LOCUS AK298985 1755 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ53291 complete cds, highly similar to
Beta-galactosidase-related protein precursor.
ACCESSION AK298985
VERSION AK298985.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1755)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1755
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2020093"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493609"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 58..1698
/codon_start=1
/note="highly similar to Beta-galactosidase-related
protein precursor"
/protein_id="BAH12915.1"
/transl_table=1
/translation="MPGFLVRILLLLLVLLLLGPTRGLRNATQRMFEIDYSRDSFLKD
GQPFRYISGSIHYSRVPRFYWKDRLLKMKMAGLNAIQTLPGSCGQVVGSPSAQDEASP
LSEWRASYNSAGSNITDAFLSQRKCEPKGPLINSEFYTGWLDHWGQPHSTIKTEAVAS
SLYDILARGASVNLYMFIGGTNFAYWNGANSPYAAQPTSYDYDAPLSEAGDLTEKYFA
LRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDILCPSGPIKSLYPLTFI
QVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGIPQGVLERNNVITLNI
TGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTDWTIFPLDTEDAVRSH
LGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLPQDTFIQFPGWTKGQV
WINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAPCSSDDPELCAVTFV
DRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV"
BASE COUNT 415 a 488 c 440 g 412 t
ORIGIN
1 aagtgacgcg aagcggccgg cctgggcgcc gactgcagag ccgggaggct ggtggtcatg
61 ccggggttcc tggttcgcat cctccttctg ctgctggttc tgctgcttct gggccctacg
121 cgcggcttgc gcaatgccac ccagaggatg tttgaaattg actatagccg ggactccttc
181 ctcaaggatg gccagccatt tcgctacatc tcaggaagca ttcactactc ccgtgtgccc
241 cgcttctact ggaaggaccg gctgctgaag atgaagatgg ctgggctgaa cgccatccag
301 acattacctg gcagctgtgg acaagtggtt gggagtcctt ctgcccaaga tgaagcctct
361 cctctatcag aatggagggc cagttataac agtgcaggca gcaacatcac agatgctttc
421 ctaagccaga ggaagtgtga gcccaaagga cccttgatca attctgaatt ctatactggc
481 tggctagatc actggggcca acctcactcc acaatcaaga ccgaagcagt ggcttcctcc
541 ctctatgata tacttgcccg tggggcgagt gtgaacttgt acatgtttat aggtgggacc
601 aattttgcct attggaatgg ggccaactca ccctatgcag cacagcccac cagctacgac
661 tatgatgccc cactgagtga ggctggggac ctcactgaga agtattttgc tctgcgaaac
721 atcatccaga agtttgaaaa agtaccagaa ggtcctatcc ctccatctac accaaagttt
781 gcatatggaa aggtcacttt ggaaaagtta aagacagtgg gagcagctct ggacattctg
841 tgtccctctg ggcccatcaa aagcctttat cccttgacat ttatccaggt gaaacagcat
901 tatgggtttg tgctgtaccg gacaacactt cctcaagatt gcagcaaccc agcacctctc
961 tcttcacccc tcaatggagt ccacgatcga gcatatgttg ctgtggatgg gatcccccag
1021 ggagtccttg agcgaaacaa tgtgatcact ctgaacataa cagggaaagc tggagccact
1081 ctggaccttc tggtagagaa catgggacgt gtgaactatg gtgcatatat caacgatttt
1141 aagggtttgg tttctaacct gactctcagt tccaatatcc tcacggactg gacgatcttt
1201 ccactggaca ctgaggatgc agtgcgcagc cacctggggg gctggggaca ccgtgacagt
1261 ggccaccatg atgaagcctg ggcccacaac tcatccaact acacgctccc ggccttttat
1321 atggggaact tctccattcc cagtgggatc ccagacttgc cccaggacac ctttatccag
1381 tttcctggat ggaccaaggg ccaggtctgg attaatggct ttaaccttgg ccgctattgg
1441 ccagcccggg gccctcagtt gaccttgttt gtgccccagc acatcctgat gacctcggcc
1501 ccaaacacca tcaccgtgct ggaactggag tgggcaccct gcagcagtga tgatccagaa
1561 ctatgtgctg tgacgttcgt ggacaggcca gttattggct catctgtgac ctacgatcat
1621 ccctccaaac ctgttgaaaa aagactcatg cccccacccc cgcaaaaaaa caaagattca
1681 tggctggacc atgtatgatg atgagagcct gtgtctttga gggattctac cctgaacata
1741 cctcacagat cctcc
//