LOCUS AK298039 1972 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ54278 complete cds, highly similar to
SPARC-like protein 1 precursor.
ACCESSION AK298039
VERSION AK298039.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1972)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1972
/clone="HLUNG2006977"
/clone_lib="HLUNG2"
/db_xref="H-InvDB:HIT000492663"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="lung"
CDS 179..1738
/codon_start=1
/note="highly similar to SPARC-like protein 1 precursor"
/protein_id="BAG60338.1"
/transl_table=1
/translation="MKTGLFFLCLLGTAAAIPTNARLLSDHSKPTAETVAPDNTAIPS
LRAEDEENEKETAVSTEDDSHHKAEKSSVLKSKEESHEQSAEQGKSSSQELGLKDQED
SDGDLSVNLEYAPTEGTLDIKEDNSNAEMEEENASNVNKHIQETEWQSQEGKTGLEAI
SNHKETEEKTVSEALLMEPTDDGNTTPRNHGVDDDGDDDGDDGGTDGPRHSASDDYFI
PSQAFLEAERAQSIAYHLKIEEQREKVHENENIGTTEPGEHQEAKKAENSSNEEETSS
EGNMRVHAVDSCMSFQCKRGHICKADQQGKPHCVCQDPVTCPPTKPLDQVCGTDNQTY
ASSCHLFATKCRLEGTKKGHQLQLDYFGACKSIPTCTDFEVIQFPLRMRDWLKNILMQ
LYEANSEHAGYLNEKQRNKVKKIYLDEKRLLAGDHPIDLLLRDFKKNYHMYVYPVHWQ
FSELDQHPMDRVLTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGI
KEEDIDENLLF"
BASE COUNT 648 a 406 c 451 g 467 t
ORIGIN
1 ccagtagact tgaatgtgcc tctgggcaaa gaagcagagc taacgaggaa agggatttaa
61 agagtttttc ttgggtgttt gtcaaacttt tattccctgt ctgtgtgcag aggggattca
121 acttcaattt ttctgcagtg gctctgagtc cagcccctta cttaaagatc tggaaagcat
181 gaagactggg ctttttttcc tatgtctctt gggaactgca gctgcaatcc cgacaaatgc
241 aagattatta tctgatcatt ccaaaccaac tgctgaaacg gtagcacccg acaacactgc
301 aatccccagt ttaagggctg aagatgaaga aaatgaaaaa gaaacagcag tatccacaga
361 agacgattcc caccataagg ctgaaaaatc atcagtacta aagtcaaaag aggaaagcca
421 tgaacagtca gcagaacagg gcaagagttc tagccaagag ctgggattga aggatcaaga
481 ggacagtgat ggtgacttaa gtgtgaattt ggagtatgca ccaactgaag gtacattgga
541 cataaaagaa gataactcca atgcagaaat ggaagaggaa aatgcatcga acgtcaataa
601 gcacattcaa gaaactgaat ggcagagtca agagggtaaa actggcctag aagctatcag
661 caaccacaaa gagacagaag aaaagactgt ttctgaggct ctgctcatgg aacctactga
721 tgatggtaat accacgccca gaaatcatgg agttgatgat gacggcgatg atgatggcga
781 tgatggcggc actgatggcc ccaggcacag tgcaagtgat gactacttca tcccaagcca
841 ggcctttctg gaggccgaga gagctcaatc cattgcctat cacctcaaaa ttgaggagca
901 aagagaaaaa gtacatgaaa atgaaaatat aggtaccact gagcctggag agcaccaaga
961 ggccaagaaa gcagagaact catcaaatga ggaggaaacg tcaagtgaag gcaacatgag
1021 ggtgcatgct gtggattctt gcatgagctt ccagtgtaaa agaggccaca tctgtaaggc
1081 agaccaacag ggaaaacctc actgtgtctg ccaggatcca gtgacttgtc ctccaacaaa
1141 accccttgat caagtttgtg gcactgacaa tcagacctat gctagttcct gtcatctatt
1201 cgctactaaa tgcagactgg aggggaccaa aaaggggcat caactccagc tggattattt
1261 tggagcctgc aaatctattc ctacttgtac ggactttgaa gtgattcagt ttcctctacg
1321 gatgagagac tggctcaaga atatcctcat gcagctttat gaagccaact ctgaacacgc
1381 tggttatcta aatgagaagc agagaaataa agtcaagaaa atttacctgg atgaaaagag
1441 gcttttggct ggggaccatc ccattgacct tctcttaagg gactttaaga aaaactacca
1501 catgtatgtg tatcctgtgc actggcagtt tagtgaactt gaccaacacc ctatggatag
1561 agtcttgaca cattctgaac ttgctcctct gcgagcatct ctggtgccca tggaacactg
1621 cataacccgt ttctttgagg agtgtgaccc caacaaggat aagcacatca ccctgaagga
1681 gtggggccac tgctttggaa ttaaagaaga ggacatagat gaaaatctct tgttttgaac
1741 gaagatttta aagaactcaa ctttccagca tcctcctctg ttctaaccac ttcagaaata
1801 tatgcagctg tgatacttgt agatttatat ttagcaaaat gttagcatgt atgacaagac
1861 aatgagagta attgcttgac aacaacctat gcaccaggta tttaacatta actttggaaa
1921 caaaaatgta caattaagta aagtcaacat atgcaaaata ctgtacattg tg
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