LOCUS AK298786 2224 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ50613 complete cds, highly similar to
Vacuolar protein sorting-associated protein 45.
ACCESSION AK298786
VERSION AK298786.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2224)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2224
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2NE2002723"
/clone_lib="NT2NE2"
/db_xref="H-InvDB:HIT000493410"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuron after the differentiation of
NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 241..1857
/codon_start=1
/note="highly similar to Vacuolar protein
sorting-associated protein 45"
/protein_id="BAH12870.1"
/transl_table=1
/translation="MVYTQSEILQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVD
YIIQELRRPKYTIYFIYFSNVISKSDVKSLAEADEQEVVAEVQQVITKEYELFEFRRT
EVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLS
AENDEFYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKK
MSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQNDHSSALQNIKRLLQNPKVTE
FDAARLVMLYALHYERHSSNCLPGLMMDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDL
FSPKDAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKGNLYPYLGPSTLRD
RPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTVHNTKRDGVSLCSPAWFRT
PGLKRSTRLSLPKCWDYSFPRGSSGFWTAQPKQGELSSHIKVSEQKMKRWLGEGHSFL
SCPHYRFSLLNKGVGEQLWVLCWLLELISR"
BASE COUNT 630 a 510 c 507 g 577 t
ORIGIN
1 atgccacctc ccttgccctc atctctcact gccatgcgag aaagcgttaa tggcaaatag
61 ggcacgtagc cacactccgt cgttcctgct tccaagcaaa ccttgtgcca ggttcctcat
121 ttcgcgaggc tccactctct tgggcttcag gctgccgctg ggcctccagc accaaagggc
181 aataggggtc tgagagtccg ggcctcaggc atcttcaggc cgcagactgg catagtgagt
241 atggtataca cacaatcgga gattctacag aaggaagtgt acctctttga acgcattgat
301 tctcaaaatc gagagatcat gaaacacctg aaggcaattt gttttcttcg acctacaaag
361 gagaatgtgg attatattat tcaggagctc cgaagaccca aatacactat atatttcatt
421 tatttcagta atgtgatcag caagagtgac gtgaagtcat tggctgaagc tgatgaacag
481 gaagttgtgg ctgaggttca gcaagtgata actaaagaat atgaactgtt tgaattccgt
541 cggacagagg ttcctccatt gctccttatt ttagatcgct gtgatgatgc catcacccca
601 ttgctaaacc agtggacata tcaggccatg gtccacgaac tactaggcat aaacaacaat
661 cggattgatc tttccagagt gccgggaatc agtaaagact taagagaagt ggtcctatct
721 gctgaaaatg atgaattcta tgctaataat atgtacctga actttgctga gattggtagc
781 aatataaaga atctcatgga agattttcag aagaagaaac caaaagaaca gcaaaaacta
841 gaatcaatag cagacatgaa ggcgtttgtt gagaattatc cacagttcaa gaaaatgtct
901 gggactgttt caaagcatgt gacagtggtt ggagaactgt ctcgattggt cagtgaacgg
961 aatctgctgg aggtttcaga ggttgagcaa gaactggcct gtcaaaatga ccattctagt
1021 gctctccaga atataaaaag gcttctgcag aaccccaaag tgacagagtt tgatgctgcc
1081 cgcctggtga tgctttatgc tttacattat gagcgacaca gcagcaattg cctgccagga
1141 ctaatgatgg acctcaggaa taaaggtgtt tctgagaagt atcgaaagct cgtgtctgca
1201 gttgttgaat atggtggtaa acgagtcaga ggaagtgacc tcttcagccc caaagatgct
1261 gtggctatca ccaaacaatt cctcaaagga ctgaagggag tagaaaatgt atatacacag
1321 catcaacctt tcctacatga aaccctggat catctcatca aaggaaggct taagggaaac
1381 ctatatcctt atttaggccc cagcacactc agagacagac ctcaggatat cattgtgttt
1441 gtaattggag gagccaccta tgaagaggct ctaacagttt ataacctgaa ccgcaccact
1501 cctggagtga ggattgtcct gggaggcacc acagtgcaca acacgaaaag agatggggtt
1561 tcactatgtt cccctgcctg gtttcgaact cctgggctca agcgatccac ccgcctcagc
1621 ctcccaaagt gctgggatta cagctttcct agaggaagtt ctggcttctg gactgcacag
1681 ccgaagcaag gagagctctc aagtcacatc aaggtcagcg agcagaagat gaaacggtgg
1741 ttgggggaag ggcacagctt cctctcttgt ccccactaca ggttttccct actaaacaaa
1801 ggtgttggag agcagctttg ggttctgtgc tggttgttag aactcatctc caggtagccc
1861 acggatacgt ggttggcaca gacacaagac tcccagagtt gtcctaacaa taagtctgag
1921 cccatctcaa cccacttttc tccggtagtc tttatgtatc tgttagcaca atcacttcag
1981 ttactgatga atcttgttgg gatctgactt ggggaaaggg ttatcagagc ctagaggggc
2041 ttaaaaagta atcatttgat gtacatacca cactccttgg cttcctttct cttcccttaa
2101 ccctttctgc ttttcattaa ccacattcct gcacaactca tttctgaaaa cctaccatgt
2161 ttctttacag agccatccaa aaattttttg tccctacata gcaattttct gtggcactga
2221 gaaa
//