LOCUS AK298542 1964 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58776 complete cds, highly similar to
Nidogen-2 precursor.
ACCESSION AK298542
VERSION AK298542.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1964)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1964
/cell_type="normal mesangial cells (NHMC56046-2)"
/clone="MESAN2009827"
/clone_lib="MESAN2"
/db_xref="H-InvDB:HIT000493166"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal mesangial cells"
/organism="Homo sapiens"
CDS 254..1933
/codon_start=1
/note="highly similar to Nidogen-2 precursor"
/protein_id="BAG60740.1"
/transl_table=1
/translation="MCDTTARCHPGTGVDYTCECASGYQGDGRNCVDENECATGFHRC
GPNSVCINLPGSYRCECRSGYEFADDRHTCIYVDECSENRCHPAATCYNTPGSFSCRC
QPGYYGDGFQCIPDSTSSLTPCEQQQRHAQAQYAYPGARFHIPQCDEQGNFLPLQCHG
STGFCWCVDPDGHEVPGTQTPPGSTPPHCGPSPEPTQRPPTICERWRENLLEHYGGTP
RDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGTRSQPGTTPACIPTVAPPM
VRPTPRPDVTPPSVGTFLLYTQGQQIGYLPLNGTRLQKDAAKTLLSLHGSIIVGIDYD
CRERMVYWTDVAGRTISRAGLELGAEPETIVNSGLISPEGLAIDHIRRTMYWTDSVLD
KIESALLDGSERKVLFYTDLVNPRAIAVDPIRGNLYWTDWNREAPKIETSSLDGENRR
ILINTDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFSIVSY
ADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK"
BASE COUNT 488 a 556 c 497 g 423 t
ORIGIN
1 ctactctctg acttttggtg caatcaacca aacatggtcc taccgcatcc accagaacat
61 cacttaccag gtgtgcaggc acgcccccag acacccgtcc ttccccacca cccagcagct
121 gaacgtggac cgggtctttg ccttgtataa tgacgaagaa agagtgctta gatttgctgt
181 gaccaatcaa attggcccgg tcaaagaaga ttcagacccc actccggtga atccttgcta
241 tgatgggagc cacatgtgtg acacaacagc acggtgccat ccagggacag gtgtagatta
301 cacctgtgag tgcgcatctg ggtaccaggg agatggacgg aactgtgtgg atgaaaatga
361 atgtgcaact ggctttcatc gctgtggccc caactctgta tgtatcaact tgcctggaag
421 ctacaggtgt gagtgccgga gtggttatga gtttgcagat gaccggcata cttgcatcta
481 tgtagatgaa tgctcagaaa acagatgtca ccctgcagct acctgctaca atactcctgg
541 ttccttctcc tgccgttgtc aacccggata ttatggggat ggatttcagt gcatacctga
601 ctccacctca agcctgacac cctgtgaaca acagcagcgc catgcccagg cccagtatgc
661 ctaccctggg gcccggttcc acatccccca atgcgacgag cagggcaact tcctgcccct
721 acagtgtcat ggcagcactg gtttctgctg gtgcgtggac cctgatggtc atgaagttcc
781 tggtacccag actccacctg gctccacccc gcctcactgt ggaccatcac cagagcccac
841 ccagaggccc ccgaccatct gtgagcgctg gagggaaaac ctgctggagc actacggtgg
901 caccccccgg gatgaccagt acgtgcccca gtgcgatgac ctgggccact tcatccccct
961 gcagtgccac ggaaagagcg acttctgctg gtgtgtggac aaagatggca gagaggtgca
1021 gggcacccgc tcccagccag gcaccacccc tgcgtgtata cccaccgtcg ctccacccat
1081 ggtccggccc acgccccggc cagatgtgac ccctccatct gtgggcacct tcctgctcta
1141 tactcagggc cagcagattg gctacttacc cctcaatggc accaggcttc agaaggatgc
1201 agctaagacc ctgctgtctc tgcatggctc cataatcgtg ggaattgatt acgactgccg
1261 ggagaggatg gtgtactgga cagatgttgc tggacggaca atcagccgtg ctggtctgga
1321 actgggagca gagcctgaga cgatcgtgaa ttcaggtctg ataagccctg aaggacttgc
1381 catagaccac atccgcagaa caatgtactg gacggacagt gtcctggata agatagagag
1441 cgccctgctg gatggctctg agcgcaaggt cctcttctac acagatctgg tgaatccccg
1501 tgccatcgct gtggatccaa tccgaggcaa cttgtactgg acagactgga atagagaagc
1561 tcctaaaatt gaaacgtcat ctttagatgg agaaaacaga agaattctga tcaatacaga
1621 cattggattg cccaatggct taacctttga ccctttctct aaactgctct gctgggcaga
1681 tgcaggaacc aaaaaactgg agtgtacact acctgatgga actggacggc gtgtcattca
1741 aaacaacctc aagtacccct tcagcatcgt aagctatgca gatcacttct accacacaga
1801 ctggaggagg gatggtgttg tatcagtaaa taaacatagt ggccagttta ctgatgagta
1861 tctcccagaa caacgatctc acctctacgg gataactgca gtctacccct actgcccaac
1921 aggaagaaag taagtacagt aatgtaaagg aagacttgga gttt
//