LOCUS AK298971 1470 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ55793 complete cds, highly similar to Homo
sapiens zinc finger protein 532 (ZNF532), mRNA.
ACCESSION AK298971
VERSION AK298971.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1470)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1470
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2017559"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493595"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 23..1066
/codon_start=1
/note="highly similar to Homo sapiens zinc finger protein
532 (ZNF532), mRNA"
/protein_id="BAG61065.1"
/transl_table=1
/translation="MMDHIKSMHGTLKSIEGPPNLGINLPLSIKPATQNSANQNKEDT
KSMNGKEKLEKKSPSPVKKSMETKKVASPGWTCWECDCLFMQRDVYISHVRKEHGKQM
KKHPCRQCDKSFSSSHSLCRHNRIKHKGIRKVHCPDSRRTFTKRLMLEKHVQLMHGIK
DPDLKEMTDATNEEETEIKEDTKVPSPKRKLEEPVLEFRPPRGAITQPLKKLKINVFK
VHKCAVCGFTTENLLQFHEHIPQHKSDGSSYQCRECGLCYTSHVSLSRHLFIVHKLKE
PQPVSKQNGAGEDNQQENKPSHEDESPDGAVSDRKCKVCAKTFETEAALNTHMRTHGM
AFIKSKRMSSAEK"
BASE COUNT 466 a 321 c 321 g 362 t
ORIGIN
1 tttatatgca cagaagcaac ttatgatgga ccatatcaag tctatgcatg gaacattgaa
61 aagtattgaa gggcctccaa acttgggtat aaacttgcct ttgagcatta agcctgcaac
121 tcaaaattca gcaaatcaga acaaagagga caccaaatcc atgaatggga aagagaaatt
181 ggaaaagaaa tctccgtctc ctgtgaaaaa atcaatggaa accaagaaag tggccagtcc
241 tgggtggacg tgttgggagt gtgactgcct gttcatgcag agagatgtgt acatatccca
301 cgtgaggaag gagcacggga agcaaatgaa gaaacacccc tgccgccagt gtgacaagtc
361 tttcagctcg tcccacagcc tgtgccggca caaccggatc aagcacaaag gcatcaggaa
421 agtgcactgc ccagactcca gacgtacctt taccaaacgt ttgatgctgg agaagcacgt
481 ccagctgatg catggcatca aggaccctga cctgaaagaa atgacagatg ccaccaatga
541 ggaggaaaca gaaataaaag aagacactaa ggtccccagt cccaagcgga agttggaaga
601 accagttctg gagttcaggc ctccccgagg agcaatcact caaccactga aaaagctgaa
661 aatcaatgtt tttaaggttc acaagtgtgc cgtgtgtggc ttcaccaccg aaaacctgct
721 gcaattccac gaacacatcc ctcagcacaa atcggatggt tcttcctacc agtgccggga
781 gtgtggcctc tgctacacgt ctcacgtctc tctgtccagg cacctcttca tcgtacacaa
841 gttaaaggaa cctcagccag tgtccaagca aaatggggct ggggaagata accaacagga
901 gaacaaaccc agccacgagg atgaatcccc tgatggcgcc gtgtcagaca gaaagtgcaa
961 agtgtgcgca aaaacttttg aaactgaagc tgccttaaat actcacatgc ggacacacgg
1021 catggccttc atcaaatcca aaaggatgag ctcagccgag aaatagccac agatgctcca
1081 tgaggaaaat ccctgtccac attggaataa aaaagacatt tttgttacaa agtttgcagt
1141 ataatagagt taacagtact gtctaggctg ttgcaatata ttctctttca atgtaccttc
1201 cttcacctcg tcgtatatat cctcgataag tattaaaaca gtatttgagt ttaaaagagt
1261 ttgtatatat ttaaatgaat aactttttat actctttgtt acatgtttgt atcagtattt
1321 agtggaaaac catttgagtt gttttgggtt agaatttttc tttttgtact gtttctttaa
1381 aacagagttc ttagtaacag gggcagttcc tgaattcaaa taaaccattt tgtatgtttg
1441 gattttgaat gggttaacta attacaggct
//