LOCUS AK298547 2466 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ59286 partial cds, highly similar to
Pre-mRNA-processing factor 40 homolog A.
ACCESSION AK298547
VERSION AK298547.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2466)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2466
/cell_type="normal mesangial cells (NHMC56046-2)"
/clone="MESAN2010597"
/clone_lib="MESAN2"
/db_xref="H-InvDB:HIT000493171"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal mesangial cells"
/organism="Homo sapiens"
CDS 19..>2466
/codon_start=1
/note="highly similar to Pre-mRNA-processing factor 40
homolog A"
/protein_id="BAG60744.1"
/transl_table=1
/translation="MCSGSGRRRSSLSPTMRPGTGAERGGLMMGHPGMHYAPMGMHPM
GQRANMPPVPHGMMPQMMPPMGGPPMGQMPGMMSSVMPGMMMSHMSQASMQPALPQLL
SKCPWKEYKSDSGKPYYYNSQTKESRWAKPKELEDLEGYQNTIVAGSLITKSNLHAMI
KAEESSKQEECTTTSTAPVPTTEIPTTMSTMAAAEAAAAVVAAAAAAAAAAAAANANA
STSASNTVSGTVPVVPEPEVTSIVATVVDNENTVTISTEEQAQLTSTPAIQDQSVEVS
SNTGEETSKQETVADFTPKKEEEESQPAKKTYTWNTKEEAKQAFKELLKEKRVPSNAS
WEQAMKMIINDPRYSALAKLSEKKQAFNAYKVQTEKEEKEEARSKYKEAKESFQRFLE
NHEKMTSTTRYKKAEQMFGEMEVWNAISERDRLEIYEDVLFFLSKKEKEQAKQLRKRN
WEALKNILDNMANVTYSTTWSEAQQYLMDNPTFAEDEELQNMDKEDALICFEEHIRAL
EKEEEEEKQKSLLRERRRQRKNRESFQIFLDELHEHGQLHSMSSWMELYPTISSDIRF
TNMLGQPVFSLGSTALDLFKFYVEDLKARYHDEKKIIKDILKDKGFVVEVNTTFEDFV
AIISSTKRSTTLDAGNIKLAFNSLLEKAEAREREREKEEARKMKRKESAFKSMLKQAA
PPIELDAVWEDIRERFVKEPAFEDITLESERKRIFKDFMHVLEHECQHHHSKNKKHSK
KSKKHHRKRSRSRSGSDSDDDDSHSKKKRQRSESRSASEHSSSAESERSYKKSKKHKK
KSKKRRHKSDSPESDAER"
BASE COUNT 876 a 450 c 553 g 587 t
ORIGIN
1 agatcgcttc ttgttggtat gtgtagcggc agtggccgcc ggcggagcag tctgagcccg
61 acgatgaggc cggggacggg agctgagcgt ggaggcctca tgatggggca ccctggcatg
121 cattatgccc caatgggaat gcaccctatg ggtcagagag cgaatatgcc tcctgtacct
181 catggaatga tgccgcagat gatgccccct atgggagggc caccaatggg acaaatgcct
241 ggaatgatgt cgtcagtaat gcctggaatg atgatgtctc atatgtctca ggcttccatg
301 cagcctgcct taccgcaact cttatctaaa tgcccctgga aggaatacaa atcagattct
361 ggaaagcctt actattataa ttctcaaaca aaagaatctc gctgggccaa acctaaagaa
421 cttgaggatc ttgaaggata ccagaatacc attgttgctg gaagtcttat tacaaaatca
481 aacctgcatg caatgatcaa agctgaagaa agcagtaagc aagaagagtg caccacaaca
541 tcaacagctc cagtccctac aacagaaatt ccgaccacaa tgagcaccat ggctgctgcc
601 gaagcagcag ctgctgttgt tgcagcagca gcagcggcag cagcagcagc agctgcagcc
661 aatgctaatg cttccacttc tgcttctaac actgtcagtg gaactgttcc agttgttcct
721 gagcctgaag ttacttccat tgttgctact gttgtagata atgagaatac agtaactatt
781 tcaactgagg aacaagcaca acttactagt acccctgcta ttcaggatca aagtgtggaa
841 gtatccagta atactggaga agaaacatct aagcaagaaa ctgtagctga ttttactccc
901 aaaaaagaag aggaggagag ccaaccagca aagaaaacat acacttggaa tacaaaggaa
961 gaggcaaagc aagcttttaa agaattattg aaagaaaagc gggtaccatc gaatgcttca
1021 tgggagcagg ctatgaaaat gattattaat gatccacgat acagtgcttt ggcaaagtta
1081 agtgaaaaaa agcaagcctt taatgcctat aaagtccaga cagaaaaaga agaaaaagaa
1141 gaagcaagat caaagtacaa agaggctaag gaatcctttc agcgttttct tgaaaatcat
1201 gagaaaatga cttctacaac cagatacaaa aaagcagagc aaatgtttgg agagatggaa
1261 gtttggaatg caatatcaga acgtgatcgt cttgaaatct atgaagatgt tttgttcttt
1321 ctttcaaaaa aagaaaagga acaagcaaag cagttgcgaa agagaaattg ggaagcctta
1381 aaaaacatac ttgacaacat ggctaatgta acatactcta ccacttggtc tgaagcccag
1441 cagtatctga tggataatcc aacttttgca gaagatgagg agttacaaaa tatggacaaa
1501 gaagatgcat taatttgctt tgaagaacac attcgggctt tagaaaagga ggaagaagaa
1561 gaaaaacaga agagtttgct gagagaaagg agacgacagc gaaaaaatag ggaatctttc
1621 cagatatttt tagatgaatt acatgaacat ggacaactgc attctatgtc atcttggatg
1681 gaattgtatc caactattag ttctgatatt agattcacta atatgcttgg tcagcctgtt
1741 ttttcattag gatcaactgc acttgatctt ttcaagtttt atgttgagga tcttaaagca
1801 cgttatcatg acgagaagaa gataataaaa gacattctaa aggataaagg atttgtagtt
1861 gaagtaaaca ctacttttga agattttgtg gcgataatca gttcaactaa aagatcaact
1921 acattagatg ctggaaatat caaattggct ttcaatagtt tactagaaaa ggcagaagcc
1981 cgtgaacgtg aaagagaaaa agaagaggct cggaagatga aacgaaaaga atctgcattt
2041 aagagtatgt taaaacaagc tgctcctccg atagaattgg atgctgtctg ggaagatatc
2101 cgtgagagat ttgtaaaaga gccagcattt gaggacataa ctctagaatc tgaaagaaaa
2161 cgaatattta aagattttat gcatgtgctt gagcatgaat gtcagcatca tcattcaaag
2221 aacaagaaac attctaagaa atctaaaaaa catcatagga aacgttcccg ctctcgatcg
2281 gggtcagatt cagatgatga tgatagccat tcaaagaaaa aaagacagcg atcagagtct
2341 cgttctgctt cagaacattc ttctagtgca gagtctgaga gaagttataa aaagtcaaaa
2401 aagcataaga agaaaagtaa gaagaggaga cataaatctg actctccaga atccgatgct
2461 gagcga
//