LOCUS AK298609 2342 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ53156 complete cds, highly similar to Homo
sapiens sphingomyelin phosphodiesterase 4, neutral membrane,
transcript variant 2, mRNA.
ACCESSION AK298609
VERSION AK298609.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2342)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2342
/cell_type="mesenchymal stem cells"
/clone="N1ESE2001548"
/clone_lib="N1ESE2"
/db_xref="H-InvDB:HIT000493233"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 240..2063
/codon_start=1
/note="highly similar to Homo sapiens sphingomyelin
phosphodiesterase 4, neutral membrane, transcript variant
2, mRNA"
/protein_id="BAG60793.1"
/transl_table=1
/translation="MQTPPPTRSGGQKLCSRFLLKCGFITIPWRCIKKCSPLMPSWRF
CTTDSVSPAPSTAPPNPASRPSTPTKSLGNRGSDARGFYWAEFSSGCVQDEEQESFTP
TEEHVLVVRLLLKHLHAFANSLKPEQASPSAHSHATSPLEEFKRAAVPRFVQQKLYLF
LQHCFGHWPLDASFRAVLEMWLSYLQPWRYAPDKQAPGSDSQPRCVSEKWAPFVQENL
LMYTKLFVGFLNRALRTDLVSPKHALMVFRVAKVFAQPNLAEMIQKGEQLFLEPELVI
PHRQHRLFTAPTFTGSFLSPWPPAVTDASFKVKSHVYSLEGQDCKYTPMFGPEARTLV
LRLAQLITQAKHTAKSISDQCAESPAGHSFLSWLGFSSMDTNGSYTANDLDEMGQDSV
RKTDEYLEKALEYLRQIFRLSEAQLRQFTLALGTTQDENGKKQLPDCIVGEDGLILTP
LGRYQIINGLRRFEIEYQGDPELQPIRSYEIASLVRTLFRLSSAINHRFAGQMAALCS
RDDFLGSFCRYHLTEPGLASRHLLSPVGRRQVAGHTRGPRLSLRFLGSYRTLVSLLLA
FFVASLFCVGPLPCTLLLTLGYVLYASAMTLLTERGKLHQP"
BASE COUNT 462 a 776 c 651 g 453 t
ORIGIN
1 atgacgactt tcggcgccgt ggcggaatgg cggcttccat ctctgaggcg agcgacgcta
61 tggatcccac agtggtttgc taagaaggcc attttcaact ctccactgga ggctgctatg
121 gcgttccctc acctgcagca gcccagcttt ctactggaca ccagccatac cctttgcttc
181 ctatggcctc caccacacta gcctcctaaa gcgacacatc tctcatcaga catctgtgaa
241 tgcagacccc gcctcccacg agatctggag gtcagaaact ctgctccagg tttttgttga
301 aatgtggctt catcactatt ccttggagat gtatcaaaaa atgcagtccc ctcatgccaa
361 gctggaggtt ctgcactacc gactcagtgt ctccagcgcc ctctacagcc ccgcccaacc
421 cagcctccag gccctccacg cctaccaagt ccctgggcaa cagaggaagt gacgcccggg
481 gcttctactg ggccgagttc tcatctggct gtgtccagga tgaggagcag gagtcgttca
541 cgcctactga ggagcatgtg ttggtggtgc gcctgctgct gaagcacctg cacgcctttg
601 ccaacagcct gaagccagag caggcctcac cctccgccca ctcccacgcc accagccccc
661 tggaggagtt caaacgggct gctgtcccga ggttcgtcca gcagaaactc tacctcttct
721 tgcagcattg ctttggccac tggcccctgg acgcatcgtt cagagctgtc ctggagatgt
781 ggctgagcta cctgcagccg tggcggtacg cgcctgacaa gcaggctccg ggcagcgact
841 cccagccccg gtgtgtgtcg gagaaatggg caccctttgt ccaggagaac ctgctgatgt
901 acaccaagtt gtttgtgggc tttctgaacc gcgcgctccg cacagacctg gtcagcccca
961 agcacgcgct catggtgttc cgagtggcca aagtctttgc ccagcccaac ctggctgaga
1021 tgattcagaa aggtgagcag ctattcctgg agccagagct ggtcatcccc caccgccagc
1081 accgactctt cacggccccc acattcactg ggagcttcct gtcaccctgg ccaccagcgg
1141 tcactgatgc ctccttcaag gtgaagagcc acgtctacag cctggagggc caggactgca
1201 agtacacccc gatgtttggg cccgaggccc gcaccctggt cctgcgcctc gctcagctca
1261 tcacacaggc caaacacaca gccaagtcca tctccgacca gtgtgcggag agcccggctg
1321 gccactcctt cctctcatgg ctgggcttta gctccatgga caccaatggc tcctacacag
1381 ccaacgacct ggacgagatg gggcaagaca gtgtccggaa gacagatgaa tacctggaga
1441 aggccctgga gtacctgcgc cagatattcc ggctcagcga agcgcagctc aggcagttca
1501 cactcgcctt gggcaccacc caggatgaga atggaaaaaa gcaactcccc gactgcatcg
1561 tgggtgagga cggactcatc cttacgcccc tggggcggta ccagatcatc aatgggctgc
1621 gaaggtttga aattgagtac cagggggacc cggagctgca gcccatccgg agctatgaga
1681 tcgccagctt ggtccgcaca ctctttaggc tgtcgtctgc catcaaccac agatttgcag
1741 gacagatggc ggctctgtgt tcccgggatg acttcctcgg cagcttctgt cgctaccacc
1801 tcacagaacc tgggctggcc agcaggcacc tgctgagccc tgtggggcgg aggcaggtgg
1861 ccggccacac ccgcggcccc aggctcagcc tgcgcttcct gggcagttac cggacgctgg
1921 tctcgctgct gctggccttc ttcgtggcct ctctgttctg cgtcgggccc ctcccatgca
1981 cgctgctgct caccctgggc tatgtcctct acgcctctgc catgacactg ctgaccgagc
2041 gggggaagct gcaccagccc tgaaggtgtc agctgccttc agagcaggct ggagggattt
2101 gccacacagc cccacccttg ggctgagagg acctgggaag cccctccagg agggaacacg
2161 gtcatcctcg ggcttctgga gcggggttcc tgcagccgca gaggcatctg gaggaaacgc
2221 aaccaagaaa ggaaggcagg tgggccccag caaaggagta gctgccaggg ctcaacagct
2281 acgctctgtg acagcgcaga gctcagcgcc ggcctttccc tccctccgcc aaggactcac
2341 gg
//