LOCUS AK298495 1142 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ60776 complete cds.
ACCESSION AK298495
VERSION AK298495.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1142)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1142
/cell_type="normal mesangial cells (NHMC56046-2)"
/clone="MESAN2000155"
/clone_lib="MESAN2"
/db_xref="H-InvDB:HIT000493119"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal mesangial cells"
/organism="Homo sapiens"
CDS 71..616
/codon_start=1
/protein_id="BAG60703.1"
/transl_table=1
/translation="MSGSVLFTAGERWRCFLTPSRSSLYWALHNFCCRKKSTTPKKIT
PNVTFCDENAKEPENALDKLFSSEQQASILHVLNTASTKELEAFRLLRGRRSINIVEH
RENFGPFQNLESLMNVPLFKYKSTVQVCNSILCPKTGREKRKSPENRFLRKLLKPDIE
RERLKVYSSCLLYCLIFENLL"
BASE COUNT 354 a 223 c 244 g 321 t
ORIGIN
1 gcttttgggc gcagcacgca ggaaccggaa gtcaatggag gaagactgga cctactgatt
61 caacttggag atgagcgggt ctgtcctctt cacggcggga gagaggtgga gatgctttct
121 gaccccgtcg aggtcatccc tgtactgggc cttacataat ttctgctgtc ggaaaaaatc
181 cactacacct aagaaaatta ctcccaatgt tactttttgt gatgaaaatg caaaggagcc
241 cgaaaatgca cttgacaagc tcttctcttc agaacagcag gcttccatct tgcatgtgtt
301 gaatacagca tctactaaag aacttgaagc tttccgattg cttcgtggaa gaaggtccat
361 caatatcgta gagcacagag aaaactttgg gccatttcag aatttagaga gtttaatgaa
421 tgtgcccttg tttaagtata aaagtacagt tcaagtttgt aactccatac tttgtccaaa
481 gactggacgg gaaaaaagaa agtcaccgga aaaccggttc ctgagaaagc tcctcaaacc
541 agacatagaa agagaaagac ttaaggtata ttcttcatgt ctgctatact gcctaatatt
601 tgagaaccta ctctagaaag cactcaagta ttaaacctta gaggaactca tgtcctgtgt
661 aacattgctt gataatcatt atctcataaa aatcataagc accatttttt ttctggacca
721 gtgggtccgt tttttgagga atatgggtac ttttgaaaac ctgatgaaag ctgtggaccc
781 tcttcccata gaaaaagata tagcacatat acacagaata ctacacagtt tcagaggact
841 ggcagattct gttaagtcca ctcatggact ccaagtagaa ctgctgcaat tgatctggaa
901 catattttaa ccaaattctt cagggaggat tctcatccag attcatggcc aaaactattt
961 atacccagat tagagtggag ttgcatctta tttagggggc tggattttaa gtcagttttt
1021 atggagatat aagtcatgca gtaaaatcct ttaagaaaaa atcgattgcc gacgaagtat
1081 tgtgtgtagt ctggttcagt aatgcttgtg cacagtaaag cttgagagtc acttagctaa
1141 ag
//