LOCUS AK298828 1943 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ51276 complete cds, highly similar to
Secretogranin-2 precursor.
ACCESSION AK298828
VERSION AK298828.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1943)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1943
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2NE2009322"
/clone_lib="NT2NE2"
/db_xref="H-InvDB:HIT000493452"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuron after the differentiation of
NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 91..1524
/codon_start=1
/note="highly similar to Secretogranin-2 precursor"
/protein_id="BAG60958.1"
/transl_table=1
/translation="MAEAKTHWLGAALSLIPLIFLISGAEAASFQRNQLLQKEPDLRL
ENVQKFPSPEMIRALEYIENLRQQAHKEESSPDYNPYQGVSVPLQQKENGDESHLPER
DSLSGEDWNPVEEKIESQTQEEVRDSKENIEKNEQINDEMKRSGQLGIQEEDLRKESK
DQLSDDVSKVIAYLKRLVNAAGSGRLQNGQNGERATRLFEKPLDSQSIYQLIEISRNL
QIPPEDLIEMLKTGEKPNGSVEPERELDLPVDLDDISEADLDHPDLFQNRMLSKSGYP
KTPGRAGTEALPDGLSVEDILNLLGMESAANQKTSYFPNPYNQEKVLPRLPYGAGRSR
SNQLPKAAWIPHVENRQMAYENLNDKDQELGEYLARMLVKYPEIINSNQVKRVPGQGS
SEDDLQEEEQIEQAIKEHLNQGSSQETDKLAPVSKRFPVGPPKNDDTPNRQYWDEDLL
MKVLEYLNQEKAEKGREHIAKRAMENM"
BASE COUNT 631 a 389 c 451 g 472 t
ORIGIN
1 aaacggcccg agaagctcgc ccggagaacg gggaggaata tgctgtggag ctcctctgcc
61 atataaacaa aaagaggaaa tctttcaaac atggctgaag caaagaccca ctggcttgga
121 gcagccctgt ctcttatccc tttaattttc ctcatctctg gggctgaagc agcttcattt
181 cagagaaacc agctgcttca gaaagaacca gacctcaggt tggaaaatgt ccaaaagttt
241 cccagtcctg aaatgatcag ggctttggag tacatagaaa acctccgaca acaagctcat
301 aaggaagaaa gcagcccaga ttataatccc taccaaggtg tctctgtccc ccttcagcaa
361 aaagaaaatg gcgatgaaag ccacttgccc gagagggatt cactgagtgg agaagactgg
421 aacccagtag aggagaaaat agagagtcaa acccaggaag aggtgagaga cagcaaagag
481 aatatagaaa aaaatgaaca aatcaacgat gagatgaaac gctcagggca gcttggcatc
541 caggaagaag atcttcggaa agagagtaaa gaccaactct cagatgatgt ctccaaagta
601 attgcctatt tgaaaaggtt agtaaatgct gcaggaagtg ggaggttaca gaatgggcaa
661 aatggggaaa gggccaccag gctttttgag aaacctcttg attctcagtc tatttatcag
721 ctgattgaaa tctcaaggaa tttacagata cccccagaag acttaattga gatgctcaaa
781 actggggaga agccgaatgg atcagtggaa ccggagcggg agcttgacct tcctgttgac
841 ctagatgaca tctcagaggc tgacttagac catccagacc tgttccaaaa taggatgctc
901 tccaagagtg gctaccctaa aacacctggt cgtgctggga ctgaggccct accagacggg
961 ctcagtgttg aggatatttt aaatctttta gggatggaga gtgcagcaaa tcagaaaacg
1021 tcgtattttc ccaatccata taaccaggag aaagttctgc caaggctccc ttatggtgct
1081 ggaagatcta gatcgaacca gcttcccaaa gctgcctgga ttccacatgt tgaaaacaga
1141 cagatggcat atgaaaacct gaacgacaag gatcaagaat taggtgagta cttggccagg
1201 atgctagtta aataccctga gatcattaat tcaaaccaag tgaagcgagt tcctggtcaa
1261 ggctcatctg aagatgacct gcaggaagag gaacaaattg agcaggccat caaagagcat
1321 ttgaatcaag gcagctctca ggagactgac aagctggccc cggtgagcaa aaggttccct
1381 gtggggcccc cgaagaatga tgatacccca aataggcagt actgggatga agatctgtta
1441 atgaaagtgc tggaatacct caaccaagaa aaggcagaaa agggaaggga gcatattgct
1501 aagagagcaa tggaaaatat gtaagctgct ttcattaatt accctacttt cattcctccc
1561 accccaagca aatcccaaca tttctcttca gtgtgttgac ttctatcctg ttaacactgt
1621 aatatcttta aatgatgtac aggcagatga aaccaggtca ctggggagtc tgcttcattt
1681 cctctgagct gttatcttgt gtatggatat gtgtaaatgt tatgactcct tgataaaaaa
1741 tttattatgt ccattattca agaaagatat ctatgactgt gtttaatagt atatctaatg
1801 gctgtggcat tgttgatgct cacatatgat aaaaaagtgt cctataattc tattgaaagt
1861 ttttaatatt tattgaatta ttttgttact gtctgtagtg ttttgtggag tactggacca
1921 aaaaaataaa gcattataaa tat
//