LOCUS AK297205 2382 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ57031 complete cds, highly similar to
Midline-1 (EC 6.3.2.-).
ACCESSION AK297205
VERSION AK297205.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2382)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2382
/clone="FCBBF3000265"
/clone_lib="FCBBF3"
/db_xref="H-InvDB:HIT000491829"
/db_xref="taxon:9606"
/dev_stage="fetal"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="brain"
CDS 348..2201
/codon_start=1
/note="highly similar to Midline-1 (EC 6.3.2.-)"
/protein_id="BAG59690.1"
/transl_table=1
/translation="METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCAT
NESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETSA
EKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRG
LMCLEHEDEKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDL
LIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHA
RFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNP
PTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPN
IKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVS
HDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLA
YKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIA
FYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP"
BASE COUNT 621 a 637 c 579 g 545 t
ORIGIN
1 ctgatttgaa ataagagcca ggcggtcctc gcttccctgc gaccgacttt tcataggctg
61 ggggagaaaa ggttgagaaa cttgacattg tctcgagcag agtgcgtgta gcaacagatc
121 aaagaaaaag aggacgaaaa cgtgctcttt gctgcccgta gatttcgccg ggttgctttt
181 gtcttgcggg ctcctgtcgg gttcggtgtt tccgctctga agactgcgac gcgggctccg
241 atgcagctcg ctccctgccg gatgggtcat gggattctaa acatgaggca gatagctgat
301 cagcttcctt gggttttgct gatgacacaa gagagctttg cctgaagatg gaaacactgg
361 agtcagaact gacctgccct atttgtctgg agctctttga ggaccctctt ctactgccct
421 gcgcacacag cctctgcttc aactgcgccc accgcatcct agtatcacac tgtgccacca
481 acgagtctgt ggagtccatc accgccttcc agtgccccac ctgccggcat gtcatcaccc
541 tcagccagcg aggtctagac gggctcaagc gcaacgtcac cctacagaac atcatcgaca
601 ggttccagaa agcatcagtg agcgggccca actctcccag cgagacctcc gccgagaagg
661 tcctctgcca gttttgtgac caggatcctg cccaggacgc tgtgaagacc tgtgtcactt
721 gtgaagtatc ctactgtgac gagtgcctga aagccactca cccgaataag aagcccttta
781 caggccatcg tctgattgag ccaattccgg actctcacat ccgggggctg atgtgcttgg
841 agcatgagga tgagaagcaa aacttagaga gtaacctcac caaccttatt aagaggaaca
901 cagaactgga gacccttttg gctaaactca tccaaacctg tcaacatgtt gaagtcaatg
961 catcacgtca agaagccaaa ttgacagagg agtgtgatct tctcattgag atcattcagc
1021 aaagacgaca gattattgga accaagatca aagaagggaa ggtgatgagg cttcgcaaac
1081 tggctcagca gattgcaaac tgcaaacagt gcattgagcg gtcagcatca ctcatctccc
1141 aagcggaaca ctctctgaag gagaatgatc atgcgcgttt cctacagact gctaagaata
1201 tcaccgagag agtctccatg gcaactgcat cctcccaggt tctaattcct gaaatcaacc
1261 tcaatgacac atttgacacc tttgccttag atttttcccg agagaagaaa ctgctagaat
1321 gtctggatta ccttacagct cccaaccctc ccacaattag agaagagctc tgcacagctt
1381 catatgacac catcactgtg cattggacct ccgatgatga gttcagcgtg gtctcctacg
1441 agctccagta caccatattc accggacaag ccaacgtcgt tagtctgtgt aattcggctg
1501 atagctggat gatagtaccc aacatcaagc agaaccacta cacggtgcac ggtctgcaga
1561 gcggcaccaa gtacatcttc atggtcaagg ccatcaacca ggcgggcagc cgcagcagtg
1621 agcctgggaa gttgaagaca aacagccaac catttaaact ggatcccaaa tctgctcatc
1681 gaaaactgaa ggtgtcccat gataacttga cagtagaacg tgatgagtca tcatccaaga
1741 agagtcacac acctgaacgc ttcaccagcc aggggagcta tggagtagct ggaaatgtgt
1801 ttattgatag tggccggcat tattgggaag tggtcataag tggaagcaca tggtatgcca
1861 ttggtcttgc ttacaaatca gccccgaagc atgaatggat tgggaagaac tctgcttcct
1921 gggcgctctg ccgctgcaac aataactggg tggtgagaca caatagcaag gaaatcccca
1981 ttgagcctgc cccccacctc cggcgcgtgg gcatcctgct ggactatgat aacggctcta
2041 tcgcctttta tgatgctttg aactccatcc acctctacac cttcgacgtc gcatttgcgc
2101 agcctgtttg ccccaccttc accgtgtgga acaagtgtct gacgattatc actgggctcc
2161 ctatcccaga ccatttggac tgcacagagc agctgccgtg agcgtctggc cacatggagc
2221 tgctttctgg ggaacagtaa ggttcaggcc actatttagg ggactgagaa agcacaggct
2281 tcatgagtgt aatgaaatct caccagaagt gtcccgaaat cggctcagat agggctcaaa
2341 acaagagatt cctctccttt tactgtgtct tgtattaagt ac
//