LOCUS AK298541 2466 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ56123 complete cds, highly similar to
Calpastatin.
ACCESSION AK298541
VERSION AK298541.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2466)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2466
/cell_type="normal mesangial cells (NHMC56046-2)"
/clone="MESAN2009545"
/clone_lib="MESAN2"
/db_xref="H-InvDB:HIT000493165"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal mesangial cells"
/organism="Homo sapiens"
CDS 212..2296
/codon_start=1
/note="highly similar to Calpastatin"
/protein_id="BAH12810.1"
/transl_table=1
/translation="MEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPK
EHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAG
ITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEEN
TTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAI
DALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQA
LEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKD
GKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEA
VCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKE
EDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGP
QNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQ
RQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKS
EDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSA
KTTEETSKPKDD"
BASE COUNT 868 a 541 c 584 g 473 t
ORIGIN
1 agagagctgg gctggagctc caagcggaaa ccgcgggagc cgagcccagc taggaatgca
61 gacctcctga aaaccaagcc gaggactgcg gggtccggtg tccacgcaga gtgtcagctt
121 cctctggtgc aaccagcaag tcttccagta tgaatcccac agaaaccaag gtatgaagaa
181 tgctaattga gccattccag tcagccaaca gatggaagga ccacatcttc ctaacaagaa
241 aaaacacaaa aaacaggctg taaaaacaga acctgagaag aagtcacagt caaccaagct
301 gtctgtggtt catgagaaaa aatcccaaga aggaaagcca aaagaacaca cagagccaaa
361 aagcctaccc aagcaggcat cagatacagg aagtaacgat gctcacaata aaaaagcagt
421 ttccagatca gctgaacagc agccatcaga gaaatcaaca gaaccaaaga ctaaaccaca
481 agacatgatt tctgctggtg gagagagtgt tgctggtatc actgcaatat ctggcaagcc
541 gggtgacaag aaaaaagaaa agaaatcatt aaccccagct gtgccagttg aatctaaacc
601 ggataaacca tcgggaaagt caggcatgga tgctgctttg gatgacttaa tagatacttt
661 aggaggacct gaagaaactg aagaagaaaa tacaacgtat actggaccag aagtttcaga
721 tccaatgagt tccacctaca tagaggaatt gggtaaaaga gaagtcacaa ttcctccaaa
781 atatagggaa ctattggcta aaaaggaagg gatcacaggg cctcctgcag actcttcgaa
841 acccataggg ccagatgatg ctatagacgc cttgtcatct gacttcacct gtgggtcgcc
901 tacagctgct ggaaagaaaa ctgaaaaaga ggaatctaca gaagttttaa aagctcagtc
961 agcagggaca gtcagaagtg ctgctccacc ccaagagaag aaaagaaagg tggagaagga
1021 tacaatgagt gatcaagcac tcgaggctct gtcggcttca ctgggcaccc ggcaagcaga
1081 acctgagctc gacctccgct caattaagga agtcgatgag gcaaaagcta aagaagaaaa
1141 actagagaag tgtggtgagg atgatgaaac aatcccatct gagtacagat taaaaccagc
1201 cacggataaa gatggaaaac cactattgcc agagcctgaa gaaaaaccca agcctcggag
1261 tgaatcagaa ctcattgatg aactttcaga agattttgac cggtctgaat gtaaagagaa
1321 accatctaag ccaactgaaa agacagaaga atctaaggcc gctgctccag ctcctgtgtc
1381 ggaggctgtg tgtcggacct ccatgtgtag tatacagtca gcaccccctg agccggctac
1441 cttgaagggc acagtgccag atgatgctgt agaagccttg gctgatagcc tggggaaaaa
1501 ggaagcagat ccagaagatg gaaaacctgt gatggataaa gtcaaggaga aggccaaaga
1561 agaagaccgt gaaaagcttg gtgaaaaaga agaaacaatt cctcctgatt atagattaga
1621 agaggtcaag gataaagatg gaaagccact cctgccaaaa gagtctaagg aacagctccc
1681 acccatgagt gaagacttcc ttctggatgc tttgtctgag gacttctctg gtccacaaaa
1741 tgcttcatct cttaaatttg aagatgctaa acttgctgct gccatctctg aagtggtttc
1801 ccaaacccca gcttcaacga cccaagctgg agccccaccc cgtgatacct cgcagagtga
1861 caaagacctc gatgatgcct tggataaact ctctgacagt ctaggacaaa ggcagcctga
1921 cccagatgag aacaaaccaa tggaagataa agtaaaggaa aaagctaaag ctgaacatag
1981 agacaagctt ggagaaagag atgacactat cccacctgaa tacagacatc tcctggatga
2041 taatggacag gacaaaccag tgaagccacc tacaaagaaa tcagaggatt caaagaaacc
2101 tgcagatgac caagacccca ttgatgctct ctcaggagat ctggacagct gtccctccac
2161 tacagaaacc tcacagaaca cagcaaagga taagtgcaag aaggctgctt ccagctccaa
2221 agcacctaag aatggaggta aagcgaagga ttcagcaaag acaacagagg aaacttccaa
2281 gccaaaagat gactaaagaa atacaagtta aggtatctgg tatctgcatg taaaatcttc
2341 agctggtgga tggtgacttt tgaagaacaa aaggctttgg caacagaaaa caattgttct
2401 gggtgatttc tagaatggtt tttgttgagt ctctgaacat cctaaatatt ggtttgttat
2461 tctttt
//