LOCUS AK299010 914 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ59962 complete cds, moderately similar to
Microtubule-actin crosslinking factor 1(Actin cross-linking family
7).
ACCESSION AK299010
VERSION AK299010.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 914)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..914
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2025159"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493634"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 180..545
/codon_start=1
/note="moderately similar to Microtubule-actin
crosslinking factor 1(Actin cross-linking family 7)"
/protein_id="BAG61091.1"
/transl_table=1
/translation="MSSSDEETLSERSCRSERSCRSERSYRSERSGSLSPCPPGDTLP
WNLPLHEQKKRKSQDSVLDPAERAVVRVADALPSTSFCFPHCSLLRNAAAVSPIRSKR
KSKTKRRKPQSFCDCVVWL"
BASE COUNT 198 a 284 c 210 g 222 t
ORIGIN
1 attgtgggag ccgctcccct cggctccgcc acgctcccct cgactgcgct ccagcctggg
61 gcgcgcccgg ccgccgccgc cttcgctgcc gccacgggcc cgtcttcttc ctccttcggc
121 tcccaggatc acttctccct gggctcccag gccctcctgc agcagccccc gcctgggcca
181 tgtcttcctc agatgaagag acgctcagtg agcggtcatg tcggagtgag cggtcttgtc
241 ggagtgagcg atcttacagg agcgagcggt cggggagcct gtctccctgt cccccagggg
301 acaccttgcc ctggaacctg ccactgcatg agcagaaaaa gcggaaaagc caggattcgg
361 tgctggaccc tgcagagcgt gctgtggtca gagtcgctga tgcccttccg agtacatcct
421 tctgcttccc ccattgcagc cttcttagaa atgcagcagc agtctcaccc atcaggagca
481 aaagaaaatc aaagaccaaa aggagaaagc ctcagagctt ttgcgactgt gtggtctggc
541 tataaattta gactgttgct tgaaagaagc gaaaaacctc cccttcatag acttccttct
601 tcccatcccc cctggcagcc gctttgttcc tctagccttg taaaattttc tctgtataaa
661 acaggaacat cagccaaaca tgtggacttc cccactttcc attcattaga agaatgcatt
721 atttcaaaat ctcctgggag catttaaatg caaattttgt caagaaagca atttgagtct
781 tagagaagcc ctctagccac aacctaaagc agaagtttct tctttgattt gtgtgtttct
841 taaaaggaat ctcaacccct tgccttctcc ctgcaacgcc cccaggtctt ctctgattct
901 cacagcggac tctt
//