LOCUS AK297713 2105 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ59725 complete cds, highly similar to
Rho-GTPase-activating protein 6.
ACCESSION AK297713
VERSION AK297713.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2105)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2105
/cell_type="coronary artery smooth muscle cells (HCASMC)"
/clone="HCASM2000290"
/clone_lib="HCASM2"
/db_xref="H-InvDB:HIT000492337"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, coronary artery smooth muscle
cells"
/organism="Homo sapiens"
CDS 73..1887
/codon_start=1
/note="highly similar to Rho-GTPase-activating protein 6"
/protein_id="BAG60069.1"
/transl_table=1
/translation="MKRLHIFGPKIHSSGGLWQQKGDFTWNSMSGRSVRLRSVPIQSL
SELERARLQEVAFYQLQQDCDLSCQITIPKDGQKRKKSLRKKLDSLGKEKNKDKEFIP
QAFGMPLSQVIANDRAYKLKQDLQRDEQKDASDFVASLLPFGNKRQNKELSSSNSSLS
STSETPNESTSPNTPEPAPRARRRGAMSVDSITDLDDNQSRLLEALQLSLPAEAQSKK
EKARDKKLSLNPIYRQVPRLVDSCCQHLEKHGLQTVGIFRVGSSKKRVRQLREEFDRG
IDVSLEEEHSVHDVAALLKEFLRDMPDPLLTRELYTAFINTLLLEPEEQLGTLQLLIY
LLPPCNCDTLHRLLQFLSIVARHADDNISKDGQEVTGNKMTSLNLATIFGPNLLHKQK
SSDKEFSVQSSARAEESTAIIAVVQKMIENYEALFMVPPDLQNEVLISLLETDPDVVD
YLLRRKASQSSSPDMLQSEVSFSVGGRHSSTDSNKASSGDISPYDNNSPVLSERSLLA
MQEDAAPGGSEKLYRVPGQFMLVGHLSSSKSRESSPGPRLGKDLSEEPFDIWGTWHST
LKSGSKDPGMTGTTPLLNISSQEDHRQKDLRLIFKIYP"
BASE COUNT 619 a 520 c 482 g 484 t
ORIGIN
1 actcatttgt gcacttactc agggacacag cctgcctctg ttctgaaagg acaggaactt
61 ctcaatgatc cgatgaaaag attgcatatt tttggtccca agattcacag tagcggaggt
121 ctctggcaac aaaagggtga tttcacctgg aacagcatgt caggccgcag tgtacggctg
181 aggtcagtcc ccatccagag tctctcagag ctggagaggg cccggctgca ggaagtggct
241 ttttatcagt tgcaacagga ctgtgacctg agctgtcaga tcaccattcc caaagatgga
301 caaaagagaa agaaatcttt aagaaagaaa ctggattcac taggaaagga gaaaaacaaa
361 gacaaagaat tcatcccaca ggcatttgga atgcccttat cccaagtcat tgcgaatgac
421 agggcctata aactcaagca ggacttgcag agggacgagc agaaagatgc atctgacttt
481 gtggcttccc tcctcccatt tggaaataaa agacaaaaca aagaactctc aagcagtaac
541 tcatctctca gctcaacctc agaaacaccg aatgagtcaa cgtccccaaa caccccggaa
601 ccggctcctc gggctaggag gaggggtgcc atgtcagtgg attctatcac cgatcttgat
661 gacaatcagt ctcgactact agaagcttta caactttcct tgcctgctga ggctcaaagt
721 aaaaaggaaa aagccagaga taagaaactc agtctgaatc ctatttacag acaggtccct
781 aggctggtgg acagctgctg tcagcaccta gaaaaacatg gcctccagac agtggggata
841 ttccgagttg gaagctcaaa aaagagagtg agacaattac gtgaggaatt tgaccgtggg
901 attgatgtct ctctggagga ggagcacagt gttcatgatg tggcagcctt gctgaaagag
961 ttcctgaggg acatgccaga cccccttctc accagggagc tgtacacagc tttcatcaac
1021 actctcttgt tggagccgga ggaacagctg ggcaccttgc agctcctcat ataccttcta
1081 cctccctgca actgcgacac cctccaccgc ctgctacagt tcctctccat cgtggccagg
1141 catgccgatg acaacatcag caaagatggg caagaggtca ctgggaataa aatgacatct
1201 ctaaacttag ccaccatatt tggacccaac ctgctgcaca agcagaagtc atcagacaaa
1261 gaattctcag ttcagagttc agcccgggct gaggagagca cggccatcat cgctgttgtg
1321 caaaagatga ttgaaaatta tgaagccctg ttcatggttc ccccagatct ccagaacgaa
1381 gtgctgatca gcctgttaga gaccgatcct gatgtcgtgg actatttact cagaagaaag
1441 gcttcccaat catcaagccc tgacatgctg cagtcggaag tttccttttc cgtgggaggg
1501 aggcattcat ctacagactc caacaaggcc tccagcggag acatctcccc ttatgacaac
1561 aactccccag tgctgtctga gcgctccctg ctggctatgc aagaggacgc ggccccgggg
1621 ggctcggaga agctttacag agtgccaggg cagtttatgc tggtgggcca cttgtcgtcg
1681 tcaaagtcaa gggaaagttc tcctggacca aggcttggga aagatctgtc agaggagcct
1741 ttcgatatct ggggaacttg gcattcaaca ttaaaaagcg gatccaaaga cccaggaatg
1801 acaggaacta ctcctctcct taacatctct tctcaagagg atcacaggca aaaagatcta
1861 aggctcattt tcaagattta cccatgatca tggagcagca ctataaatcc aaagctacta
1921 tagaagcatt gaaaacaaat ataaaaggtt caccttagtc tcattttact gagtcacagt
1981 aaaccagact tacagaatca tctattttta aggtgacaat tgttcagata gtaccactga
2041 tcaatgatcc cctaactttt tctaaacatt ttatctaaca gtgttttcag ttttttaaaa
2101 aacct
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