LOCUS AK298910 1980 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ56227 complete cds, highly similar to Homo
sapiens diacylglycerol lipase beta.
ACCESSION AK298910
VERSION AK298910.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1980)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1980
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2004987"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493534"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 55..1515
/codon_start=1
/note="highly similar to Homo sapiens diacylglycerol
lipase beta"
/protein_id="BAG61018.1"
/transl_table=1
/translation="MIQASYLMASRQQLQACGKPESSSCAVALGKTTILGLLFRDTDL
VPSDIAAGLALLHQQQDNIRNNQEPAQVVCHAPGSSQEADLDAELENCHHYMQFAAAA
YGWPLYIYRNPLTGLCRIGGDCCRSRTTDYDLVGGDQLNCHFGSILHTTGLQYRDFIH
VSFHDKVYELPFLVALDHRKESVVVAVRGTMSLQDVLTDLSAESEVLDVECEVQDRLA
HKGISQAARYVYQRLINDGILSQAFSIAPEYRLVIVGHSLGGGAAALLATMLRAAYPQ
VRCYAFSPPRGLWSKALQEYSQSFIVSLVLGKDVIPRLSVTNLEDLKRRILRVVAHCN
KPKYKILLHGLWYELFGGNPNNLPTELDGGDQEVLTQPLLGEQSLLTRWSPAYSFSSD
SPLDSSPKYPPLYPPGRIIHLQEEGASGRFGCCSAAHYSAKWSHEAEFSKILIGPKML
TDHMPDILMRALDSVVSDRAACVSCPAQGVSSVDVA"
BASE COUNT 445 a 560 c 560 g 415 t
ORIGIN
1 ttggggggaa aatggctcca tattcctctg ccggccccag ccacctggat agtcatgatt
61 caagccagtt acttaatggc ctcaagacag cagctacaag cgtgtgggaa accagaatca
121 agctcttgtg ctgttgcatt gggaaagacg accatactcg ggttgctttt tcgagacaca
181 gatctggtgc ccagcgacat tgcggcgggc ctcgccctgc ttcatcagca acaggacaat
241 atcaggaaca accaagagcc tgcccaggtg gtctgccatg ccccagggag ctcccaggaa
301 gctgatctgg atgcagaatt agaaaactgc catcattaca tgcagtttgc agcagcggcc
361 tatgggtggc ccctctacat ctacagaaac cccctcacgg ggctgtgcag gattggtggt
421 gactgctgca gaagcagaac cacagactat gacttggtcg gaggcgatca gctcaactgt
481 cacttcggct ccatcctgca caccacaggg ctgcagtaca gggacttcat ccacgtcagc
541 ttccatgaca aggtttacga gctgccgttt ttagtggctc tggatcacag gaaagagtct
601 gttgtggtcg ctgtgagggg gaccatgtct ctgcaggatg tccttacgga cctgtcagcg
661 gagagtgagg tgctggacgt ggagtgtgag gtgcaggacc gcctggcaca caagggtatt
721 tctcaagctg ccagatacgt ttaccaacga ctcatcaacg acgggatttt gagccaagcc
781 ttcagcattg ctcctgagta ccggctggtc atagtgggcc acagcctcgg gggcggggcg
841 gccgccctgc tggccaccat gctcagagcc gcctacccgc aggtcaggtg ctacgccttc
901 tccccacccc gggggctgtg gagcaaagct ctgcaggaat attctcagag cttcatcgtg
961 tcactcgtcc tggggaagga tgtgattccc aggctcagtg tgaccaactt ggaagatctg
1021 aagagaagaa tcttgcgagt ggtcgcgcac tgcaataaac ccaagtacaa gatcttgctg
1081 cacggtttgt ggtacgaact gtttggagga aaccccaaca acttgcccac ggagctggac
1141 gggggcgacc aggaagtcct gacacagcct cttctggggg agcagagcct actgacgcgc
1201 tggtccccgg cctacagctt ctccagcgac tccccactgg actcttctcc caagtacccc
1261 cctctctacc ctcccggcag gatcatccac ctgcaggagg agggcgcctc ggggcggttt
1321 ggctgctgct ctgctgctca ctatagcgcc aagtggtcac acgaagcgga attcagcaaa
1381 atactcatag gtccgaagat gctcaccgac cacatgccag acatcctgat gcgggccttg
1441 gacagcgtgg tctccgacag agcggcctgc gtctcctgtc cagcacaagg ggtctccagt
1501 gtggacgtgg cctgaccagg gccactggaa actgtcccag gaacgatgga ctcacgcttt
1561 tgtccttaaa ctgacttacc atccgaggag ttcccatgac gccaaaacag cgaatgtcca
1621 tcaacaggaa tcggatggga acagaattcc atggtctcaa tgacttaagt ttatgggaag
1681 tcattgtggc cataatggta gcagaagtag tgagcacgct caggtgatag gacgactcct
1741 gagacccagc gaccgtggag acagcctcgg gaagccctgg cccgtggatg gatcccttgg
1801 ctgtctgagg actgctccag aagtgcggga atccagggcc cacccagaag accgtgaaca
1861 gttccttagc ctcccacccc caaggcagct cttttcatcc aactcagttt acaggcgtgg
1921 tttgtttttc aaactgggct tcctggatgt acaaatggaa ctgtggtgag ggtgcgggct
//