LOCUS AK298970 2180 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ53162 complete cds, moderately similar to Mus
musculus SH3-domain GRB2-like (endophilin) interacting protein 1
(Sgip1), mRNA.
ACCESSION AK298970
VERSION AK298970.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2180)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2180
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2017487"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493594"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 220..2076
/codon_start=1
/note="moderately similar to Mus musculus SH3-domain
GRB2-like (endophilin) interacting protein 1 (Sgip1),
mRNA"
/protein_id="BAH12914.1"
/transl_table=1
/translation="MMEGLKKRTRKAFGIRKKEKDTDSTGSPDRDGINSPELDEEGYS
IRPEEPGSTKGKHFYSSSESEEEEQSHKKFNIKIKPLQSKDILKNAATVDELKASIGN
IALSPSPVRKSPRRSPGAIKRNLSSEEVARPRRSTPTPELISKKPPDDTTALAPLFGP
PLESAFDEQKTEVLLDQPEIWGSGQPINPSMESPKLTRPFPTGTPPPLPPKNVPATPP
RTGSPLTIGPGASSPARPATPLVPCRSTTPPPPPPRPPSRPKLPPGKPGVGDVSRPFS
PPIHSSSPPPIAPLARAESTSSISSTNSLSAATTPTVENEQPSLVWFDRGKFYLTFEG
SSRGPSPLTMGAQDTLPVAAAFTETVNAYFKGADPSKCIVKITGEMVLSFPAGITRHF
ANNPSPAALTFRVINFSRLEHVLPNPQLLCCDNTQNDANTKEFWVNMPNLMTHLKKVS
EQKPQATYYNVDMLKYQVSAQGIQSTPLNLAVNWRCEPSSTDLRIDYKYNTDAMTTAV
ALNNVQFLVPIDGGVTKLQAVLPPAVWNAEQQRILWKIPDISQKSENGGVGSLLARFQ
LSEGPSKPSPLVVQFTSEGSTLSGCDIELVGAGYRFSLIKKRFAAGKYLADN"
BASE COUNT 639 a 555 c 462 g 524 t
ORIGIN
1 cttttctctc agcatcttct tggtagcctg cctgtaggtg aagaagcacc agcagcatcc
61 atggcctgtc ttttggctta acacttatct cctttggctt tgacagcgga cggaatagac
121 ctcagcagcg gcgtggtgag gacttagctg ggacctggaa tcgtatcctc ctgtgttttt
181 tcagactcct tggaaattaa ggaatgcaat tctgccacca tgatggaagg attgaaaaaa
241 cgtacaagga aggcctttgg aatacggaag aaagaaaagg acactgattc tacaggttca
301 ccagatagag atggaattaa ctcacctgag ctggatgaag aaggctacag catcagaccc
361 gaggaacccg gctctaccaa aggaaagcac ttttattctt caagtgaatc ggaagaagaa
421 gaacaatcac ataagaaatt taatatcaag attaaaccat tgcaatctaa agacattctt
481 aagaatgctg caactgtaga tgaattgaag gcatcaatag gcaacatcgc actttcccca
541 tcaccagtga ggaaaagtcc gaggcgcagc ccgggtgcaa ttaaaaggaa cttatccagt
601 gaagaagtgg caagacccag gcgttccaca ccaactccag aacttataag caaaaagcct
661 ccagatgaca ctacggccct tgctcctctc tttggcccac cactagaatc agcttttgat
721 gaacagaaga cagaagttct tttagatcag cctgagatat ggggttcagg ccaaccaatt
781 aatccaagca tggagtcgcc aaagttaaca aggccttttc ccactggaac acctccacca
841 ctgcctccaa aaaatgtacc agctacccca ccccgaacag gatccccctt aacaattgga
901 ccaggtgcat catcccctgc tcgaccagcc actcctttgg ttccttgcag aagtaccact
961 ccacctccac ctcctccccg gcctccatcc cggccaaagc tacctccagg aaaacctgga
1021 gttggagatg tgtccagacc ttttagccct cccattcatt cttccagccc tcctccaata
1081 gcacccttag cgcgggctga aagcacttct tcaatatcgt caaccaattc cttgagcgca
1141 gccaccactc ccacagttga gaatgaacag ccttccctcg tttggtttga cagaggaaag
1201 ttttatttga cttttgaagg ttcttccagg ggacccagcc ccctaaccat gggagctcag
1261 gacactctcc ctgttgcagc agcatttaca gaaacagtca atgcctattt caaaggagca
1321 gacccaagca aatgtatcgt taagattacc ggagaaatgg tgttgtcatt tcctgctggc
1381 atcaccagac actttgccaa caacccgtcc ccagctgctc tgacttttcg ggtgataaat
1441 ttcagcaggt tagaacacgt cctgccaaac ccccaacttc tctgctgtga taatacacaa
1501 aatgatgcca ataccaagga attctgggta aacatgccaa atttgatgac tcacctaaag
1561 aaagtgtctg aacaaaaacc ccaggctaca tattataacg ttgacatgct caaatatcag
1621 gtgtctgccc agggcattca gtccacacct ctgaacctgg cagtgaattg gcgatgtgag
1681 ccttcaagca ctgacctgcg catagattac aaatataata cagatgcaat gacgactgct
1741 gtggccctca acaatgtgca gttcctggtc cccatcgacg gaggagtcac caagctccag
1801 gcagtgctcc caccagcagt ctggaatgct gaacaacaga gaatattgtg gaagattcct
1861 gatatctctc agaagtcaga aaatggaggg gtgggttctt tgttggcaag atttcagtta
1921 tctgaaggcc caagcaaacc ttctccattg gttgtgcagt tcacaagtga aggaagcacc
1981 ctttctggct gtgacattga acttgttgga gcagggtatc gattttcact catcaagaaa
2041 aggtttgctg caggaaaata cttggcagat aactaatgaa atcttatgca aggatttgga
2101 ggattcatat aatggagaac tgatgtatga gaaacagatt ttaattttgg tttgatgaaa
2161 acaaaccaat atctgcactt
//