVPIPAK
TYELFLNGGTPYEKGIEVDPSISRRAHGSVFYQIMTMRRQPQLLVKLRSLNRRSRNVL
SLLPETLIGNMCSIHLLIFYRQILGDVLLKDRMSLQSTDLISHPMLATFPMLLEQPDV
MDALRSSWAGKESTLKRSEKRDKEFLKSTFLLVYHDCVLPLLHSTRLPPFRWAEEETE
TARWKVITDFLKQNQENQGALQALLSPDGVHEPFDLSEQTYDFLGEMRKNAV"
BASE COUNT 681 a 434 c 510 g 525 t
ORIGIN
1 atcagagcac cgcagccagg gagatgctgg cgagacgaca gcgagatcct ctccaggccc
61 tgcggcgccg caatcaggag ctgaagcaac aggttgatag tttgctttct gagagccaac
121 tgaaagaagc tctagaaccc aataaaagac aacatattta tcaaagatgt atccagttaa
181 agcaggcaat agatgaaaat aaaaatgctc ttcaaaaatt aagcaaagct gatgaatctg
241 cacctgttgc aaactataat cagagaaaag aagaggagca tactcttttg gacaagctta
301 cccaacaact gcagggcctt gctgtgacaa taagcagaga aaatataact gaagttgggg
361 cacctactga agaagaggaa gaaagtgaaa gtgaagatag tgaagacagt ggtggggagg
421 aagaagatgc agaggaggaa gaggaagaga aagaggaaaa tgaatctcac aaatggtcaa
481 ccggtgaaga atacatcgct gttggagatt ttactgctca gcaagttgga gatcttacat
541 ttaagaaagg ggaaattctc cttgtaattg aaaaaaaacc tgatggttgg tggatagcta
601 aggatgccaa aggaaatgaa ggtcttgttc ccagaaccta cctagagcct tatagtgaag
661 aagaagaagg ccaagaatca agtgaagagg gcagtgaaga agatgtagag gcggtggatg
721 aaacagcaga tggagcagaa gttaagcaaa gaactgatcc ccactggagt gctgttcaga
781 aagcgatttc agagataaac actgttgatg tgttaactac gatgggagct attcctgcag
841 ggttcaggcc ttccacgctc tcacagcttc tggaggaagg gaatcaattt cgagcaaatt
901 acttcttaca accagagctc atgccttcac aactggcctt cagagatctg atgtgggatg
961 ctacagaagg cactattagg tcgagaccaa gtcgtatttc attgattctg acattatgga
1021 gctgtaaaat gattcctctt ccaggaatga gcatacaggt tctcagcaga catgtacgcc
1081 tctgtctatt tgatggtaat aaggttctga gcaacattca tacagtcaga gccacatggc
1141 aacctaaaaa gcccaaaaca tggacctttt ctccccaggt tactcgcatc ttaccatgtt
1201 tgcttgatgg tgattgcttt atcaggtcta attctgcatc tccagatctt ggaatattat
1261 ttgaacttgg aatttcttat attcgcaatt caactggtga aagaggagag ttaagctgtg
1321 gctgggtgtt tcttaaactt tttgatgcca gtggagttcc tattccagca aaaacttatg
1381 agcttttctt gaatggtggt actccttatg aaaaaggtat tgaagtggac ccttcaatat
1441 ccagaagagc acacggcagt gttttctacc agattatgac aatgagaagg cagcctcaac
1501 ttctagtgaa actgagatcc ttgaacagaa gatcaagaaa tgtactaagt ctactgccag
1561 aaacattaat tggaaatatg tgttctattc acttgttgat attttatcga caaattcttg
1621 gagatgtgct cctgaaagac aggatgagct tgcaaagtac tgatttaatt agccatccca
1681 tgctggccac cttccccatg ctcttggagc agcctgatgt gatggatgct ctcaggagtt
1741 cgtgggctgg aaaagaaagc acattaaaaa gatcagagaa gagagacaaa gagttcctga
1801 agtccacgtt tctcctggtt taccatgact gcgtgctccc acttctccac tccacacgcc
1861 tacccccatt caggtgggca gaagaagaga ctgagactgc acggtggaaa gttatcactg
1921 acttccttaa gcaaaaccaa gaaaaccagg gcgccctcca agctctgctg tcaccagacg
1981 gagttcatga accttttgac ctttcagagc agacctatga cttcttgggt gaaatgagaa
2041 agaatgcagt gtgacagtgg cagcctctag ccctcagctt cccacggaat cagatggatc
2101 ctccacgatt acgtgaataa aatgatggaa ccaaaaatca ctgtcacttt
//