LOCUS AK297939 2011 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ57129 complete cds, highly similar to
1,4-alpha-glucan branching enzyme (EC 2.4.1.18).
ACCESSION AK297939
VERSION AK297939.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2011)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2011
/cell_type="dermal papilla cells (HDPC)"
/clone="HHDPC2002354"
/clone_lib="HHDPC2"
/db_xref="H-InvDB:HIT000492563"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, dermal papilla cells"
/organism="Homo sapiens"
CDS 428..1825
/codon_start=1
/note="highly similar to 1,4-alpha-glucan branching
enzyme (EC 2.4.1.18)"
/protein_id="BAG60255.1"
/transl_table=1
/translation="MAIMEHAYYASFGYQITSFFAASSRYGTPEELQELVDTAHSMGI
IVLLDVVHSHASKNSADGLNMFDGTDSCYFHSGPRGTHDLWDSRLFAYSSWEVLRFLL
SNIRWWLEEYRFDGFRFDGVTSMLYHHHGVGQGFSGDYSEYFGLQVDEDALTYLMLAN
HLVHTLCPDSITIAEDVSGMPALCSPISQGGGGFDYRLAMAIPDKWIQLLKEFKDEDW
NMGDIVYTLTNRRYLEKCIAYAESHDQALVGDKSLAFWLMDAEMYTNMSVLTPFTPVI
DRGIQLHKMIRLITHGLGGEGYLNFMGNEFGHPEWLDFPRKGNNESYHYARRQFHLTD
DDLLRYKFLNNFDRDMNRLEERYGWLAAPQAYVSEKHEGNKIIAFERAGLLFIFNFHP
SKSYTDYRVGTALPGKFKIVLDSDAAEYGGHQRLDHSTDFFSEAFEHNGRPYSLLVYI
PSRVALILQNVDLPN"
BASE COUNT 532 a 441 c 476 g 562 t
ORIGIN
1 ctcggcttgc ctcggcttgc ctcgacacgc ctaggcgccc tccggctccg ccctagccgc
61 cgcgtcccag ctagagctcc agcacccgct caggccccac tcgaccctct cgggcctcgg
121 ctacttggac tgcggcggaa tatggcggct ccgatgactc ccgcggctcg gcccgaggac
181 tacgaggcgg cgctcaatgc cgccctggct gacgtgcccg aactggccag actcctggag
241 atcgacccgt acttgaagcc ctacgccgtg gacttccagc gcaggtataa gcagtttagc
301 caaattttga agaacattgg ataatgtggg aatttcttcc catgaaggaa aagtagcttc
361 ttataaacat tttacatgca atgtactacc aagaatcaaa ggccttggat acaactgcat
421 tcagttgatg gcaatcatgg agcatgctta ctatgccagc tttggttacc aaatcacaag
481 cttctttgca gcttccagcc gttatggaac acctgaagag ctacaagaac tggtagacac
541 agctcattcc atgggtatca tagtcctctt agatgtggta cacagccatg cttcaaaaaa
601 ttcagcagat ggattgaata tgtttgatgg gacagattcc tgttattttc attctggacc
661 tagagggact catgatcttt gggatagcag attgtttgcc tactccagct gggaagtttt
721 aagattcctt ctgtcaaaca taagatggtg gttggaagaa tatcgctttg atggatttcg
781 ttttgatggt gttacgtcca tgctttatca tcaccatgga gtgggtcaag gtttctcagg
841 tgattacagt gaatatttcg gactacaagt agatgaagat gccttgactt acctcatgtt
901 ggcaaatcat ttggttcaca cgctgtgtcc cgattctata acaatagctg aggatgtatc
961 aggaatgcca gctctgtgct ctccaatttc ccagggaggg ggtggttttg actatcgact
1021 agccatggca attccagata agtggattca gctacttaaa gagtttaaag atgaagactg
1081 gaacatgggc gatatagtat acacgctcac aaacaggcgc taccttgaaa agtgcattgc
1141 ttatgcagag agccatgatc aggcattggt tggggataag tcgctggcat tttggttgat
1201 ggatgccgaa atgtatacaa acatgagtgt cctgactcct tttactccag ttattgatcg
1261 tggaatacag cttcataaaa tgattcgact cattacgcat gggcttggtg gagaaggcta
1321 tctcaatttc atgggtaatg aatttgggca tcctgaatgg ttagacttcc caagaaaagg
1381 aaataatgag agttaccatt atgccaggcg gcagtttcat ttaactgacg acgaccttct
1441 tcgctacaag ttcctaaata attttgacag ggatatgaat agattggaag aaagatatgg
1501 ttggcttgca gctccacagg cctacgtgag tgaaaaacat gaaggcaata agatcattgc
1561 ttttgaaaga gcaggtcttc ttttcatttt caacttccat ccaagcaaga gctacactga
1621 ctaccgagtt ggaacagcat tgccagggaa attcaaaatt gtgctagatt cagatgcagc
1681 ggaatatgga gggcatcaga gactggacca cagcactgac tttttttctg aggcttttga
1741 acataatggg cgtccctatt ctcttttggt gtacattcca agcagagtgg ccctcatcct
1801 tcagaatgtg gatctgccga attgaagagg cctgatttca gctccaccag atgcagattt
1861 gtgttttgtt ttcttgttat cactgtcaca cagcttataa catgtatgct tttcagaata
1921 cagttgtcta gccaagccat caagtgtctg aaattcaata ttggtttatg caaatacagc
1981 aaacttttat ttaagtagat gggagaatat g
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