LOCUS AK298801 1774 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ59382 complete cds, highly similar to
T-complex protein 1 subunit theta.
ACCESSION AK298801
VERSION AK298801.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1774)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1774
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2NE2004925"
/clone_lib="NT2NE2"
/db_xref="H-InvDB:HIT000493425"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuron after the differentiation of
NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 201..1628
/codon_start=1
/note="highly similar to T-complex protein 1 subunit
theta"
/protein_id="BAG60936.1"
/transl_table=1
/translation="MDQMVQHPAAKMIVMASHMQEQEVGDGTNFVLVFAGALLELAEE
LLRIGLSVSEVIEGYEIACRKAHEILPNLVCCSAKNLRDIDEVSSLLRTSIMSKQYGN
EVFLAKLIAQACVSIFPDSGHFNVDNIRVCKILGSGISSSSVLHGMVFKKETEGDVTS
VKDAKIAVYSCPFDGMITETKGTVLIKTAEELMNFSKGEENLMDAQVKAIADTGANVV
VTGGKVADMALHYANKYNIMLVRLNSKWDLRRLCKTVGATALPRLTPPVLEEMGHCDS
VYLSEVGDTQVVVFKHEKEDGAISTIVLRGSTDNLMDDIERAVDDGVNTFKVLTRDKR
LVPGGGATEIELAKQITSYGETCPGLEQYAIKKFAEAFEAIPRALAENSGVKANEVIS
KLYAVHQEGNKNVGLDIEAEVPAVKDMLEAGILDTYLGKYWAIKLATNAAVTVLRVDQ
IIMAKPAGGPKPPSGKKDWDDDQND"
BASE COUNT 525 a 326 c 417 g 506 t
ORIGIN
1 gctcctcgct ttctccgcgg tcttccgagc ggtcgcgtga actgcttcct gcaggctggc
61 catggcgctt cacgttccca aggctccggg ctttgcccag atgctcaagg agggagcgaa
121 acacttttca ggattagaag aggctgtgta tagaaacata caagcttgca aggagcttgc
181 ccaaaccact cgtacagcat atggaccaaa tggtacagca tcctgctgca aaaatgattg
241 taatggcttc tcatatgcaa gagcaagaag ttggagatgg cacaaacttt gttctggtat
301 ttgctggagc tctcctggaa ttagctgaag aacttctgag gattggcctg tcagtttcag
361 aggtcataga aggttatgaa atagcctgca gaaaagctca tgagattctt cctaatttgg
421 tatgttgttc tgcaaaaaac cttcgagata ttgatgaagt ctcatctcta cttcgtacct
481 ccataatgag taaacaatat ggtaatgaag tatttctggc caagcttatt gctcaggcat
541 gcgtatctat ttttcctgat tccggccatt tcaatgttga taacatcaga gtttgtaaaa
601 ttctgggctc tggtatcagt tcctcttcag tattgcatgg catggttttt aagaaggaaa
661 ccgaaggtga tgtaacatct gtcaaagatg caaaaatagc agtgtactct tgtccttttg
721 atggcatgat aacagaaact aagggaacag tgttgataaa gactgctgaa gaattgatga
781 attttagtaa gggagaagaa aacctcatgg atgcacaagt caaagctatt gctgatactg
841 gtgcaaatgt cgtagtaaca ggtggcaaag tggcagacat ggctcttcat tatgcaaata
901 aatataatat catgttagtg aggctaaact caaaatggga tctccgaaga ctttgtaaaa
961 ctgttggtgc tacagctctt cctagattga cacctcctgt ccttgaagaa atgggacact
1021 gtgacagtgt ttacctctca gaagttggag atactcaggt ggtggttttt aagcatgaaa
1081 aggaagatgg cgccatttct accatagtac ttcgaggctc tacagacaat ctgatggatg
1141 acatagaaag ggcagtagac gatggtgtta atactttcaa agttcttaca agggataaac
1201 gtcttgtacc cggaggtgga gcaacagaaa ttgaattagc caaacagatc acatcatatg
1261 gagagacatg tcctggactt gaacagtatg ctattaagaa gtttgctgag gcatttgaag
1321 ctattccccg cgcactggca gaaaactctg gagttaaggc caatgaagta atctctaaac
1381 tttatgcagt acatcaagaa ggaaataaaa acgttggatt agatattgag gctgaagtcc
1441 ctgctgtaaa ggacatgctg gaagctggta ttctagatac ttacctggga aaatattggg
1501 ctatcaaact cgctactaat gctgcagtca ctgtacttag agtggatcag atcatcatgg
1561 caaaaccagc tggtgggccc aagcctccaa gtgggaagaa agactgggat gatgaccaaa
1621 atgattgaaa ttggcttaat ttttactgta ggtgaaggct gtatttgtag tagtactcaa
1681 gaatcacctg atgttttctt attctcctta aattaagagt tattttgtgt ttgtattctt
1741 ggctggatgt tataataaac atattgttac tgtc
//