LOCUS AK297747 1658 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ57853 complete cds, highly similar to
Rho-GTPase-activating protein 6.
ACCESSION AK297747
VERSION AK297747.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1658)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1658
/cell_type="coronary artery smooth muscle cells (HCASMC)"
/clone="HCASM2008213"
/clone_lib="HCASM2"
/db_xref="H-InvDB:HIT000492371"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, coronary artery smooth muscle
cells"
/organism="Homo sapiens"
CDS 85..1584
/codon_start=1
/note="highly similar to Rho-GTPase-activating protein 6"
/protein_id="BAG60097.1"
/transl_table=1
/translation="MPDPLLTRELYTAFINTLLLEPEEQLGTLQLLIYLLPPCNCDTL
HRLLQFLSIVARHADDNISKDGQEVTGNKMTSLNLATIFGPNLLHKQKSSDKEFSVQS
SARAEESTAIIAVVQKMIENYEALFMVPPDLQNEVLISLLETDPDVVDYLLRRKASQS
SSPDMLQSEVSFSVGGRHSSTDSNKASSGDISPYDNNSPVLSERSLLAMQEDAAPGGS
EKLYRVPGQFMLVGHLSSSKSRESSPGPRLGKDLSEEPFDIWGTWHSTLKSGSKDPGM
TGSSGDIFESSSLRAGPCSLSQGNLSPNWPRWQGSPAELDSDTQGARRTQAAAPATEG
RAHPAVSRACSTPHVQVAGKAERPTARSEQYLTLSGAHDLSESELDVAGLQSRATPQC
QRPHGSGRDDKRPPPPYPGPGKPAAAAAWIQGPPEGVETPTDQGGQAAEREQQVTQKK
LSSANSLPAGEQDSPRLGDAGWLDWQRERWQIWELLSTDNPDALPETLV"
BASE COUNT 374 a 510 c 493 g 281 t
ORIGIN
1 attgaccgtg ggattgatgt ctctctggag gaggagcaca gtgttcatga tgtggcagcc
61 ttgctgaaag agttcctgag ggacatgcca gacccccttc tcaccaggga gctgtacaca
121 gctttcatca acactctctt gttggagccg gaggaacagc tgggcacctt gcagctcctc
181 atataccttc tacctccctg caactgcgac accctccacc gcctgctaca gttcctctcc
241 atcgtggcca ggcatgccga tgacaacatc agcaaagatg ggcaagaggt cactgggaat
301 aaaatgacat ctctaaactt agccaccata tttggaccca acctgctgca caagcagaag
361 tcatcagaca aagaattctc agttcagagt tcagcccggg ctgaggagag cacggccatc
421 atcgctgttg tgcaaaagat gattgaaaat tatgaagccc tgttcatggt tcccccagat
481 ctccagaacg aagtgctgat cagcctgtta gagaccgatc ctgatgtcgt ggactattta
541 ctcagaagaa aggcttccca atcatcaagc cctgacatgc tgcagtcgga agtttccttt
601 tccgtgggag ggaggcattc atctacagac tccaacaagg cctccagcgg agacatctcc
661 ccttatgaca acaactcccc agtgctgtct gagcgctccc tgctggctat gcaagaggac
721 gcggccccgg ggggctcgga gaagctttac agagtgccag ggcagtttat gctggtgggc
781 cacttgtcgt cgtcaaagtc aagggaaagt tctcctggac caaggcttgg gaaagatctg
841 tcagaggagc ctttcgatat ctggggaact tggcattcaa cattaaaaag cggatccaaa
901 gacccaggaa tgacaggttc ctctggagac atttttgaaa gcagctccct aagagcgggg
961 ccctgctccc tttctcaagg gaacctgtcc ccaaattggc ctcggtggca ggggagcccc
1021 gcagagctgg acagcgacac gcagggggct cggaggactc aggccgcagc ccccgcgacg
1081 gagggcaggg cccaccctgc ggtgtcgcgc gcctgcagca cgccccacgt ccaggtggca
1141 gggaaagccg agcggcccac ggccaggtcg gagcagtact tgaccctgag cggcgcccac
1201 gacctcagcg agagtgagct ggatgtggcc gggctgcaga gccgggccac acctcagtgc
1261 caaagacccc atgggagtgg gagggatgac aagcggcccc cgcctccata cccgggccca
1321 gggaagcccg cggcagcggc agcctggatc caggggcccc cggaaggcgt ggagacaccc
1381 acggaccagg gaggccaagc agccgagcga gagcagcagg tcacgcagaa aaaactgagc
1441 agcgccaact ccctgccagc gggcgagcag gacagtccgc gcctggggga cgctggctgg
1501 ctcgactggc agagagagcg ctggcagatc tgggagctcc tgtcgaccga caaccccgat
1561 gccctgcccg agacgctggt ctgagcccgc acccagccga gccccccctg ccccgagccc
1621 cccgccctcc agcccagggg ggaccgtggg tggtggcc
//