LOCUS AK297240 2281 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ52328 complete cds, highly similar to Mus
musculus kelch-like 32 (Drosophila) (Klhl32), mRNA.
ACCESSION AK297240
VERSION AK297240.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2281)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2281
/clone="FCBBF3002939"
/clone_lib="FCBBF3"
/db_xref="H-InvDB:HIT000491864"
/db_xref="taxon:9606"
/dev_stage="fetal"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="brain"
CDS 217..1872
/codon_start=1
/note="highly similar to Mus musculus kelch-like 32
(Drosophila) (Klhl32), mRNA"
/protein_id="BAH12528.1"
/transl_table=1
/translation="MPSERCLSIQEMLTGQRLCHSESHNDSVLAALNQQRSDGILCDI
TLIAEEQKFHAHKAVLAACSDYFRELNSFNYLDLYRLADLFNLTLLEKAVIDFLVKHL
SELLKSRPEEVLTLPYCLLQEVLKSDRLTSLSEEQIWQLAVRWLEHNCHYQYMDELLQ
YIRFGLMDVDTLHTVALSHPLVQASETATALVNEALEYHQSIYAQPVWQTRRTKPRFQ
SDTLYIIGGKKREVCKVKELRYFNPVDQENALIAAIANWSELAPMPVGRSHHCVAVMG
DFLFVAGGEVEHASGRTCAVRTACRYDPRSNSWAEIAPMKNCREHFVLGAMEEYLYAV
GGRNELRQVLPTVERYCPKKNKWTFVQSFDRSLSCHAGYVADGLLWISGGVTNTAQYQ
NRLMVYEPNQNKWISRSPMLQRRVYHSMAAVQRKLYVLGGNDLDYNNDRILVRHIDSY
NIDTDQWTRCNFNLLTGQNESGVAVHNGRIYLVGGYSIWTNEPLACIQVLDVSREGKE
EVFYGPTLPFASNGIAACFLPAPYFTCPNLQTLQVPHHRIGTI"
BASE COUNT 604 a 545 c 541 g 591 t
ORIGIN
1 actgcatccc cgaaccagca gagcgaagct actgcgggtt ctgttaacct cagcatcgtg
61 gggcgaagca gagccattgt gcatcaagga gaggccggtg cctgcgctgc cgtctctggc
121 acctaaccca gcagaccgct caccccatcg gctggaatgc ttgctgattc ctctgcacac
181 ttctaaggct gagaacctga ggaacccagc tggaaaatgc cgtctgaacg ctgcctcagt
241 attcaagaaa tgctgacagg ccagaggctc tgccactccg aatctcacaa tgacagtgtc
301 ctggcagcgc tgaatcagca gaggagtgat ggcatcctct gcgacatcac cctgattgct
361 gaggaacaga aattccatgc tcacaaggca gtcctagcag catgcagtga ctatttccgg
421 gaattaaata gctttaatta cttggatctg tacagacttg ctgacctctt taacctcact
481 ttgttggaga aggcagtgat cgatttctta gtgaaacatc tctctgaact cctgaagagc
541 cgcccagaag aagttctaac gcttccctat tgcctgcttc aggaggtgct gaagagcgac
601 cgcctgacct ccctgagtga agagcagatc tggcagctag ctgtgaggtg gttggaacac
661 aactgccact accagtacat ggacgagctc ctgcaataca tccgctttgg cctaatggat
721 gtggatactc tccatacagt tgccctgtcc cacccccttg tccaagcaag tgagactgca
781 acagcccttg tcaacgaggc cctggaatac caccagagca tctatgcaca gcctgtctgg
841 cagactcgca ggaccaaacc acgattccag tcagacactc tgtatatcat tggtgggaaa
901 aagcgcgagg tctgcaaggt caaggaactt cggtacttca atcctgttga tcaggagaat
961 gctctcatag ctgccattgc caactggagt gagctggctc ccatgcctgt ggggaggagc
1021 caccattgtg tggcagtcat gggggacttc ctgtttgtgg caggagggga agttgagcat
1081 gccagtggcc ggacgtgtgc tgtgaggact gcctgtcgct atgacccccg cagtaattcc
1141 tgggcagaga tagcacccat gaaaaactgc cgggagcatt ttgtgctggg tgccatggag
1201 gaatacctct atgcagttgg gggcagaaat gaactgcgcc aggttctgcc tacagttgag
1261 cgatattgcc ccaagaagaa caaatggact tttgttcagt cctttgacag atccctttca
1321 tgccatgctg gatatgtggc tgatggtctt ctttggatat caggtggagt aactaatacg
1381 gcacaatatc agaacaggct aatggtgtat gaacctaacc agaataagtg gataagccgt
1441 agccccatgc tgcagagaag ggtctaccat tccatggctg ctgtacaaag gaagctttat
1501 gttcttggag gcaatgacct agactacaat aatgaccgga tccttgtgcg ccatatagat
1561 tcttacaaca tagacactga ccagtggaca cgttgtaatt tcaacctgct gactggccaa
1621 aatgaatctg gagttgctgt ccataatggg agaatatatt tagttggtgg atattcaatt
1681 tggacaaatg agcctctggc ttgtatccag gtactggatg taagcagaga aggcaaagaa
1741 gaagtattct atgggcctac actccctttt gcttccaatg gaatagcagc atgcttcctt
1801 ccagctccat attttacatg ccctaacctt caaactcttc aagtgcctca tcacaggatt
1861 ggcaccatct gaaaagccaa gccatcatga acaggaggaa aacatagctc tgactgttgg
1921 atactgggca tgaaaagact cagtgctcca tgcttccttg tcttgcttta taggtcttat
1981 attcggataa atttaagcaa aaaatgaaca attttctaaa atacgttatt gaaaactcgt
2041 cacccttctc agtgtatgtc aacattcaat atgtatgact tttattgtgg tatagcttag
2101 tgccaattta aggtatattg tgttccatgg gtctttagag cattctttgt acactttttc
2161 taatcagcac tgtcttaaga caacataaca ctgttagtaa ggcaatttat tggacacaat
2221 ggcatcgatg atcttaaaaa tatatatatt ctgtacttaa tccctttatt atttaaagcc
2281 g
//