LOCUS AK298930 1332 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ51529 complete cds.
ACCESSION AK298930
VERSION AK298930.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1332)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1332
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2011084"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493554"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 63..491
/codon_start=1
/protein_id="BAH12905.1"
/transl_table=1
/translation="MFFSEARARSRTWEASPSEHRKWVEVFKACDEDHKGYLSREDFK
TAVVMLFGYKPSKIEVDSVMSSINPNTSGILLEGFLNIVRKKKEAQRYRNEVRHIFTA
FDTYYRGFLTLEDFKKAFRQVAPKLPERTVLEVFRGIFSA"
BASE COUNT 367 a 252 c 314 g 399 t
ORIGIN
1 agccaggctg ggaaagcggt ggtagctggt tgggttgctg gggctcggtt gttgtagtcg
61 cgatgttctt ctccgaggcc agagccaggt cgcggacgtg ggaagccagt ccctcggaac
121 acaggaagtg ggtggaagta tttaaagcat gtgatgaaga tcacaaagga tatctcagca
181 gagaggactt taaaactgct gttgtaatgc tgtttgggta caagccctcc aagatagaag
241 tggattctgt gatgtcttca ataaatccaa atacttctgg tatattactc gaggggtttt
301 taaatattgt caggaaaaag aaggaagctc aacgatatcg gaacgaagta agacacatct
361 tcacagcctt tgacacctac tatcgtggat ttttaacttt ggaagatttc aaaaaagcat
421 ttaggcaggt ggctcccaaa ttaccggaaa ggactgttct tgaggtattc agaggcattt
481 tcagtgcatg atttaacttc ggctgtcatc aggaagctga cttaaagttc tttcttgact
541 ttgcctgcca tcaacaccct gtggtatttc tggctggcat ttttctctgc atggatatgt
601 ttacttctcc atgcctagga accttctcca tctcattgta agcactgcta tttgtacatt
661 cccaccacct gtcagttggc aaaaagctct gggaagctgc ttttgcttcc cagtcatgag
721 atgaaatgtg aatgtgcata aggtccatgc cctgacccag cgtccaagat cctgagtgca
781 caggcagatt gccacatgat gatgtaaagt ggcaatatcg aggcaccaaa tgctcccatc
841 atgactggag acgtttaaag ttatatcctg atatttttgg tgtaagaatc cctttcttca
901 tattacacaa gggaaggtca aaggacatag gacatctaac tctccttttt tgaatattag
961 aaattgggtg aaaatgagaa tgtcattgct tgttgaataa gatattttca aaatcagagt
1021 attagtgggg gctgggtctt aggaatgcaa ttgtaaaagg gagtgatgga tattcccttg
1081 gcagaaaaat tatatagatg ttatatcaca gccattatag gggtaaggac tgtcaccagc
1141 ttgggtaaaa caaataggct cttctgttat gctgattgga agagtcttac agagctgagg
1201 gcctaaagga aagtgatttc agagaacatt tactttttca agtcatttgc gtctcttttg
1261 aaatgttatt tctcactgta atttttagct tcccataatt cctttctgcc agcagccaac
1321 ttgtggagta tc
//