LOCUS AK298966 3468 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ52095 complete cds.
ACCESSION AK298966
VERSION AK298966.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3468)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3468
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2017260"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493590"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 186..3329
/codon_start=1
/protein_id="BAG61061.1"
/transl_table=1
/translation="MEEKTQIKTFLGSKLPKYGTKSVRSTLQPMPNGTPVNLLGTSKN
SNVKSYIKNNGSDCPSSHSFNWRKANKYQLCAQGVEEPNNTQNSHDKIIDPEKRVPTQ
GMFDKNGIKGGLKSVSLFTSKLAKPSTMFVSSTEELNQKSFSGPSNLGKFTKGTLLGR
TSYSSINTPKSQLNGFYGNRSAGSMQRPRANSCATRSSSGESLAQSPDSSKSINCEKM
VRSQSFSHSIQNSFLPPSSITRSHSFNRAVDLTKPYQNQQLSIRVPLRSSMLTRNSRQ
PEVLNGNEHLGYGFNRPYAAGGKKLALPNGPGVTSTLGYRMVHPSLLKSSRSPFSGTM
TVDGNKNSPADTCVEEDATVLAKDRAANKDQELIENESYRTKNNQTMKHDAKMRYLSD
DVDDISLSSLSSSDKNDLSEDFSDDFIDIEDSNRTRITPEEMSLKEEKHENGPPQDMF
DSPKENEKAFSKTDEWIDISVSDRSECTKHTSGNNLVSPDTDYRAGSSFELSPSDSSD
GTYMWDEEGLEPIGNAHPVGSYESSEMNSIDVLNNLESCDLEDDDLMLDVDLPEDAPL
ENVECDNMNRFDRPDRNVRQPQEGFWKRPPQRWSGQEHYHLSHPDHYHHHGKSDLSRG
SPYRESPLGHFESYGGMPFFQAQKMFVDVPENTVILDEMTLRRMVQDCTAVKTQLLKL
KRLLHQHDGSGSLHDIQLSLPSSPEPEDGDKVYKNEDLLNEIKQLKDEIKKKDEKIQL
LELQLATQHICHQKCKEEKCTYADKYTQTPWRRIPPQVLQPFSSLPRPTDHTQGKLIK
PQRIEARSECSIQDMHQGGAHPEESFTHVLHQESNYGLEEQPFSSGPQLTMDVAKSTP
SEANLNITVNAQEPYHLANNQISDIQFIPTSLQTPPESSTVDQAKRVGRNQSPPVGYM
SQPKSLQLLKPSILSSLVPPPVSESSPSRTPTCKKSPIITTCNSAKLQPTSSQTNLAN
NQNLKASKLRPPSGSFKQKQTNSPQLEPQSFQAKTSIPRPLTQRKEIMQNPNGNLHSG
DCLASNRYSRLPKPKIH"
BASE COUNT 1130 a 745 c 708 g 885 t
ORIGIN
1 gcgatgctgg ttgctgcggc tcgggcggcg gggctggcgg ggatgtgacg ggcggccctt
61 cgtctcacct tccgtcctcg ggcccgcagg tcgcagggcg gcctgcagct gggccgcggc
121 cgaggaggca gcgcgacctc cgcacgattc tttcaacttt taagaacaaa tgcaccttat
181 agctcatgga agaaaaaaca caaatcaaga catttttggg ttccaagttg ccaaagtatg
241 gaacaaaatc tgtaagaagt acattgcagc caatgccaaa tgggacacct gttaatttat
301 taggaacttc caagaatagt aatgtcaaaa gttacatcaa aaataatggc tctgattgtc
361 catcatctca ttcatttaat tggaggaaag caaataaata tcagctttgt gcacaaggtg
421 tcgaagagcc taacaatact caaaattcac atgataaaat aattgatcct gaaaaacgtg
481 ttcctactca aggaatgttt gataaaaatg ggataaaggg aggtttgaaa agtgtttctt
541 tattcacatc aaagttagca aagccatcca ctatgtttgt gtcatctaca gaggagttaa
601 accaaaagtc tttttctgga ccatctaatt tgggtaaatt caccaaaggc acattattag
661 gaaggacttc atattcttcg atcaatactc caaaatcaca gttgaatgga ttttatggaa
721 accgatcagc tggtagcatg caaaggccta gagcgaactc ctgtgccacc agaagcagtt
781 ctggagaaag cttagctcaa tccccagaca gtagtaaatc tattaattgt gaaaaaatgg
841 taaggtcaca aagtttttca cattccattc agaattcatt ccttccacct tcatctataa
901 ccagatcaca ttcctttaat agagctgtgg atcttacaaa gccttatcag aaccaacagc
961 tatccattag agtgcctcta cggtcaagta tgctaacaag aaattcccgg cagccagaag
1021 tactcaatgg gaatgaacat ttggggtatg gatttaatag gccttatgct gctggtggaa
