LOCUS AK298191 2263 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ61327 complete cds, highly similar to Exocyst
complex component 4.
ACCESSION AK298191
VERSION AK298191.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2263)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2263
/cell_line="IMR32"
/cell_type="neuroblastoma"
/clone="IMR322009024"
/clone_lib="IMR322"
/db_xref="H-InvDB:HIT000492815"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 161..1942
/codon_start=1
/note="highly similar to Exocyst complex component 4"
/protein_id="BAH12738.1"
/transl_table=1
/translation="MADVWVKIQDVLQMLLTEYLDMKNTRTASEPSAQLSYASTGREF
AAFFAKKKPQRPKNSLFKFESSSHAISMSAYLREQRRELYSRSGELQGGPDDNLIEGG
GTKFVCKPGARNITVIFHPLLRFIQEIEHALGLGPAKQCPLREFLTVYIKNIFLNQVL
AEINKEIEGVTKTSDPLKILANADTMKVLGVQRPLLQSTIIVEKTVQDLLNLMHDLSA
HSDQFLNMVCVKLQEYKDTCTAAYRGIVQSEEKLVISASWAKDDDISRLLKSLPNWMN
MAQPKQLRPKREEEEDFIRAAFGKESEVLIGNLGDKLIPPQDILRDVSDLKALANLHE
SLEWLASRTKSAFSNLSTSQMLSPAQDSHTNTDLPPVSEQIMQTLSELAKSFQDMADR
CLLVLHLEVRVHCFHYLIPLAKEGNYAIVANVESMDYDPLVVKLNKDISAIEEAMSAS
LQQHKFQYIFEGLGHLISCILINGAQYFRRISESGIKKMCRNIFVLQQNLTNITMSRE
ADLDFARQYYEMLYNTADELLNLVVDQGVKYTELEYIHALTLLHRSQTGVGELTTQNT
RLQRLKEIICEQAAIKQATKDKKITTV"
BASE COUNT 631 a 523 c 554 g 555 t
ORIGIN
1 ctagcggctc tccccgcgtc caagatggcg gcagaagcag ctggagttac tgtttgacaa
61 gtttaatgct gtagccgctg cacactctgt ggtcctggga tacctgcagg acactgtagt
121 gactccactg actcagcagg aagatatcaa actgtatgat atggcagatg tatgggtgaa
181 gatccaagat gttctacaga tgctattaac tgagtacttg gatatgaaga atactcgtac
241 ggcctctgaa ccatcagctc aactaagcta tgccagcact ggacgagagt ttgcagcctt
301 ttttgccaag aagaaacctc aaaggccaaa aaattctctt ttcaagttcg aatcgtcctc
361 ccatgccatc agtatgagcg cctatctgcg agaacagaga agggagctct atagtcgaag
421 tggagaactg caagggggtc ctgatgacaa cttaattgaa ggtggaggaa caaaatttgt
481 ctgcaaacct ggagccagaa acattaccgt catattccac ccattactaa gatttattca
541 ggagattgag catgctctgg gtcttggccc agccaaacag tgtcctcttc gagagtttct
601 caccgtgtac atcaaaaaca tctttctcaa tcaagtcttg gctgagatca acaaggagat
661 tgaaggagtc actaaaacat ctgacccttt gaagattctg gccaacgcag acaccatgaa
721 ggtgctggga gtgcagcggc ctctcctaca gagcacaatc attgtggaga agacagttca
781 agacctcctg aacctgatgc atgacttgag tgcacattca gatcaattcc tcaacatggt
841 gtgcgtaaag ctccaggagt acaaggacac ctgcactgca gcttacaggg gtattgtcca
901 gtcagaagaa aaacttgtca tcagtgcatc ctgggcaaaa gatgatgata tcagcagact
961 cttgaaatct ctaccaaact ggatgaatat ggctcaaccc aaacagctga ggccaaaaag
1021 agaggaggaa gaagatttca taagggcagc ttttggcaag gagtctgaag ttcttattgg
1081 gaacctgggt gataaattaa tccctccaca agacatcctt cgtgacgtca gtgacctcaa
1141 agccttggcc aacctgcatg aaagcctgga atggttggca agtcgaacaa agtcagcttt
1201 ctccaatctt tctacatccc agatgctttc tcctgctcaa gacagccaca cgaacacgga
1261 tctcccccca gtgtcagagc agatcatgca gactctcagt gaacttgcca aatcgttcca
1321 ggatatggct gaccgctgct tgcttgtctt acatctggaa gtgagggttc actgtttcca
1381 ctatcttatc cctcttgcaa aggaggggaa ctatgccatt gtggctaatg tggaaagtat
1441 ggattatgac cccctggtgg tcaagctcaa caaagatatc agcgccattg aagaggccat
1501 gagcgccagc cttcagcagc acaagttcca gtatatcttc gaaggcctgg gccacctgat
1561 ctcctgcatc ctcattaatg gtgcccagta cttcaggcgc atcagtgagt ctggcatcaa
1621 gaaaatgtgt aggaacattt ttgttcttca gcagaatttg accaacatca ccatgtcgcg
1681 ggaggcagac ctggactttg caaggcagta ctacgagatg ctttacaaca cagctgacga
1741 gctcctgaac ctggtggtgg accagggtgt gaagtacacg gagctggagt acatccacgc
1801 tctgaccctg ctgcaccgca gccagactgg ggtgggggaa ctgaccaccc agaacacgag
1861 gctgcagagg ctcaaagaga tcatctgcga gcaggctgcc atcaagcaag ccaccaagga
1921 caagaagata actaccgttt agcagggcgt actgcggttg gtgacggggg tcccctcagt
1981 cacactcact tttttccttg gtatgttatt gagtatattc tgagcttagt tttctctaca
2041 gtgatacttt agtggagagg aggtgtaagg attctttctc tctggttttg gcttttcata
2101 taaatgctaa aggaaggcga cagtacagag tgttttggtt gaacaactac tttaatgcag
2161 tcaaatctaa cgggactagg gtgggatagg gaggaaggtg gtatcaaatt gttggactct
2221 gaaaaacaag tggatggatt actaatattt gatttattac tca
//