LOCUS AK298909 2019 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ53159 complete cds, highly similar to
Nucleoporin NDC1.
ACCESSION AK298909
VERSION AK298909.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2019)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2019
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2004807"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000493533"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 260..1939
/codon_start=1
/note="highly similar to Nucleoporin NDC1"
/protein_id="BAG61017.1"
/transl_table=1
/translation="MHSFIHAAMGMVMAWCAAVITQGQYSFLVVPCTGTNSFGSPAAQ
TCLNEYHLFFLLTGAFMGYSYSLLYFVNNMNYLPFPIIQQYKFLRFRRSLLLLVKHSC
VESLFLVRNFCILYYFLGYIPKAWISTAMNLHIDEQVHRPLDTVSGLLNLSLLYHVWL
CGVFLLTTWYVSWILFKIYATEAHVFPVQPPFAEGSDECLPKVLNSNPPPIIKYLALQ
DLMLLSQYSPSRRQEVFSLSQPGGHPHNWTAISRECLNLLNGMTQKLILYQEAAATNG
RVSSSYPVEPKKLNSPEETAFQTPKSSQMPRPSVPPLVKTSLFSSKLSTPDVVSPFGT
PFGSSVMNRMAGIFDVNTCYGSPQSPQLIRRGPRLWTSASDQQMTEFSNPSPSTSISA
EGKTMRQPSVIYSWIQNRREQIKNFLSKRVLIMYFFSKHPEASIQAVFSDAQMHIWAL
EGLSHLVAASFTEDRFGVVQTTLPAILNTLLTLQEAVDKYFKLPHASSKPPRISGSLV
DTSYKTLRFAFRASLKTAIYRITTTFGEHLNAVQASAEHQKRLQQFLEFKE"
BASE COUNT 541 a 459 c 407 g 612 t
ORIGIN
1 ctcctgtacg ccctagacta ggggccgcca tctccatggc cacggccgtg agccggccct
61 gcgccggcag gtcgcgggac atactgtggc gcattctttc agtgacctgt atagttccta
121 tgtaatcttt tacttcctgc tgctgtcagt ggtaataata ataataagta ttttcaatgt
181 ggagttctat gcagttgtgc cttctattcc ttgctccaga ctagctctga tagggaagat
241 cattcatcct cagcaactca tgcactcatt tattcatgct gcaatgggaa tggtgatggc
301 ctggtgtgct gcagtgataa cccagggcca gtacagcttt cttgtggttc cctgcactgg
361 tactaacagc tttggtagcc ctgctgcgca aacctgctta aatgaatatc atcttttttt
421 cctactgact ggagcattta tgggctatag ctatagcctc ctgtattttg ttaacaacat
481 gaactatctt ccatttccca tcatacagca atacaagttc ttgcgtttta ggagatctct
541 gctcttatta gttaaacaca gctgtgtgga atcactgttc ctggttagaa atttctgcat
601 tttatattat tttcttggct atattcccaa agcttggatt agcactgcta tgaaccttca
661 catagatgag caggttcata ggccacttga cacagtgagt ggcctcttaa atctctcgtt
721 actctaccat gtctggctgt gtggtgtctt tctcctgacg acttggtatg tctcatggat
781 actcttcaaa atctatgcca cagaggctca tgtgtttcct gttcaaccac catttgcaga
841 agggtcagat gagtgccttc caaaagtgtt aaatagcaat cctcccccca tcataaagta
901 tttagccttg caggacctga tgttgctttc tcaatattct ccttcacgaa gacaagaagt
961 tttcagcctc agccaaccag gtggacatcc ccacaattgg acagccattt caagggagtg
1021 tttgaatctt ttaaatggta tgactcagaa actgattctc tatcaagaag ctgctgctac
1081 gaatgggaga gtgtcttcat cttacccagt ggaacctaag aaattaaatt ctccagaaga
1141 aactgctttt cagacaccaa aatctagcca gatgcctcgg ccttcagtgc caccattagt
1201 taaaacatca ctattttctt caaaattatc tacacctgat gttgtgagcc catttgggac
1261 cccatttggc tctagtgtaa tgaatcggat ggctggaatt tttgatgtaa acacctgcta
1321 tgggtcaccg caaagtcctc agctaataag aagggggcca agattgtgga catcagcttc
1381 tgatcagcaa atgactgaat tttctaatcc ttctccatct acctctatta gtgctgaggg
1441 taagacaatg agacaaccca gtgtgattta ttcatggatt cagaatagac gtgaacagat
1501 taagaatttc ttgtcaaaac gggtgctgat aatgtatttt ttcagtaagc acccagaggc
1561 ctccattcag gctgtttttt cagatgccca aatgcatatt tgggcattag aaggtctgtc
1621 gcacttagta gcagcatcat ttacagagga tagatttgga gttgtccaga cgacactacc
1681 agctatcctt aatactttgt tgacactgca agaggcagtc gacaagtact ttaagcttcc
1741 tcatgcttcc agtaaaccac cccggatttc aggaagcctt gtggacactt catataaaac
1801 attaagattt gcattcagag catcactgaa aactgccatc tatcgaataa ctactacatt
1861 tggtgaacat ctgaatgctg tgcaagcatc tgcagaacat cagaaaagac ttcaacagtt
1921 cttggagttc aaagaatagt taagtaatat aaactgtgtt cattacactg ctgatacaac
1981 tacagatggg acagtaaatg ttcagcattc ttggatcag
//