LOCUS AK298732 1643 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ58271 complete cds, highly similar to
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4.
ACCESSION AK298732
VERSION AK298732.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1643)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1643
/cell_type="neutrophils"
/clone="NETRP2008168"
/clone_lib="NETRP2"
/db_xref="H-InvDB:HIT000493356"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, neutrophils"
/organism="Homo sapiens"
CDS 15..1391
/codon_start=1
/note="highly similar to
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4"
/protein_id="BAH12859.1"
/transl_table=1
/translation="MDRGSLGGSSLTDLGPGSSRRVCMTNCPTLIVMVGLPARGKTYI
SKKLTRYLNWIGVPTREFNVGQYRRDVVKTYKSFEFFLPDNEEGLKIRKQCALAALRD
VRRFLSEEGGHVAVFDATNTTRERRATIFNFGEQNGYKTFFVESICVDPEVIAANIVQ
VKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYVVNR
VADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFAKSLAQ
FISDQNIKDLKVWTSQMKRTIQTAEALGVPYEQWKVLNEIDAGVCEEMTYEEIQDNYP
LEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFL
DKAAEQLPYLKCPLHTVLKLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPPEEA
LVTVPAHQ"
BASE COUNT 379 a 444 c 482 g 338 t
ORIGIN
1 agtttggttg caggatggac agaggctcgt taggaggcag ctccctgacc gacctggggc
61 cggggagcag caggcgggtg tgcatgacca actgcccaac tctcattgtc atggtgggcc
121 tgcccgccag gggcaagacc tacatctcca agaagctgac tcgatacctg aactggattg
181 gtgtgcccac tcgggagttc aatgttggcc agtatcgccg ggacgtggtc aagacctaca
241 aatcttttga attttttctc cccgacaatg aagagggcct gaaaatcagg aagcagtgtg
301 ccctggcagc cctccgtgac gtccggcggt tccttagtga ggagggggga catgtggcgg
361 tttttgatgc cacaaacacc acccgagaac ggagagcgac catctttaat tttggagaac
421 agaatggcta caagaccttt tttgtcgagt ccatctgtgt ggatcctgag gtcatagctg
481 ccaacatcgt gcaagtgaaa ctgggcagcc ctgactatgt caaccgcgac agtgatgagg
541 ctacggagga cttcatgagg cgcattgagt gctatgagaa ctcctacgag tcgctagatg
601 aggacctgga tagggacctg tcctatatca agatcatgga tgtgggccag agctacgtgg
661 tgaaccgtgt ggctgaccac atccagagcc gcatcgtata ttacctcatg aacatccacg
721 tgaccccccg ctccatctac ctctgccggc acggggagag cgagctcaac ctcaagggcc
781 ggattggcgg ggacccagga ctgtcccctc ggggcaggga gtttgccaag agtctagccc
841 agttcatcag tgaccaaaat atcaaggatc tgaaggtctg gacaagccag atgaagagga
901 caatccagac ggctgaggca ctgggtgtgc cctatgaaca gtggaaggtc ctcaacgaga
961 tcgatgcggg cgtctgtgag gaaatgacct acgaggaaat tcaggataat tatccactgg
1021 agttcgccct gcgggaccag gacaagtacc ggtaccggta ccctaaaggg gagtcctacg
1081 aggacctggt ccagagactg gagcctgtca tcatggagct ggagaggcaa gagaatgtgc
1141 tggtcatctg ccaccaggct gtgatgcgct gcctgctggc ctacttcctc gacaaggcag
1201 cagaacagct gccctacctc aagtgtccgc tgcacacagt cctgaagctg actcctgtgg
1261 catatggttg taaagtggag tccatattcc tgaacgtggc tgctgtgaac acgcaccggg
1321 acaggcctca gaacgtggac atctcaagac ctccagagga agcccttgtc acggtgcctg
1381 ctcaccagtg accatgttca tccactgtga ccactaggca ggcactgctc tctgcagagg
1441 gggtcattcc aggccctcca gtgtgtgtga tagtcaccat gccatgcagg gatattcttg
1501 aagccacaca tggctggcgg aacccagagc ccccacccca gcccacctgg ctctttgttg
1561 acagtcggcg acaaggttgt gcgtggctcc tgacctgctg ctaagagtca cttgaccaga
1621 ctgcatctgc atgggctgcg cgg
//