LOCUS AK297794 1892 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ55910 complete cds, highly similar to
Keratin, type II cuticular Hb6.
ACCESSION AK297794
VERSION AK297794.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1892)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1892
/cell_type="chondrocytes (HC)"
/clone="HCHON2006684"
/clone_lib="HCHON2"
/db_xref="H-InvDB:HIT000492418"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, chondrocytes"
/organism="Homo sapiens"
CDS 134..1594
/codon_start=1
/note="highly similar to Keratin, type II cuticular Hb6"
/protein_id="BAG60134.1"
/transl_table=1
/translation="MTCGSYCGGRAFSCISACGPRPGRCCITAAPYRGISCYRGLTGG
FGSHSVCGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCV
KQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKLQFYQNRECCQSNLEPLFEGYIE
TLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVSLRATAENEFVALKKDVDCA
YLRRSDLEANVEALIQEIDFLRRLYEEEIRVLQSHISDTSVVVKLDNSRDLNMDCIIA
EIKAQYDDIVTRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTA
EVENAKCQNSKLEAAVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLIREYQEV
MNSKLGLDIEIATYRRLLEGEEQRLCEGVGSVNVCVSSSRGGVVCGDLCASTTAPVVS
TRVSSVPSNSNVVVGTTNACAPSARVGVCGGSCKRC"
BASE COUNT 344 a 593 c 606 g 349 t
ORIGIN
1 agtttcgggt ggcccagcca actttcatgc cagccgtcct cagcccttcc tggtgctggg
61 tctgccaggc tcatggtcag ccaggcctgg ggccctggag aggtcctctg catcttgaat
121 tctgtggagc accatgactt gtggatctta ctgtggtggc cgcgccttca gctgcatctc
181 ggcctgcggg ccccggcccg gccgctgctg catcaccgcc gccccctacc gtggcatctc
241 ctgctaccgc ggcctcaccg ggggcttcgg cagccacagc gtgtgcggag gctttcgggc
301 cggctcctgc ggacgcagct tcggctaccg ctccgggggc gtgtgcgggc ccagtccccc
361 atgcatcacc accgtgtcgg tcaacgagag cctcctcacg cccctcaacc tggagatcga
421 ccccaacgcg cagtgcgtga agcaggagga gaaggagcag atcaagtccc tcaacagcag
481 gttcgcggcc ttcatcgaca aggtgcgctt cctggagcag cagaacaaac tgctggagac
541 aaagctgcag ttctaccaga accgcgagtg ttgccagagc aacctggagc ccctgtttga
601 gggctacatc gagactctgc ggcgggaggc cgagtgcgtg gaggccgaca gcgggaggct
661 ggcctcagag cttaaccacg tgcaggaggt gctggagggc tacaagaaga agtatgagga
721 ggaggtttct ctgagagcaa cagctgagaa cgagtttgtg gctctgaaaa aggatgtgga
781 ctgcgcctac ctccgcagat cagacctgga ggccaatgtg gaggccctga tccaggagat
841 cgacttcctg aggcggctgt atgaggagga gatccgcgtt ctccagtccc acatctcaga
901 cacctccgtg gttgtcaagc tggacaacag ccgggacctg aacatggact gcatcattgc
961 cgagatcaag gcacagtacg atgacattgt cacccgtagc cgggctgagg ccgagtcctg
1021 gtaccgcagc aagtgtgagg agatgaaggc cacggtgatc aggcacgggg agaccctgcg
1081 ccgcaccaag gaggagatca acgagctgaa ccgcatgatc cagaggctga cggccgaggt
1141 ggagaatgcc aagtgccaga attccaagct ggaggctgcg gtggctcagt ctgagcagca
1201 gggtgaggcg gccctcagcg atgcccgctg caagttggcc gagctggagg gtgccctgca
1261 gaaggccaag caggacatgg cctgcctgat cagggagtac caggaggtga tgaactccaa
1321 gctgggcctg gacatcgaga tcgccaccta caggcgcctg ctggagggcg aggagcagag
1381 gctgtgcgag ggcgtcggct cggtgaatgt ctgcgtcagc agctcccgcg gtggcgttgt
1441 ctgtggcgat ctctgcgcct ccactactgc ccctgttgtc tccaccagag tcagtagcgt
1501 ccccagcaac agcaacgtgg tggtgggcac tactaacgcc tgcgccccct ccgcccgggt
1561 tggcgtctgc ggcggcagct gtaagaggtg ctaggaggct gccgcctccg ccagcgcctg
1621 tcgccgtcac tctccaccca gccagtacct cgcgccacca gaacgcgccg cccgcgccgg
1681 cctcccagta gccgccgccc gctgcctgca ctctaagcgc cctccccacc gctccgctcc
1741 gggagccatc cccggtcgca ggagtccggg gagggccggg aggcgccatg gtctctctct
1801 gtagcctttc ctggtagtca atttgttgtc ccgaggattc atctttttct tccgcctgcc
1861 ttctgttttt tttgctgtat gcattggtct tg
//