LOCUS AK297766 2198 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ55990 complete cds, highly similar to LIM
domain and actin-binding protein 1.
ACCESSION AK297766
VERSION AK297766.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2198)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2198
/cell_type="chondrocytes (HC)"
/clone="HCHON2001565"
/clone_lib="HCHON2"
/db_xref="H-InvDB:HIT000492390"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, chondrocytes"
/organism="Homo sapiens"
CDS 14..2050
/codon_start=1
/note="highly similar to LIM domain and actin-binding
protein 1"
/protein_id="BAG60114.1"
/transl_table=1
/translation="MWQGCNCSQLSETAVDQEQLGVLTFILQRTTCLNVSADQEEQIH
PRSRLRSPPEALVQGRYPHIKDGEDLKDHSTESKKMENCLGESRHEVEKSEISENTDA
SGKIEKYNVPLNRLKMMFEKGEPTQTKILRAQSRSASGRKISENSYSLDDLEIGPGQL
SSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIK
IHKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDSQVKSEVQQPVH
PKPLSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCF
RCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEE
ILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIA
WPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSR
PFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGN
VGKTTWQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSL
NWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE"
BASE COUNT 732 a 476 c 529 g 461 t
ORIGIN
1 ggatgctttc tccatgtggc aaggctgtaa ctgttcacag ctgtctgaaa cagcagtgga
61 ccaggagcag cttggagttt taactttcat tttacaaaga acaacatgtt tgaatgtttc
121 agcagaccaa gaagaacaaa tccaccccag atctagactc aggtcacctc ctgaagccct
181 cgttcagggt cgatatcccc acatcaagga cggtgaggat cttaaagacc actcaacaga
241 aagtaaaaaa atggaaaatt gtctaggaga atccaggcat gaagtagaaa aatcagaaat
301 cagtgaaaac acagatgctt cgggcaaaat agagaaatat aatgttccgc tgaacaggct
361 taagatgatg tttgagaaag gtgaaccaac tcaaactaag attctccggg cccaaagccg
421 aagtgcaagt ggaaggaaga tctctgaaaa cagctattct ctagatgacc tggaaatagg
481 cccaggtcag ttgtcatctt ctacatttga ctcggagaaa aatgagagta gacgaaatct
541 ggaacttcca cgcctctcag aaacctctat aaaggatcga atggccaagt accaggcagc
601 tgtgtccaaa caaagcagct caaccaacta tacaaacgag ctgaaagcca gtggtggcga
661 aatcaaaatt cataaaatgg agcaaaagga gaatgtgccc ccaggtcctg aggtctgcat
721 cacccatcag gaaggggaaa agatttctgc aaatgagaat agcctggcag tccgttccac
781 ccctgccgaa gatgactccc gtgactccca ggttaagagt gaggttcaac agcctgtcca
841 tcccaagcca ctaagtccag attccagagc ctccagtctt tctgaaagtt ctcctcccaa
901 agcaatgaag aagtttcagg cacctgcaag agagacctgc gtggaatgtc agaagacagt
961 ctatccaatg gagcgtctct tggccaacca gcaggtgttt cacatcagct gcttccgttg
1021 ctcctattgc aacaacaaac tcagtctagg aacatatgca tctttacatg gaagaatcta
1081 ttgtaagcct cacttcaatc aactctttaa atctaagggc aactatgatg aaggctttgg
1141 gcacagacca cacaaggatc tatgggcaag caaaaatgaa aacgaagaga ttttggagag
1201 accagcccag cttgcaaatg caagggagac ccctcacagc ccaggggtag aagatgcccc
1261 tattgctaag gtgggtgtcc tggctgcaag tatggaagcc aaggcctcct ctcagcagga
1321 gaaggaagac aagccagctg aaaccaagaa gctgaggatc gcctggccac cccccactga
1381 acttggaagt tcaggaagtg ccttggagga agggatcaaa atgtcaaagc ccaaatggcc
1441 tcctgaagac gaaatcagca agcccgaagt tcctgaggat gtcgatctag atctgaagaa
1501 gctaagacga tcttcttcac tgaaggaaag aagccgccca ttcactgtag cagcttcatt
1561 tcaaagcacc tctgtcaaga gcccaaaaac tgtgtcccca cctatcagga aaggctggag
1621 catgtcagag cagagtgaag agtctgtggg tggaagagtt gcagaaagga aacaagtgga
1681 aaatgccaag gcttctaaga agaatgggaa tgtgggaaaa acaacctggc aaaacaaaga
1741 atctaaagga gagacaggga agagaagtaa ggaaggtcat agtttggaga tggagaatga
1801 gaatcttgta gaaaatggtg cagactccga tgaagatgat aacagcttcc tcaaacaaca
1861 atctccacaa gaacccaagt ctctgaattg gtcgagtttt gtagacaaca cctttgctga
1921 agaattcact actcagaatc agaaatccca ggatgtggaa ctctgggagg gagaagtggt
1981 caaagagctc tctgtggaag aacagataaa gagaaatcgg tattatgatg aggatgagga
2041 tgaagagtga caaattgcaa tgatgctggg ccttaaattc atgttagtgt tagcgagcca
2101 ctgccctttg tcaaaatgtg atgcacataa gcaggtatcc cagcatgaaa tgtaatttac
2161 ttggaagtaa ctttggaaaa gaattccttc ttaaaatc
//