LOCUS AK298976 2262 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ59737 complete cds.
ACCESSION AK298976
VERSION AK298976.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2262)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2262
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2018265"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493600"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 182..1336
/codon_start=1
/protein_id="BAG61070.1"
/transl_table=1
/translation="MEQEREGFSEEVTFQIQEMVHSEVAAYDSGRPGPLLGRPAMLAS
HMSALSQSQLISQMGIRSSIAHSSPSPPGSKSATPSPSSSTQEEESEVHFKISGEKRP
SADPGKKAKNPKKKKKKDPNEPQKPVSAYALFFRDTQAAIKGQNPSATFGDVSKIVAS
MWDSLGEEQKQAYKRKTEAAKKEYLKALAAYRASLVSKSSPDQGETKSTQANPPAKML
PPKQPMYAMPGLASFLTPSDLQAFRSGASPASLARTLGSKSLLPGLSASPPPPPSFPL
SPTLHQQLSLPPHAQGALLSPPVSMSPAPQPPVLPTPMALQVQLAMSPSPPGPQDFPH
ISEFPSSSGSCSPGPSNPTSSGDWDSSYPSGECGISTCSLLPRDKSLYLT"
BASE COUNT 495 a 731 c 601 g 435 t
ORIGIN
1 gtttccaaga gggaagcagg aggctggctt cacttggttc ccagacatct catgagtgcc
61 ggctgcctgc ccagccctct gcctggtgct gggaggcagg tgtctctgca cttgagcaga
121 tgccgggagg caacgtgtgg agagctctgc cagaggcttg gaaagagggt gaggccagag
181 gatggagcag gagagggaag gcttctctga ggaggtgaca tttcagatcc aggagatggt
241 ccactcggaa gtggctgcct atgactcggg ccggcccggg cccctgctgg gtcgcccggc
301 aatgctggcc agccacatga gtgccctcag ccagtcccag ctcatctcgc agatgggcat
361 ccggagcagc atcgcccaca gttccccatc accgccgggg agcaagtcag cgaccccctc
421 tccctccagc tccactcagg aagaggagtc ggaagtgcat ttcaagatct cgggagaaaa
481 gagaccttca gccgacccag gaaaaaaggc caagaacccg aagaagaaga aaaagaagga
541 ccccaatgag ccgcagaagc ctgtgtcggc ctacgcactc ttcttcagag acactcaggc
601 cgccatcaag ggtcagaacc ccagtgccac tttcggtgac gtgtccaaaa tcgtggcctc
661 catgtgggac agcctgggag aggaacagaa gcaggcctac aagaggaaga cagaagcagc
721 aaagaaggaa tatctgaagg ccctggcagc ctaccgggct agcctcgtct ccaagagctc
781 cccagatcaa ggtgagacca agagcactca ggcaaaccca ccagccaaaa tgctcccacc
841 caagcagccc atgtatgcca tgccaggcct ggcctccttc ctgacgccgt cggacctgca
901 ggccttccgc agtggggcct cccctgccag cctcgcccgg acgctgggct ccaagtctct
961 gctgccaggc ctcagtgcgt ccccgccgcc gccaccctcc ttcccgctca gccccacact
1021 gcaccagcag ctgtcactgc cccctcacgc ccagggcgcc ctcctcagtc cacctgttag
1081 catgtcccca gccccccagc cccctgtcct gcccaccccc atggcactcc aggtgcagct
1141 ggcgatgagc ccctcacctc cagggccaca ggacttcccg cacatctctg agttccccag
1201 cagctcggga tcctgctcac ctggcccatc caaccccacc agcagcgggg actgggacag
1261 cagctacccc agtggggagt gtggcatcag cacctgcagc ctgctcccca gggacaaatc
1321 gctctacctc acctaatccc gcctccctac catccctgag gctcgctgga aggcactgct
1381 cagagcctga agggctgaca gcagaaaaga ggccctggcc agaggcaggg tggcccatcg
1441 gagagagcag tgacacaccc attgcccggg ggctgagtct cttcctcaac ctcccaccag
1501 actctgcaga ggcagcccac tgcccaccac cagcccaaag aacctgcagg aaccttccgc
1561 ccgctgacct gcttgctcca gggtaactgt ggaccctgtc ctcgccctgc gcacggtacc
1621 ctatgtctgg acacccggcc ccagctccag ccccagccca ggtgggccgc ccctggcggg
1681 gtcgcttacc aacggacacc caccccagat gcatgggcca gagggccggc ccccggcata
1741 gatgtgcaca tcggttttcc agtgtgaaca aaagattacg aaacctagaa actgttggtt
1801 ccgtgtaagt agttgactac gtgttttaga actgtgctga agacatctgt aagactattt
1861 tgtgggggaa aaaagtagtt tcctttaagg taaaaagcat tttatatgat ccttagcaca
1921 tttttaagtt ttatcttaag ggagacgcgc acaaaagcgg ctgccaaacc gtttcgtcat
1981 cctcacagca aggaccggac gcttgctagc caccccggag cactgctctc cttttaatca
2041 tgtattcatc tattttaaat tgccggcgac gacttttgtc tatttatgaa gaaaccttga
2101 gaacgaagtt acagcttatc ctaccgtgtg tgtggttttg gggtttcgtt tgggtttggg
2161 ttcttgacgt cgtttgcagc tgtttcctgg ccctggcgag tgtctgtctt ggtgcccagt
2221 gcttctctca aatctcttta taataaaact tctgaaaagc tg
//