1081 agaagttggc tttaccaaat ggcccaggtg taacttctac tttaggttat agaatggttc
1141 atccctctct actgaaatct agccgatctc cattttctgg gactatgaca gttgatggaa
1201 ataaaaattc acctgctgac acatgtgtag aggaagatgc tacagttttg gctaaggaca
1261 gagctgctaa taaggaccaa gaactgattg aaaatgaaag ttatagaaca aaaaacaacc
1321 agaccatgaa acatgatgct aaaatgagat acctgagtga tgatgtggat gacatttcct
1381 tgtcgtcttt gtcatcttct gataagaatg atttaagtga agactttagt gatgatttta
1441 tagatataga agactccaac agaactagaa taactccaga ggaaatgtct ctcaaagaag
1501 agaaacatga aaatgggcca ccacaggata tgtttgattc ccccaaggaa aatgaaaaag
1561 ccttcagtaa aactgatgaa tggatagata taagtgtctc tgacaggagt gaatgtacaa
1621 aacatacttc tgggaataat ttggtttcac cagatacaga ctacagagct ggttcttcgt
1681 ttgaactctc tccatctgat agctctgatg gaacatacat gtgggatgaa gaaggcttgg
1741 aacccattgg aaatgcccat ccagttggga gctatgagtc ctctgaaatg aacagcatag
1801 atgttttgaa taatcttgaa tcatgtgacc ttgaggatga tgatcttatg cttgatgtgg
1861 atctgcctga ggatgcacct cttgaaaatg tggagtgtga caatatgaac cgctttgacc
1921 gaccagacag aaatgttcgg cagcctcagg aaggtttttg gaaaaggcca ccccagaggt
1981 ggagtggaca ggagcattac cacctcagcc accctgacca ctatcatcac catggaaaaa
2041 gtgacttgag cagaggctct ccctatagag aatctccttt gggtcatttt gaaagctatg
2101 gagggatgcc ctttttccag gctcagaaga tgtttgttga tgtaccagaa aatacagtga
2161 tactggatga gatgaccctt cggcgcatgg ttcaggattg cactgctgta aaaactcagt
2221 tactcaaact gaaacgtctc ctgcatcagc atgatggaag tggttcattg catgatattc
2281 aactgtcatt gccatccagt ccagaaccag aagatggtga taaagtatat aagaatgaag
2341 atttattaaa tgaaataaaa caacttaaag acgaaataaa gaaaaaagat gaaaagatcc
2401 aactattaga acttcagctt gcaactcagc atatctgcca ccaaaaatgt aaagaggaaa
2461 aatgcactta tgctgataaa tatacccaaa caccctggag acgaattcct cctcaagtac
2521 tacagccttt cagcagcctt cccagaccca cagatcacac ccagggaaaa ctaataaagc
2581 cacaacgtat cgaggcccgc agtgaatgct caatccaaga catgcatcag ggcggtgcac
2641 atccggaaga aagctttaca cacgtcttgc accaagaaag caactatggt ttggaagagc
2701 agcctttttc atcaggccca caattaacaa tggatgtggc taagagtaca ccttctgaag
2761 caaacttaaa cattactgta aatgctcaag agccttatca tttggcaaac aatcaaatta
2821 gtgacataca gtttataccc acttctcttc agacacctcc cgagtcaagt acagtagacc
2881 aggctaagag agttggaaga aatcagtctc cgccagtggg ttatatgtct cagcccaagt
2941 ccttgcagct tttaaagcca tccatattga gttctttggt accgcctcca gtttctgaat
3001 catctccaag taggactccc acttgtaaaa agtcaccaat aatcacaaca tgtaattcag
3061 caaaacttca gccaacatct agtcaaacaa atcttgcaaa taatcagaat ctgaaagcat
3121 ctaagctccg ccccccctca ggctctttca aacaaaaaca aacaaacagc ccccaactag
3181 agcctcaaag cttccaggcc aagacaagca tcccaaggcc actaacacaa cgaaaagaaa
3241 tcatgcagaa tccaaatggc aatttgcatt ctggggattg tttggcctct aatcgatatt
3301 ctcgtcttcc taaaccaaag atacattaag tacatagcca tcacctgcca atttgtttct
3361 taaaaacaat ctcttctgta atagctttat gtgcagcttg cagcttgcta ctgtggtgga
3421 ggttccattg aaagcctgca aatcttaaat taaaatgtgg aagcttct
//