LOCUS AK298565 2208 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ56379 complete cds, highly similar to Mus
musculus nexilin (Nexn), mRNA.
ACCESSION AK298565
VERSION AK298565.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2208)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2208
/cell_type="normal mesangial cells (NHMC56046-2)"
/clone="MESAN2012760"
/clone_lib="MESAN2"
/db_xref="H-InvDB:HIT000493189"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal mesangial cells"
/organism="Homo sapiens"
CDS 267..2102
/codon_start=1
/note="highly similar to Mus musculus nexilin (Nexn),
mRNA"
/protein_id="BAG60759.1"
/transl_table=1
/translation="MNDISQKAEIKEMLASDDEEDVSSKVEKAYVPKLTGTVKGRFAE
MEKQRQEEQRKRTEEERKRRIEQDMLEKRKIQRELAKRAEQIEDINNTGTESASEEGD
DSLLITVVPVKSYKTSGKMKKNFEDLEKEREEKERIKYEEDKRIRYEEQRPSLKEAKC
LSLVMDDEIESEAKKESLSPGKLKLTFEELERQRQENRKKQAEEEARKRLEEEKRAFE
EARRQMVNEDEENQDTAKIFKGYRPGKLKLSFEEMERQRREDEKRKAEEEARRRIEEE
KKAFAEARRNMVVDDDSPEMYKTISQEFLTPGKLEINFEELLKQKMEEEKRRTEEERK
HKLEMEKQEFEQLRQEMGEEEEENETFGLSREYEELIKLKRSGSIQAKNLKSKFEKIG
QLSEKEIQKKIEEERARRRAIDLEIKEREAENFHEEDDVDVRPARKSEAPFTHKVNMK
ARFEQMAKAREEEEQRRIEEQKLLRMQFEQREIDAALQKKREEEEEEEGSIMNGSTAE
DEEQTRSGAPWFKKPLKNTSVVDSEPVRFTVKVTGEPKPEITWWFEGEILQDGEDYQY
IERGETYCLYLPETFPEDGGEYMCKAVNNKGSAASTCILTIESKN"
BASE COUNT 888 a 349 c 539 g 432 t
ORIGIN
1 aatgcgggaa caggctgcac accaggactt ttatggaaac ttgctgctgg agacggcggc
61 ggcggcggcg gcagcggcag ccagaggact cccagcggct ggagcagaag tgttagcggc
121 cagagctccc agacccctac ccacagccag gcgggacgcg cacagtccct ccacgcggaa
181 agaagtacct tcgccggtca ccggctcctg cagggtgcaa atatatacag agcttcataa
241 tcagcccaag accacataga gcaaacatga atgatatttc ccaaaaggct gagattaaag
301 aaatgcttgc ttctgatgat gaggaagatg tatcttctaa agtagaaaag gcttatgttc
361 caaaattaac aggaactgtg aagggtagat ttgctgaaat ggagaaacaa agacaagagg
421 aacaaaggaa gagaacggag gaggaacgaa aacgcagaat tgagcaggat atgttagaaa
481 agaggaaaat acagcgtgaa ttagcaaaaa gggctgaaca gattgaggac ataaacaata
541 cgggaactga atcagcatca gaggaaggag atgattcact acttataact gtggtacctg
601 tcaaatcata taaaacatct ggaaaaatga aaaagaattt tgaggatcta gaaaaagaac
661 gtgaagagaa agaaaggatc aagtacgagg aagataaaag aataagatat gaagaacaac
721 gaccatctct caaggaagca aagtgtcttt cattagttat ggatgatgaa atagaaagtg
781 aagcaaaaaa agaatcactt tctcccggaa aattgaaact aacttttgaa gaactggagc
841 gacaaagaca agaaaaccga aagaagcaag ctgaagagga agcaagaaaa cgtttagaag
901 aagagaagcg tgcttttgaa gaagcaaggc ggcaaatggt aaatgaagat gaggaaaacc
961 aagacacagc aaaaattttt aaagggtacc gccctggtaa actcaaactc agttttgaag
1021 aaatggaaag gcaaagaaga gaagatgaaa aaaggaaagc agaagaagaa gccagaagga
1081 gaatagagga agaaaagaag gcgtttgctg aagcaaggag aaatatggta gtagatgatg
1141 actccccaga gatgtataag acaatctctc aagaatttct tacaccggga aaactggaaa
1201 ttaattttga agaattatta aaacaaaaaa tggaagaaga aaaacgacga acagaggagg
1261 aacggaagca taagctagaa atggagaaac aagaatttga acaactgaga caggaaatgg
1321 gagaggaaga ggaagaaaat gaaacctttg gattgagcag agaatatgaa gaactgatca
1381 aattaaaaag gagtggctct attcaagcta aaaacctaaa aagcaagttt gaaaaaattg
1441 gacagttgtc tgaaaaagaa atacagaaaa aaatagaaga agagcgagca agaaggagag
1501 caattgacct tgaaattaaa gagcgagaag ctgaaaattt tcatgaggaa gatgatgttg
1561 atgttaggcc tgcaagaaaa agcgaggctc catttactca caaagtgaat atgaaagcta
1621 gatttgaaca aatggctaag gcaagagaag aagaagaaca aagaagaatt gaagaacaaa
1681 agttactacg catgcagttt gaacaaaggg aaattgatgc agcactacaa aagaaaagag
1741 aagaggagga ggaggaagaa ggtagcatca tgaatggctc cactgctgaa gatgaagagc
1801 aaaccagatc aggagctcca tggttcaaga agcctcttaa aaacacatca gttgtagaca
1861 gtgagccagt cagatttacg gttaaagtaa caggagaacc caaaccagaa attacatggt
1921 ggtttgaagg agaaatactg caggatggag aagactatca atatattgaa aggggagaaa
1981 cttactgcct ttacttacca gaaactttcc cagaagatgg aggagagtat atgtgtaaag
2041 cagtcaacaa taaaggatct gcagctagta cctgtattct taccattgaa agtaagaatt
2101 aatcactctt tttatctttt attctattaa ttttttttcc ttaaaatcac ttttcttctt
2161 ctctttttta gctgatgact actagctccc ctcccctctc cctggaac
//