LOCUS AK298583 2428 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ50608 complete cds, highly similar to DNA
mismatch repair protein Mlh1.
ACCESSION AK298583
VERSION AK298583.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2428)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2428
/cell_type="normal mesangial cells (NHMC56046-2)"
/clone="MESAN2016903"
/clone_lib="MESAN2"
/db_xref="H-InvDB:HIT000493207"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal mesangial cells"
/organism="Homo sapiens"
CDS 261..2237
/codon_start=1
/note="highly similar to DNA mismatch repair protein Mlh1"
/protein_id="BAG60773.1"
/transl_table=1
/translation="MAFEALASISHVAHVTITTKTADGKCAYRASYSDGKLKAPPKPC
AGNQGTQITVEDLFYNIATRRKALKNPSEEYGKILEVVGRYSVHNAGISFSVKKQGET
VADVRTLPNASTVDNIRSIFGNAVSRELIEIGCEDKTLAFKMNGYISNANYSVKKCIF
LLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHF
LHEESILERVQQHIESKLLGSNSSRMYFTQTLLPGHAGPSGEMVKSTTSLTSSSTSGS
SDKVYAHQMVRTDSREQKLDAFLQPLSKPLSSQPQAIVTEDKTDISSGRARQQDEEML
ELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSSNPRKRHREDSDVEMVEDDSRKEM
TAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQWALAQHQTKLY
LLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEG
LAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILRLATEV
NWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPGSIPNSWKWTVEHIVYK
ALRSHILPPKHFTEDGNILQLANLPDLYKVFERC"
BASE COUNT 700 a 531 c 565 g 632 t
ORIGIN
1 gacgtgccag tcaggccttc tccttttccg cagaccgtgt gtttctttac cgctctcccc
61 cgagaccttt taagggttgt ttggagtttt agatgcaaaa tccacaagta ttcaagtgat
121 tgttaaagag ggaggcctga agttgattca gatccaagac aatggcaccg ggatcaggaa
181 agaagatctg gatattgtat gtgaaaggtt cactactagt aaactgcagt cctttgagga
241 tttagccagt atttctacct atggctttcg aggctttggc cagcataagc catgtggctc
301 atgttactat tacaacgaaa acagctgatg gaaagtgtgc atacagagca agttactcag
361 atggaaaact gaaagcccct cctaaaccat gtgctggcaa tcaagggacc cagatcacgg
421 tggaggacct tttttacaac atagccacga ggagaaaagc tttaaaaaat ccaagtgaag
481 aatatgggaa aattttggaa gttgttggca ggtattcagt acacaatgca ggcattagtt
541 tctcagttaa aaaacaagga gagacagtag ctgatgttag gacactaccc aatgcctcaa
601 ccgtggacaa tattcgctcc atctttggaa atgctgttag tcgagaactg atagaaattg
661 gatgtgagga taaaacccta gccttcaaaa tgaatggtta catatccaat gcaaactact
721 cagtgaagaa gtgcatcttc ttactcttca tcaaccatcg tctggtagaa tcaacttcct
781 tgagaaaagc catagaaaca gtgtatgcag cctatttgcc caaaaacaca cacccattcc
841 tgtacctcag tttagaaatc agtccccaga atgtggatgt taatgtgcac cccacaaagc
901 atgaagttca cttcctgcac gaggagagca tcctggagcg ggtgcagcag cacatcgaga
961 gcaagctcct gggctccaat tcctccagga tgtacttcac ccagactttg ctaccaggac
1021 atgctggccc ctctggggag atggttaaat ccacaacaag tctgacctcg tcttctactt
1081 ctggaagtag tgataaggtc tatgcccacc agatggttcg tacagattcc cgggaacaga
1141 agcttgatgc atttctgcag cctctgagca aacccctgtc cagtcagccc caggccattg
1201 tcacagagga taagacagat atttctagtg gcagggctag gcagcaagat gaggagatgc
1261 ttgaactccc agcccctgct gaagtggctg ccaaaaatca gagcttggag ggggatacaa
1321 caaaggggac ttcagaaatg tcagagaaga gaggacctac ttccagcaac cccagaaaga
1381 gacatcggga agattctgat gtggaaatgg tggaagatga ttcccgaaag gaaatgactg
1441 cagcttgtac cccccggaga aggatcatta acctcactag tgttttgagt ctccaggaag
1501 aaattaatga gcagggacat gaggttctcc gggagatgtt gcataaccac tccttcgtgg
1561 gctgtgtgaa tcctcagtgg gccttggcac agcatcaaac caagttatac cttctcaaca
1621 ccaccaagct tagtgaagaa ctgttctacc agatactcat ttatgatttt gccaattttg
1681 gtgttctcag gttatcggag ccagcaccgc tctttgacct tgccatgctt gccttagata
1741 gtccagagag tggctggaca gaggaagatg gtcccaaaga aggacttgct gaatacattg
1801 ttgagtttct gaagaagaag gctgagatgc ttgcagacta tttctctttg gaaattgatg
1861 aggaagggaa cctgattgga ttaccccttc tgattgacaa ctatgtgccc cctttggagg
1921 gactgcctat cttcattctt cgactagcca ctgaggtgaa ttgggacgaa gaaaaggaat
1981 gttttgaaag cctcagtaaa gaatgcgcta tgttctattc catccggaag cagtacatat
2041 ctgaggagtc gaccctctca ggccagcaga gtgaagtgcc tggctccatt ccaaactcct
2101 ggaagtggac tgtggaacac attgtctata aagccttgcg ctcacacatt ctgcctccta
2161 aacatttcac agaagatgga aatatcctgc agcttgctaa cctgcctgat ctatacaaag
2221 tctttgagag gtgttaaata tggttattta tgcactgtgg gatgtgttct tctttctctg
2281 tattccgata caaagtgttg tatcaaagtg tgatatacaa agtgtaccaa cataagtgtt
2341 ggtagcactt aagacttata cttgccttct gatagtattc ctttatacac agtggattga
2401 ttataaataa atagatgtgt cttaacat
//