LOCUS AK299003 2359 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ56927 complete cds, highly similar to
Centrosomal protein of 70 kDa.
ACCESSION AK299003
VERSION AK299003.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2359)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2359
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2023637"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493627"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 146..1879
/codon_start=1
/note="highly similar to Centrosomal protein of 70 kDa"
/protein_id="BAH12924.1"
/transl_table=1
/translation="MTEQEEAEWESINVLLMMHGLKPLSLVKRTDLKDLIIFDKQSSQ
RMRQNLKLLVEETSCQQNMIQELIETNQQLRNELQLEQSRAANQEQRANDLEQIMESV
KSKIGELEDESLNRACHQQNKIKDLQKEQKTLQVKCQHYKKKRTEQEETIASLQMEVC
RLKKEEEDRIVTQNRVFAYLCKRVPHTVLDRQLLCLIDYYESKIRKIHTQRQYKEDES
QSEEENDYRNLDASPTYKGLLMSLQNQLKESKSKIDALSSEKLNLQKDLETRPTLHEL
RLYKQQVKKLEKALKKNVKLQELINHKKAEDTEKKDEPSKYNQQQALIDQRYFQVLCS
INSIIHNPRAPVIIYKQTKGGVQNFNKDLVQDCGFEHLVPVIEMWADQLTSLKDLYKS
LKTLSAELVPWLNLKKQDENEGIKVEDLLFIVDTMLEEVENKEKDSNMPHFQTLQAIV
SHFQKLFDVPSLNGVYPRMNEVYTRLGEMNNAVRNLQELLELDSSSSLCVLVSTVGKL
CRLINEDVNEQVMQVLGPEDLQSIIYKLEEHEEFFPAFQAFTNDLLEILEIDDLDAIV
PAVKKLKVLSY"
BASE COUNT 835 a 387 c 489 g 648 t
ORIGIN
1 agcctaacct ggcccggctg gctgctggtg ggaggccggc gactgagtaa gatgaagatc
61 cagtttcaga catgagactc gttgggtgat catttcattg agatcaacct gaatgaccag
121 gtgtaaagtg caagagtaat atgctatgac tgagcaggaa gaagcagaat gggaaagcat
181 aaatgtgcta ttgatgatgc atggcttaaa acctttgtct ctagtcaaaa gaacagatct
241 caaagatctc atcatttttg acaaacagtc atcacaaagg atgagacaga atttgaaatt
301 gttggtggaa gaaacatcat gtcaacagaa catgatacag gagcttatag aaactaatca
361 acagcttaga aatgaacttc agctagagca aagccgagca gccaatcaag aacaacgagc
421 taatgacttg gaacaaatta tggaaagtgt gaaatccaaa attggtgaat tggaggatga
481 atcactaaat agggcttgcc accaacagaa taaaataaaa gatcttcaaa aggagcagaa
541 aactttgcag gtgaagtgcc agcattataa gaaaaaacga acggagcaag aagaaactat
601 tgcttctttg caaatggaag tctgtagatt aaaaaaggag gaagaagatc gcattgtcac
661 tcaaaacaga gtgtttgcct atctgtgcaa aagagttcct cataccgtct tggatagaca
721 gttgctttgt ctaattgatt actatgaatc taaaattaga aaaattcata cacaaaggca
781 atataaagaa gatgaaagtc agtcagaaga agaaaacgac tacagaaatc tggatgcctc
841 accaacttat aaaggccttt taatgtcatt acagaatcaa ctcaaggaat caaaatctaa
901 gattgatgca ctttcaagtg aaaagctgaa cctccaaaaa gatttggaaa ccaggcctac
961 gctgcatgaa ttaagacttt ataaacagca ggtgaagaag ctggaaaaag cccttaagaa
1021 aaacgtcaaa ttacaggagc ttattaatca taagaaggct gaggacacag agaagaaaga
1081 tgagcccagc aaatataatc agcaacaggc cctaattgac cagagatact ttcaggtgct
1141 gtgtagcatc aattcaatta tccacaatcc aagagctcca gtaataattt ataaacagac
1201 caaaggggga gtccaaaatt ttaataaaga tcttgttcaa gattgtggat ttgagcatct
1261 tgttcctgta atagaaatgt gggcagatca actgacatcc ttaaaggatt tgtataagtc
1321 cttgaaaaca ctatctgcag aactggtacc ttggcttaat ttgaagaagc aggatgaaaa
1381 tgaaggtatc aaagttgaag atttgttgtt tatagtagat actatgctgg aagaagttga
1441 aaataaggaa aaggacagca atatgccaca ctttcaaact ttgcaagcta ttgtttctca
1501 cttccaaaag ttatttgatg tgccttcttt aaatggagtc tatccccgaa tgaatgaagt
1561 ttatactagg cttggagaaa tgaacaatgc tgtgagaaac ctccaagaac tcttagaatt
1621 agatagttca tcctcattgt gtgtgctagt aagcactgtt ggaaaactct gtaggctgat
1681 taatgaagat gtgaatgagc aggttatgca ggtattagga cctgaagacc tccagagcat
1741 tatctacaaa ttggaagaac acgaggaatt tttcccagca tttcaggcat ttactaatga
1801 tctacttgaa atcttagaaa ttgatgactt ggatgccatt gtacctgcag taaagaaatt
1861 aaaagtactt tcatactgaa aacaaatcaa atcattttta ctgtgtaaat tgtattctta
1921 acattttgta ttttgtagga ttgatcttat tttgagacaa gggttgtaaa atgtatttgc
1981 tctcagaatt catccccttc ttagtattag gtcattcttc atctgtgtaa tttttattaa
2041 ctttgagatt cttatacaga aaatagttgc aagggtaagg taattaactt tagatccctt
2101 catttctaaa gcaattattg agtgcttaaa atttaaggtt gttttagagt ggagtttgtg
2161 agtaaaagag gagcccaggg agacagattg caagtagtag gttgcaatct tttagattca
2221 gcttgtaata atctttgagc cacaccaaaa ggaatgccaa gggctctcta aatttagcca
2281 gtaggaagct actgctctcc tggcaaggat taattggcac attctgttaa gatatccctc
2341 atgacagttg ccctttcaa
//