LOCUS AK298969 1179 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ54853 complete cds, highly similar to
Biogenesis of lysosome-related organellescomplex-1 subunit 2.
ACCESSION AK298969
VERSION AK298969.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1179)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1179
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2017411"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493593"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 22..450
/codon_start=1
/note="highly similar to Biogenesis of lysosome-related
organellescomplex-1 subunit 2"
/protein_id="BAG61064.1"
/transl_table=1
/translation="MAAAAEGVLATRSDEPARDDAAVETAEEAKEPAEADITELCRDM
FSKMATYLTGELTATSEDYKLLENMNKLTSLKYLEMKDIAINISRNLKDLNQKYAGLQ
PYLDQINVIEEQVAALEQAAYKLDAYSKKLEAKYKKLEKR"
BASE COUNT 365 a 213 c 272 g 329 t
ORIGIN
1 aaaaacagcg cggggtccgc tatggcggcg gcagccgagg gcgtactggc gacccggagt
61 gatgagcccg cccgagacga tgccgccgtg gagacagctg aggaagcaaa ggagcctgct
121 gaagctgaca tcactgagct ctgccgggac atgttctcca aaatggccac ttacctgact
181 ggggaactga cggccaccag tgaagactat aagctcctgg aaaatatgaa taaactcacc
241 agcttgaagt atcttgaaat gaaagatatt gctataaaca ttagtaggaa cttaaaggac
301 ttaaaccaga aatatgctgg actgcagcct tatctggatc agatcaatgt cattgaagag
361 caggtagcag ctcttgagca ggcagcttac aagttggatg catattcaaa aaaactggaa
421 gccaagtaca agaagctgga gaagcgatga gaaacttatt tctatgggac agagtctttt
481 ttttttaatg tggaagaatg tcttataaaa cctgaatcct gaggctgatg aattgtgaaa
541 attcctcaaa aggaaattat gctggtcatc acaggaacat ctcaacgttc gagtaaactg
601 gaggactgtg gctattcctg aaccttcttt gagacagaat ccctcagaat ctcacactta
661 taacttccta ccttttactt gaatgctttg ccatattcag gacagagact ctcacaaagt
721 tcagaaaaca gctggactta ccagtaaaat caaatgagag gacctatttt ctctggtagt
781 ggttgattac tacattattt tcttaagtgg ctggtttttt agttactatg taaatggtcg
841 tttttctgtt aatgatgcta atgtgttgta aacaagattc taaatttaaa aaggaaaaca
901 aaacaaactt gttctttgca gcttatcacc ttgtgaatgt cggtaactta cttttccata
961 atattgcaaa taacataaaa tcttaaaata attccaagct gagtcttcta gattgagcag
1021 aaatggtgaa aggagtattg ataacttggc gtatgtgatg ggcccctctt gtttattttc
1081 tatgtgagtc acattgacat gcgatcagtt tgggaaatgt gatgaaaaca aagactagat
1141 gggtatgtgt gtttatgtgt tgggtaggga ggtgacgat
//