LOCUS AK298830 1959 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ52518 complete cds, highly similar to
6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44).
ACCESSION AK298830
VERSION AK298830.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1959)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1959
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2NE2009564"
/clone_lib="NT2NE2"
/db_xref="H-InvDB:HIT000493454"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuron after the differentiation of
NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 245..1657
/codon_start=1
/note="highly similar to 6-phosphogluconate
dehydrogenase, decarboxylating (EC 1.1.1.44)"
/protein_id="BAG60960.1"
/transl_table=1
/translation="MGQNLILNMNDHGFVVCAFNRTVSKVDDFLANEAKGTKVVGAQS
LKEMVSKLKKPRRIILLVKAGQAVDDFIEKLVPLLDTGDIIIDGGNSEYRDTTRRCRD
LKAKGILFVGSGVSGGEEGARYGPSLMPGGNKEAWPHIKTIFQGIAAKVGTGEPCCDW
VGDEGAGHFVKMVHNGIEYGDMQLICEAYHLMKDVLGMAQDEMAQAFEDWNKTELDSF
LIEITANILKFQDTDGKHLLPKIRDSAGQKGTGKWTAISALEYGVPVTLIGEAVFARC
LSSLKDERIQASKKLKGPQKFQFDGDKKSFLEDIRKALYASKIISYAQGFMLLRQAAT
EFGWTLNYGGIALMWRGGCIIRSVFLGKIKDAFDRNPELQNLLLDDFFKSAVENCQDS
WRRAVSTGVQAGIPMPCFTTALSFYDGYRHEMLPASLIQAQRDYFGAHTYELLAKPGQ
FIHTNWTGHGGTVSSSSYNA"
BASE COUNT 445 a 522 c 558 g 434 t
ORIGIN
1 atggcccagt gagtgactcg ccaggggcag cccggctcgg cctcagcggg cggggaactc
61 tttgggggtc gagatctccc tcgttctctc cgacgcctcc caccctgggg gtcgcctgag
121 ctcacttggg gctctgtgac cctggcccta cggcgtctcg ggcccagagc tccttccctg
181 cgggcccggc cccctgccct ctcggccgcg cagagctgac accgcgctga tcggattggc
241 cgtcatgggc cagaacttaa ttctgaacat gaatgaccac ggctttgtgg tctgtgcttt
301 taataggact gtctccaaag ttgatgattt cttggccaat gaggcaaagg gaaccaaagt
361 ggtgggtgcc cagtccctga aagagatggt ctccaagctg aagaagcccc ggcggatcat
421 cctcctggtg aaggctgggc aagctgtgga tgatttcatc gagaaattgg taccattgtt
481 ggatactggt gacatcatca ttgacggagg aaattctgaa tatagggaca ccacaagacg
541 gtgccgagac ctcaaggcca agggaatttt atttgtgggg agcggagtca gtggtggaga
601 ggaaggggcc cggtatggcc catcgctcat gccaggaggg aacaaagaag cgtggcccca
661 catcaagacc atcttccaag gcattgctgc aaaagtggga actggagaac cctgctgtga
721 ctgggtggga gatgagggag caggccactt cgtgaagatg gtgcacaacg ggatagagta
781 tggggacatg cagctgatct gtgaggcata ccacctgatg aaagacgtgc tgggcatggc
841 gcaggacgag atggcccagg cctttgagga ttggaataag acagagctag actcattcct
901 gattgaaatc acagccaata ttctcaagtt ccaagacacc gatggcaaac acctgctgcc
961 aaagatcagg gacagcgcgg ggcagaaggg cacagggaag tggaccgcca tctccgccct
1021 ggaatacggc gtacccgtca ccctcattgg agaagctgtc tttgctcggt gcttatcatc
1081 tctgaaggat gagagaattc aagctagcaa aaagctgaag ggtccccaga agttccagtt
1141 tgatggtgat aagaaatcat tcctggagga cattcggaag gcactctacg cttccaagat
1201 catctcttac gctcaaggct ttatgctgct aaggcaggcg gccaccgagt ttggctggac
1261 tctcaattat ggtggcatcg ccctgatgtg gagagggggc tgcatcatta gaagtgtatt
1321 cctaggaaag ataaaggatg catttgatcg aaacccggaa cttcagaacc tcctactgga
1381 cgacttcttt aagtcagctg ttgaaaactg ccaggactcc tggcggcggg cagtcagcac
1441 tggggtccag gctggcattc ccatgccctg ttttaccact gccctctcct tctatgacgg
1501 gtacagacat gagatgcttc cagccagcct catccaggct cagcgggatt acttcggggc
1561 tcacacctat gaactcttgg ccaaaccagg gcagtttatc cacaccaact ggacaggcca
1621 tggtggcacc gtgtcatcct cgtcatacaa tgcctgatca tgctgctcct gtcaccctcc
1681 acgattccac agaccaggac attccatgtg cctcatggca ctgccacctg gccctttgcc
1741 ctattttctg ttcagttttt taaaagtgtt gtaagagact cctgaggaag acacacagtt
1801 tatttgtaaa gtagctctgt gagagccacc atgccctctg cccttgcctc ttgggactga
1861 ccaggagctg ctcatgtgcg tgagagtggg aaccatctcc ttgcggcagt ggcttccgcg
1921 tgccccgtgt gctggtgcgg ttcccatcac gcagacagg
//