LOCUS AK298139 1510 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ54532 complete cds, highly similar to Mothers
against decapentaplegic homolog 3 (SMAD3).
ACCESSION AK298139
VERSION AK298139.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1510)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1510
/cell_type="synoviocytes from rheumatioid arthritis
(HS-RA)"
/clone="HSYRA2007134"
/clone_lib="HSYRA2"
/db_xref="H-InvDB:HIT000492763"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, synoviocytes from rheumatioid
arthritis"
/organism="Homo sapiens"
CDS 7..1152
/codon_start=1
/note="highly similar to Mothers against decapentaplegic
homolog 3 (SMAD3)"
/protein_id="BAH12731.1"
/transl_table=1
/translation="MSCLHPRQTWKGAALVHRKAWWMGRSLDGRLQVSHRKGLPHVIY
CRLWRWPDLHSHHELRAMELCEFAFNMKKDEVCVNPYHYQRVETPVLPPVLVPRHTEI
PAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGS
PNLSPNPMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRVGETFHASQPSMTVDGFTD
PSNSERFCLGLLSNVNRNAAVELTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNC
NQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVK
GWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSIRCSSVS"
BASE COUNT 360 a 451 c 396 g 303 t
ORIGIN
1 acaaacatgt cttgcctgca ccctaggcaa acgtggaaag gcgcagctct ggtacaccgg
61 aaagcatggt ggatggggag gtccctggat ggccggttgc aggtgtccca tcggaagggg
121 ctccctcatg tcatctactg ccgcctgtgg cgatggccag acctgcacag ccaccacgag
181 ctgcgggcca tggagctgtg tgagttcgcc ttcaatatga agaaggacga ggtctgcgtg
241 aatccctacc actaccagag agtagagaca ccagttctac ctcctgtgtt ggtgccacgc
301 cacacagaga tcccggccga gttcccccca ctggacgact acagccattc catccccgaa
361 aacactaact tccccgcagg catcgagccc cagagcaata ttccagagac cccaccccct
421 ggctacctga gtgaagatgg agaaaccagt gaccaccaga tgaaccacag catggacgca
481 ggttctccaa acctatcccc gaatccgatg tccccagcac ataataactt ggacctgcag
541 ccagttacct actgcgagcc ggccttctgg tgctccatct cctactacga gctgaaccag
601 cgcgtcgggg agacattcca cgcctcgcag ccatccatga ctgtggatgg cttcaccgac
661 ccctccaatt cggagcgctt ctgcctaggg ctgctctcca atgtcaacag gaatgcagca
721 gtggagctga cacggagaca catcggaaga ggcgtgcggc tctactacat cggaggggag
781 gtcttcgcag agtgcctcag tgacagcgct atttttgtcc agtctcccaa ctgtaaccag
841 cgctatggct ggcacccggc caccgtctgc aagatcccac caggatgcaa cctgaagatc
901 ttcaacaacc aggagttcgc tgccctcctg gcccagtcgg tcaaccaggg ctttgaggct
961 gtctaccagt tgacccgaat gtgcaccatc cgcatgagct tcgtcaaagg ctggggagcg
1021 gagtacagga gacagactgt gaccagtacc ccctgctgga ttgagctgca cctgaatggg
1081 cctttgcagt ggcttgacaa ggtcctcacc cagatgggct ccccaagcat ccgctgttcc
1141 agtgtgtctt agagacatca agtatggtag gggagggcag gcttggggaa aatggccatg
1201 caggaggtgg agaaaattgg aactctactc aacccattgt tgtcaaggaa gaagaaatct
1261 ttctccctca actgaagggg tgcacccacc tgttttctga aacacacgag caaacccaga
1321 ggtggatgtt atgaacagct gtgtctgcca aacacattta ccctttggcc ccactttgaa
1381 gggcaagaaa tggcgtctgc tctggtggct taagtgagca gaacaggtag tattacacca
1441 ccggccccct ccccccagac tctttttttg agtgacagct ttctgggatg tcacagtcca
1501 accagaaaca
//