LOCUS AK298940 1171 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ50620 complete cds, weakly similar to Protein
FAM86A.
ACCESSION AK298940
VERSION AK298940.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1171)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1171
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2013923"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493564_01"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 236..625
/codon_start=1
/note="weakly similar to Protein FAM86A"
/protein_id="BAG61040.1"
/transl_table=1
/translation="MPPSTLQNGPSRTRQPSLTDVLYCPEAIVSLVGVLQRLAACREH
KRAPEVYVAFTVRNPETCQLFTTELGEPPRPPGPTWCPSCPCRTPVEVKELGAGEKLG
CSTLPHYAGNSGRGRPGWDQMGSRSSS"
BASE COUNT 278 a 359 c 297 g 237 t
ORIGIN
1 ctcttcagtc caggcccggc ttcctccagg tctcccggca acggctgcgg cctcgcccac
61 gttatggcgc ccgaggagaa cgcggggacc gaactcttgc tgcagggttt tgagcgccgc
121 ttcctggcgg tgcgcacact gcgctccttc ccctggcagt cctcaggagg ctcagtcaca
181 ctctccaaga gcacagccat catctcccac ggtaccacag gcctggtcac atgggatgcc
241 gccctctacc ttgcagaatg ggccatcgag aacccggcag ccttcattaa cagacgtgct
301 gtattgccca gaagccatcg tgtcgctggt cggggtcctg cagaggctgg ctgcctgccg
361 ggagcacaag cgggctcctg aggtctacgt ggcctttacc gtccgcaacc cagagacatg
421 ccagctgttc accaccgagc taggtgaacc cccacgccca cccgggccta catggtgccc
481 gagctgtccc tgcaggactc cagtggaagt gaaagaactg ggcgctgggg aaaagctagg
541 atgctccaca ctcccacact atgcggggaa ctcgggcaga ggccggccgg gatgggatca
601 gatgggaagc agaagctcat catgaccaga aactgtttcc ctatggagag cacttggaga
661 tggcaatgct gaacctcaca ctgtaggact cacacatgac tccaaaggga ttgtgagaat
721 caagtcactc tcgtgggaag aatttttata tgggaaagtg gataaaactt tcattggact
781 ggaatgtttg gagattatta aactccaaat caggaatcaa aactgccctc taataagaca
841 ttagctgtct aggcgtgtgg gtgccccctt tctgccagca gttctggttc tcaagaaaat
901 catcataaat cagacatgaa aattctagct ccaaaaatag cattttcttt ctgcaaataa
961 aaacgtgtgt atcaaggatg acgttccccc aacgtggaca cactcggttc ctcagaaagc
1021 caagcccgct gcagctgcca catccctgga cacactcggt tcctcacaaa gccaagcccg
1081 ctgcagctgc cacatccctg gacacactcg gttcctcaca aagccaagcc cgctgcagct
1141 gccacatccc tggacacact cggttcctca c
//