LOCUS AK298931 1130 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ57077 complete cds.
ACCESSION AK298931
VERSION AK298931.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1130)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1130
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2012231"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493555"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 100..546
/codon_start=1
/protein_id="BAG61034.1"
/transl_table=1
/translation="MKSRKEQRLLTGPSPLMTAALGTTSLLSIATTLACGLLLVEEAI
NENEYHSGNLGWSDLIFSHSGHRYFRPPRGSLTLSILLAPLFEELGTISPGTGLERQN
LTMLPRLVSNSWTQVILLPWPPKVLELQELASLLSDQPQNFLPLIP"
BASE COUNT 316 a 273 c 226 g 315 t
ORIGIN
1 aaccaacgtg ccgcccctct gtgggcgggg attcacacta gctgtctgtg tcaaggccac
61 gcagaaatct attggaaatc gctgtggaaa caaacgcaaa tgaaaagccg caaagagcaa
121 aggctgctca ctggcccgtc tcctctcatg actgctgctc tgggaaccac ctctctgctc
181 tccatcgcta ccactctagc ctgtggcctg ctcctggttg aagaagccat caatgaaaat
241 gaataccact ctggaaatct tgggtggtct gacctgatat tttcgcatag tggccacaga
301 tattttaggc ccccaagagg tagtcttact ctttcaattt tactggctcc actgtttgag
361 gaacttggaa caatttcccc agggactggt ctggagagac agaatctcac tatgttgccc
421 aggctggtct cgaactcttg gactcaagtg atcctcctgc cttggcctcc caaagtgctg
481 gaattacagg aattagcctc cttactgtca gaccagccac agaacttcct cccacttatc
541 ccttagatgt caactaccca cacttcaaat caccctggag actgatgtga ctgggatgat
601 ttgactgcat gtaggtcaga gctgcctcca ggcaacttgt ggggccaagg ctctgtggct
661 agaaggaagt gagcattaat acatcactta gaacagcaaa aatcatcatc tgaagatcat
721 catctgaaaa gatcagcatc agaagagctg tgaatatcac cgatctgata attgggtatt
781 tttggtaaaa cacacacaca cacacacaca cacacacaca cacacgcaca gatgcacaca
841 gccctgcttt tatgagaatt gagcttaaat agatgtgggt ggaggtttta ctgaactgga
901 tatttcttaa cttttggagt acaacaccta gtctcctaat tcctgtcctg catagcgtgc
961 ttacacagta ttttaaaatt agagataatt aaatttgatt ttgtttcaat aattattttg
1021 acagttttaa aatatctttt ggaattgttt gtaccattcc agtccttttc aaatccaaga
1081 ctgaaatgaa tttttctagc tatttatccc atctttgagt taagaacata
//