LOCUS AK297719 1260 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ54270 complete cds.
ACCESSION AK297719
VERSION AK297719.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1260)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1260
/cell_type="coronary artery smooth muscle cells (HCASMC)"
/clone="HCASM2002001"
/clone_lib="HCASM2"
/db_xref="H-InvDB:HIT000492343"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, coronary artery smooth muscle
cells"
/organism="Homo sapiens"
CDS 68..490
/codon_start=1
/protein_id="BAG60073.1"
/transl_table=1
/translation="MEMSGLSFSEMEGCRNLLGLLDNDEIMALCDTVTNRLVQPQDRQ
DAVHAILAYSQSAEELLRRRKVHREVIFKYLATQGIVIPPATEKHNLIQHAKDYWQKQ
PQLKLKETPEPVTKTEDIHLFQQDHPKMNGDHSTSGMM"
BASE COUNT 384 a 274 c 275 g 327 t
ORIGIN
1 aggcgcgggc ccagtgggcc gtaggggcga cattgttgcc gtcgtctttc cccccccagt
61 cccggggatg gagatgtcgg gactcagctt ttcagagatg gagggctgcc gtaacctact
121 tggcctactg gacaacgacg agatcatggc cctatgcgac accgtcacca accgcctggt
181 gcagcctcag gaccgccaag atgctgttca tgcaatatta gcatacagtc aaagtgcaga
241 agaacttctg aggcgtagaa aagtccaccg agaagttata tttaagtact tggcaacaca
301 ggggattgtt atacctccag ctactgaaaa acacaatctt attcagcatg caaaagatta
361 ctggcaaaag caaccacaac tgaaattgaa ggaaacgcca gagccagtta caaagacaga
421 ggacatccac ctatttcaac aggaccaccc caagatgaat ggggaccaca gcacttctgg
481 catgatgtga agcttaggtt ttattacaac acatcagaac aaaatgttat ggactaccat
541 ggagcagaaa tcgtgagcct tcgtttgctg tcactagtaa aagaagaatt tctttttctc
601 agccccaacc tagattcaca tggactgaaa tgtgcatctt ctcctcatgg gctggttatg
661 gttggagttg ctgggactgt ccatcgagga aacacttgtt tgggcatttt tgaacaaatt
721 tttggactca tccgctgccc ttttgtggag aatacttgga aaatcaaatt tatcaacctg
781 aaaattatgg gagagagttc ccttgctcct ggaacattac cgaaaccatc tgttaaattt
841 gaacaaagtg atctagaggc cttttataat gtaatcactg tatgtggtac caatgaagta
901 cgacataatg taaagcaggc ttcggatagt ggaactgggg accaagtttg aggtagtgga
961 aatgagacat tgctgaacaa aagagaactg ggtttacctg accctctaaa gcgctaagta
1021 ctgtcagcct gaaaaaaatc ttctatacag aaactcttcc aaatactata tcagtaatgt
1081 ctgaatgatt tcagatgtga aaattgacat attttagttg aaataccttt ctggactaca
1141 gacttacata tcatgtgaat acttacctat ttctacccga gttgcagcaa gtattctgaa
1201 agcttaatgc aaataaatcc cactttagat cttacagcta actgtgtgcc ttagaaacca
//