LOCUS AK298546 2526 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ55497 complete cds.
ACCESSION AK298546
VERSION AK298546.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2526)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2526
/cell_type="normal mesangial cells (NHMC56046-2)"
/clone="MESAN2010554"
/clone_lib="MESAN2"
/db_xref="H-InvDB:HIT000493170"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal mesangial cells"
/organism="Homo sapiens"
CDS 106..2319
/codon_start=1
/protein_id="BAG60743.1"
/transl_table=1
/translation="MALCSALMTIFTIKMGTAKQAIDQGRSPVIIDNTNIQAWEMKPY
VEVAIGKGYRVEFHEPETWWKFDPEELEKRNKHGVSRKKIAQMLDRYEYQMSISIVMN
SVEPSHKSTQRPPPPQGRQRERVLKKTGHRLSKTKQKRNRKRNKKQNSQNRIMEENSL
EFLSDLTPGDQDPSQSEEEDIEKTRRESEYPFIDGLQNEVGDFVTGYKEKRWKNKDPK
DSFQNVMSIVELDNTPKNYLSKEGDNLFVSLLLRPNEISVTCPILTQNLSCVTTDDCS
GMKVEKHIRNRHTIALDTQDLSAETSCLFMKKREIVDKNLSHEPILCHQHGIRMSDKV
LREEQVYTTKINHWAFFTTNLSDEDLQLGSDRQPYFGSWPAGPHKFICEQRPKKDRAC
KLAGPDSRGQWIQMIFTSVAASEPGNNPEILTDKLLIGNEDFSPPPETMDSFIETNLF
RSCLPQPDIPKNALESTKNKKRRKKRIFNLVPNFDLLGQSRIGVKEREKCDLLTKNHG
LKITLGEEKDRISERNSEEENKQKLMTFDHHPLWFYLDIIKATPLNIDGQRYSHCLSF
NRLRCSASLYKNYIPSFVLHNLSSIWKPSFTNKKLFLTFESQTRVGNKLNDAGFISPE
ILHSHPDTSCSLGVTSDFHFLNERFDRKLKRWEEPKELPAEDSQDLTSTDYRSLELPL
SQGFAFQLVKLFGSPGVPMESLLPDDYVVPLDWKTLKMIYLQWKMSVEKRQKKIG"
BASE COUNT 897 a 436 c 527 g 666 t
ORIGIN
1 atcttgcgta cggagaggtt cgtgtcttcc taaaagaagg ttttattggg aggtaaaggt
61 caatgcgtag gggtagagta agaattctgc ttggtcagaa tcgtgatggc attgtgttca
121 gcactgatga ctattttcac catcaagatg ggtacagcaa aacaagctat cgatcaggga
181 agatctccag ttataataga taacactaat atacaagctt gggaaatgaa gccatatgtg
241 gaagtggcca taggaaaagg atacagagta gagtttcatg aacctgaaac ttggtggaaa
301 tttgatcctg aagaattaga aaagaggaat aaacatggtg tgtctcgaaa gaagattgct
361 cagatgttgg atcgttatga atatcaaatg tccatttcta ttgtaatgaa ttcagtggaa
421 ccatcacaca aaagcacaca aagacctcct cctccacagg ggagacagag agaaagagtt
481 ttgaagaaaa ctgggcatag gctcagcaaa accaaacaga agaggaacag aaaaagaaac
541 aaaaagcaga acagtcagaa tagaatcatg gaggaaaact cattagaatt cttaagtgat
601 cttacaccgg gagatcagga cccatctcag agtgaagagg aagacattga aaagaccaga
661 agagaatcag aatatccctt cattgatggt ctacaaaatg aagtcggaga ttttgtgact
721 ggatataaag aaaaaagatg gaaaaataaa gatcctaaag acagtttcca aaacgttatg
781 tctatagttg aattagacaa cacaccaaag aattacctct ctaaggaagg tgataacttg
841 tttgtaagtt tgttactgag gccaaatgaa atctccgtta cttgtccaat actgactcaa
901 aacctttcct gtgtaacaac tgatgactgc tctggcatga aggtagaaaa gcatattaga
961 aataggcata ccatagcatt agacacccag gacctttctg cggaaacttc atgcttattt
1021 atgaagaaga gagaaatagt agataaaaat ctctcacatg aacccattct gtgccatcaa
1081 catggaatca gaatgtcaga taaagtttta agagaggaac aagtgtatac aactaaaatc
1141 aatcactggg cttttttcac aaccaattta tctgatgaag atttacagct gggctctgac
1201 agacagccct attttggtag ctggcctgca ggacctcata agtttatatg tgaacagaga
1261 ccaaagaaag atagagcatg taagttggct ggtcctgaca gcagggggca atggattcaa
1321 atgatcttca cttcggtggc agcatcagaa ccaggaaaca atccagaaat attgacagac
1381 aaactactga taggaaatga agatttttca cctccacctg aaactatgga ttcattcata
1441 gaaacaaacc tcttcagaag ctgcttacct caaccggata taccaaagaa tgccttagaa
1501 tcaacaaaaa ataagaaaag gaggaagaaa aggattttca atttggtacc aaattttgac
1561 ttattaggac agagtcgtat cggtgtaaaa gaaagggaga aatgtgacct gttaacaaaa
1621 aaccatggac taaaaattac tttgggagaa gaaaaagata gaatttcaga aaggaacagt
1681 gaagaggaga ataaacaaaa acttatgacc tttgatcatc atccattgtg gttttacctt
1741 gatattatca aagctacccc tttaaatatt gatggacagc gttattctca ttgcctgtca
1801 tttaacagac taaggtgctc tgcatcttta tacaaaaatt atattccttc ttttgtgcta
1861 cataatttat ctagtatttg gaagccatct tttacaaaca agaaactgtt tttgactttc
1921 gaatctcaga caagagtagg taataaacta aatgatgcag ggtttatttc tccagaaatt
1981 ttacatagtc atcctgatac ttcgtgctct ttgggagtca cttctgattt tcacttttta
2041 aatgaaaggt ttgatagaaa gctgaaaaga tgggaagaac ctaaggaatt accagctgag
2101 gacagccaag acttaacaag cactgactac cgttcccttg agctaccatt atcacaaggg
2161 tttgcctttc aattagtaaa gctttttgga tctccaggcg ttccaatgga atccttgttg
2221 cctgatgact atgtggttcc ccttgactgg aagacactaa agatgatcta cttgcaatgg
2281 aagatgtcag tggagaaaag acagaagaag attggttgaa aaatgaaaat tccttgaact
2341 ttgagttctg ctgtcttcat ggtactgctg aagatcatga tcacggagaa aagtcagagt
2401 gctcagtgcc aacccaaggg attctttcca gagacgtacc cgttggatac caaaattagt
2461 ttggataatc tgttcaacca ttcttgataa gttatctgaa taataaaaaa actcaacaga
2521 ggaggt
//