LOCUS AK297879 2339 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ59277 complete cds, highly similar to
Calpastatin.
ACCESSION AK297879
VERSION AK297879.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2339)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2339
/clone="HEART2008787"
/clone_lib="HEART2"
/db_xref="H-InvDB:HIT000492503"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="heart"
CDS 82..2169
/codon_start=1
/note="highly similar to Calpastatin"
/protein_id="BAH12685.1"
/transl_table=1
/translation="MNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKL
SVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTK
PQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDL
IDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDA
IDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQ
ALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDK
DGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSE
AVSRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAK
EEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSG
PQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLG
QRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKK
SEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDS
AKTTEETSKPKDD"
BASE COUNT 830 a 510 c 544 g 455 t
ORIGIN
1 ggaaaccaag ccgaggactg cggggtccgg tgtccacgca gagtgtcagc ttcctctggt
61 gcaaccagca agtcttccag tatgaatccc acagaaacca aggccattcc agtcagccaa
121 cagatggaag gaccacatct tcctaacaag aaaaaacaca aaaaacaggc tgtaaaaaca
181 gaacctgaga agaagtcaca gtcaaccaag ctgtctgtgg ttcatgagaa aaaatcccaa
241 gaaggaaagc caaaagaaca cacagagcca aaaagcctac ccaagcaggc atcagataca
301 ggaagtaacg atgctcacaa taaaaaagca gtttccagat cagctgaaca gcagccatca
361 gagaaatcaa cagaaccaaa gactaaacca caagacatga tttctgctgg tggagagagt
421 gttgctggta tcactgcaat atctggcaag ccgggtgaca agaaaaaaga aaagaaatca
481 ttaaccccag ctgtgccagt tgaatctaaa ccggataaac catcgggaaa gtcaggcatg
541 gatgctgctt tggatgactt aatagatact ttaggaggac ctgaagaaac tgaagaagaa
601 aatacaacgt atactggacc agaagtttca gatccaatga gttccaccta catagaggaa
661 ttgggtaaaa gagaagtcac aattcctcca aaatataggg aactattggc taaacccata
721 gggccagatg atgctataga cgccttgtca tctgacttca cctgtgggtc gcctacagct
781 gctggaaaga aaactgaaaa agaggaatct acagaagttt taaaagctca gtcagcaggg
841 acagtcagaa gtgctgctcc accccaagag aagaaaagaa aggtggagaa ggatacaatg
901 agtgatcaag cactcgaggc tctgtcggct tcactgggca cccggcaagc agaacctgag
961 ctcgacctcc gctcaattaa ggaagtcgat gaggcaaaag ctaaagaaga aaaactagag
1021 aagtgtggtg aggatgatga aacaatccca tctgagtaca gattaaaacc agccacggat
1081 aaagatggaa aaccactatt gccagagcct gaagaaaaac ccaagcctcg gagtgaatca
1141 gaactcattg atgaactttc agaagatttt gaccggtctg aatgtaaaga gaaaccatct
1201 aagccaactg aaaagacaga agaatctaag gccgctgctc cagctcctgt gtcggaggct
1261 gtgtctcgga cctccatgtg tagtatacag tcagcacccc ctgagccggc taccttgaag
1321 ggcacagtgc cagatgatgc tgtagaagcc ttggctgata gcctggggaa aaaggaagca
1381 gatccagaag atggaaaacc tgtgatggat aaagtcaagg agaaggccaa agaagaagac
1441 cgtgaaaagc ttggtgaaaa agaagaaaca attcctcctg attatagatt agaagaggtc
1501 aaggataaag atggaaagcc actcctgcca aaagagtcta aggaacagct tccacccatg
1561 agtgaagact tccttctgga tgctttgtct gaggacttct ctggtccaca aaatgcttca
1621 tctcttaaat ttgaagatgc taaacttgct gctgccatct ctgaagtggt ttcccaaacc
1681 ccagcttcaa cgacccaagc tggagcccca ccccgtgata cctcgcagag tgacaaagac
1741 ctcgatgatg ccttggataa actctctgac agtctaggac aaaggcagcc tgacccagat
1801 gagaacaaac caatggaaga taaagtaaag gaaaaagcta aagctgaaca tagagacaag
1861 cttggagaaa gagatgacac tatcccacct gaatacagac atctcctgga tgataatgga
1921 caggacaaac cagtgaagcc acctacaaag aaatcagagg attcaaagaa acctgcagat
1981 gaccaagacc ccattgatgc tctctcagga gatctggaca gctgtccctc cactacagaa
2041 acctcacaga acacagcaaa ggataagtgc aagaaggctg cttccagctc caaagcacct
2101 aagaatggag gtaaagcgaa ggattcagca aagacaacag aggaaacttc caagccaaaa
2161 gatgactaaa gaaatacaag ttaaggtatc tggtatctgc atgtaaaatc ttcagctggt
2221 ggatggtgac ttttgaagaa caaaaggctt tggcaacaga aaacaattgt tctgggtgat
2281 ttctagaatg gtttttgttg agtctctgaa catcctaaat attggtttgt tattctttt
//