LOCUS AK298833 2198 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ53475 complete cds, highly similar to
Autophagy-related protein 7.
ACCESSION AK298833
VERSION AK298833.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2198)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2198
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2NE2010547"
/clone_lib="NT2NE2"
/db_xref="H-InvDB:HIT000493457"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuron after the differentiation of
NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 62..2107
/codon_start=1
/note="highly similar to Autophagy-related protein 7"
/protein_id="BAG60963.1"
/transl_table=1
/translation="MAAATGDPGLSKLQFAPFSSALDVGFWHELTQKKLNEYRLDEAP
KDIKGYYYNGDSAGLPARLTLEFSAFDMSAPTPARCCPAIGTLYNTNTLESFKTADEN
PVLLNKFLLLTFADLKKYHFYYWFCYPALCLPESLPLIQGPVGLDQRFSLKQIEALEC
AYDNLCQTEGVTALPYFLIKYDENMVLVSLLKHYSDFFQGQRTKITIGVYDPCNLAQY
PGWPLRNFLVLAAHRWSSSFQSVEVVCFRDRTMQGARDVAHSIIFEVKLPEMAFSPDC
PKAVGWEKNQKGGMGPRMVNLSECMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVVSV
KCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISYSNPVRQPLYEFEDCLGGGKPKA
LAAADRLQKIFPGVNARGFNMSIPMPGHPVNFSSVTLEQARRDVEQLEQLIESHDVAF
LLMDTRESRWLPAVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPNHPVA
SADLLGSSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAV
ELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKC
TACSSKVLDQYEREGFNFLAKVFNSSHSFLEDLTGLTLLHQETQAAEIWDMSDDETI"
BASE COUNT 528 a 525 c 578 g 567 t
ORIGIN
1 atcggggcgc gcgcctcaga gagagctgtg gttgccggaa gttgagcggc ggcaagaaat
61 aatggcggca gctacggggg atcctggact ctctaaactg cagtttgccc cttttagtag
121 tgccttggat gttgggtttt ggcatgagtt gacccagaag aagctgaacg agtatcggct
181 ggatgaagct cccaaggaca ttaagggtta ttactacaat ggtgactctg ctgggctgcc
241 agctcgctta acattggagt tcagtgcttt tgacatgagt gctcccaccc cagcccgttg
301 ctgcccagct attggaacac tgtataacac caacacactc gagtctttca agactgcaga
361 tgaaaaccct gtactcctca acaagttcct cctcttgaca tttgcagatc taaagaagta
421 ccacttctac tattggtttt gctatcctgc cctctgtctt ccagagagtt tacctctcat
481 tcaggggcca gtgggtttgg atcaaaggtt ttcactaaaa cagattgaag cactagagtg
541 tgcatatgat aatctttgtc aaacagaagg agtcacagct cttccttact tcttaatcaa
601 gtatgatgag aacatggtgc tggtttcctt gcttaaacac tacagtgatt tcttccaagg
661 tcaaaggacg aagataacaa ttggtgtata tgatccctgt aacttagccc agtaccctgg
721 atggcctttg aggaattttt tggtcctagc agcccacaga tggagtagca gtttccagtc
781 tgttgaagtt gtttgcttcc gtgaccgtac catgcagggg gcgagagacg ttgcccacag
841 catcatcttc gaagtgaagc ttccagaaat ggcatttagc ccagattgtc ctaaagcagt
901 tggatgggaa aagaaccaga aaggaggcat gggaccaagg atggtgaacc tcagtgaatg
961 tatggaccct aaaaggttag ctgagtcatc agtggatcta aatctcaaac tgatgtgttg
1021 gagattggtt cctactttag acttggacaa ggttgtgtct gtcaaatgtc tgctgcttgg
1081 agccggcacc ttgggttgca atgtagctag gacgttgatg ggttggggcg tgagacacat
1141 cacatttgtg gacaatgcca agatctccta ctccaatcct gtgaggcagc ctctctatga
1201 gtttgaagat tgcctagggg gtggtaagcc caaggctctg gcagcagcgg accggctcca
1261 gaaaatattc cccggtgtga atgccagagg attcaacatg agcataccta tgcctgggca
1321 tccagtgaac ttctccagtg tcactctgga gcaagcccgc agagatgtgg agcaactgga
1381 gcagctcatc gaaagccatg atgtcgcctt cctattgatg gacaccaggg agagccggtg
1441 gcttcctgcc gtcattgctg caagcaagag aaagctggtc atcaatgctg ctttgggatt
1501 tgacacattt gttgtcatga gacatggtct gaagaaacca aagcagcaag gagctgggga
1561 cttgtgtcca aaccaccctg tggcatctgc tgacctcctg ggctcatcgc tttttgccaa
1621 catccctggt tacaagcttg gctgctactt ctgcaatgat gtggtggccc caggagattc
1681 aaccagagac cggaccttgg accagcagtg cactgtgagt cgtccaggac tggccgtgat
1741 tgcaggagcc ctggccgtgg aattgatggt atctgttttg cagcatccag aagggggcta
1801 tgccattgcc agcagcagtg acgatcggat gaatgagcct ccaacctctc ttgggcttgt
1861 gcctcaccag atccggggat ttctttcacg gtttgataat gtccttcccg tcagcctggc
1921 atttgacaaa tgtacagctt gttcttccaa agttcttgat caatatgaac gagaaggatt
1981 taacttccta gccaaggtgt ttaattcttc acattccttc ttagaagact tgactggtct
2041 tacattgctg catcaagaaa cccaagctgc tgagatctgg gacatgagcg acgatgagac
2101 catctgagat ggccccgctg tggggctgac ttctccccgg ccgcctgctg aggagctctc
2161 catcgccaga gcaggactgc tgaccccagg cctggtga
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