LOCUS AK298195 982 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ57492 complete cds, highly similar to
Neuronal acetylcholine receptor protein subunit alpha-3 precursor.
ACCESSION AK298195
VERSION AK298195.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 982)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..982
/cell_line="IMR32"
/cell_type="neuroblastoma"
/clone="IMR322010855"
/clone_lib="IMR322"
/db_xref="H-InvDB:HIT000492819"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 70..576
/codon_start=1
/note="highly similar to Neuronal acetylcholine receptor
protein subunit alpha-3 precursor"
/protein_id="BAG60465.1"
/transl_table=1
/translation="MPSWVKTVFLNLLPRVMFMTRPTSNEGNAQKPRPLYGAELSNLN
CFSRAESKGCKEGYPCQDGMCGYCHHRRIKISNFSANLTRSSSSESVDAVLSLSALSP
EIKEAIQSVKYIAENMKAQNEAKEIQDDWKYVAMVIDRIFLWVFTLVCILGTAGLFLQ
PLMAREDA"
BASE COUNT 274 a 217 c 214 g 277 t
ORIGIN
1 gtaaccttgt ccatcgtcat caccgtcttc gtgctcaacg tgcactacag aaccccgacg
61 acacacacaa tgccctcatg ggtgaagact gtattcttga acctgctccc cagggtcatg
121 ttcatgacca ggccaacaag caacgagggc aacgctcaga agccgaggcc cctctacggt
181 gccgagctct caaatctgaa ttgcttcagc cgcgcagagt ccaaaggctg caaggagggc
241 tacccctgcc aggacgggat gtgtggttac tgccaccacc gcaggataaa aatctccaat
301 ttcagtgcta acctcacgag aagctctagt tctgaatctg ttgatgctgt gctgtccctc
361 tctgctttgt caccagaaat caaagaagcc atccaaagtg tcaagtatat tgctgaaaat
421 atgaaagcac aaaatgaagc caaagagatt caagatgatt ggaagtatgt tgccatggtg
481 attgatcgta tttttctgtg ggttttcacc ctggtgtgca ttctagggac agcaggattg
541 tttctgcaac ccctgatggc cagggaagat gcataagcac taagctgtgt gcctgcctgg
601 gagacttcct tgtgccaggg caggaggagg ctgcttccta gtaagaacgt actttctgtt
661 atcaagctac cagctttgtt tttggcattt cgaggtttac ttattttcca cttatcttgg
721 aatcatgcaa aaaaaaaatg tcaagagtat ttattaccga taaatgaaca tttaactagc
781 ctttttggta tggtaaagag atgtcaaaat gtgattctac gtgattagta tgctatgcta
841 tggaatatac atgtaaaaat gtttcctttt agttgttgaa acaaaactgg atagaaaaat
901 gctgttcaga aatatgaaaa gtcattcagt tatcactaca gatctcccag taatttttct
961 tatttagccc ataatctctt tg
//