LOCUS AK298918 1967 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ57562 complete cds, highly similar to
Cleavage and polyadenylation specificityfactor 73 kDa subunit.
ACCESSION AK298918
VERSION AK298918.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1967)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1967
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2008103"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493542"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 665..1885
/codon_start=1
/note="highly similar to Cleavage and polyadenylation
specificityfactor 73 kDa subunit"
/protein_id="BAG61024.1"
/transl_table=1
/translation="MAVYQTYVNAMNDKIRKQININNPFVFKHISNLKSMDHFDDIGP
SVVMASPGMMQSGLSRELFESWCTDKRNGVIIAGYCVEGTFAKHIMSEPEEITTMSGQ
KLPLKMSVDYISFSAHTDYQQTSEFIRALKPPHVILVHGEQNEMARLKAALIREYEDN
DEVHIEVHNPRNTEAVTLNFRGEKLAKVMGFLADKKPEQGQRVSGILVKRNFNYHILS
PCDLSNYTDLAMSTVKQTQAIPYTGPFNLLCYQLQKLTGDVEELEIQEKPALKVFKNI
TVIQEPGMVVLEWLANPSNDMYADTVTTVILEVQSNPKIRKGAVQKVSKKLEMHVYSK
RLEIMLQDIFGEDCVSVKDDSILSVTVDGKTANLNLETRTVECEEGSEDDESLREMVE
LAAQRLYEALTPVH"
BASE COUNT 606 a 393 c 439 g 529 t
ORIGIN
1 gtgctcttgg tgaatggggt tcttcctttt ttatttaccg gtggctgtgc ttccaattta
61 ggaagacccc ggcgacctgt tcctcacccc cgcttcgccc tcacactttc gggatgtctg
121 cgattcctgc tgaggagagc gaccagctgc tgatccgacc ccttggagct gggcaagaag
181 taggaagatc atgtattatt ctcgagttca aaggaagaaa aataatgctc gactgtggga
241 tccaccctgg cctagaagga atggatgctc ttccttatat tgatttaatt gacccagctg
301 agattgatct cctattaatt agtcatttcc atttggatca ctgtggagct ctgccctggt
361 ttctacagaa gacaagtttc aaaggaagaa catttatgac tcatgccaca aaagctattt
421 atagatggct tctttctgat tatgtcaaag ttagtaacat atccatgaga aacgtgaaga
481 gcgagaagca agattctgta acactgtcca cgatattgta aacagaggag gcaggggtct
541 cattcctgtc tttgctcttg gaagggctca ggagctgctc ttgattctag atgagtactg
601 gcagaatcac ccagaactac atgacattcc aatatactat gcatcatctt tggccaagaa
661 gtgtatggca gtgtaccaga catatgtaaa tgccatgaat gacaaaatcc gcaaacagat
721 caacatcaat aatccctttg ttttcaaaca cattagtaac ctcaagagca tggatcattt
781 tgatgacatt ggtcccagtg ttgtaatggc ctccccaggc atgatgcaaa gtggcttatc
841 cagagaatta tttgaaagct ggtgtactga taagaggaat ggtgtcatta tagcgggata
901 ctgtgtagaa gggacatttg ccaagcacat catgtctgaa cctgaagaaa tcactactat
961 gtctggacag aagttaccac tgaaaatgtc tgttgattac atttctttct cagctcacac
1021 ggattaccag caaaccagtg aatttattcg tgctttgaaa ccgcctcatg tgattttagt
1081 ccatggagaa cagaatgaaa tggccagatt gaaagcagca ctgattcgag aatatgaaga
1141 taacgatgaa gttcacatag aggttcataa tcctcggaat acagaagcag tgaccttaaa
1201 cttcagagga gaaaaactag ccaaggttat gggattttta gcagacaaaa aaccagaaca
1261 aggccagcgg gtctcaggaa tacttgttaa aagaaacttt aattatcaca tactttctcc
1321 ttgcgacctg tccaattata ctgacctggc catgagcacg gtgaagcaga cccaagccat
1381 tccatatact ggtcccttta atttgctctg ttaccagctg cagaaattga caggtgatgt
1441 ggaagaatta gaaattcaag aaaaacctgc tctgaaagtg ttcaaaaata ttactgtaat
1501 acaagaacca ggcatggtgg tattagaatg gctggcaaac ccttctaatg atatgtatgc
1561 agatacagta acaactgtga tattggaagt tcagtcaaat cccaaaataa gaaaaggtgc
1621 agtacagaag gtttctaaaa aattagaaat gcacgtttac agcaagaggt tggagatcat
1681 gctccaggac atatttggag aagactgtgt aagtgtaaag gatgactcta ttcttagcgt
1741 cacagtggac gggaaaactg ccaaccttaa cttggagaca cggactgtag aatgtgaaga
1801 gggaagtgaa gacgatgaat ccctccgaga aatggtggag ctggctgcac agagactgta
1861 cgaggccctg acgccagttc actgagactg tgcctgtata tgaactttga aaaaatactt
1921 gactctactt ttgttaccta aaataaaatg cattcgtttc tctggat
//