LOCUS AK297171 2061 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ50986 complete cds, highly similar to DNA
topoisomerase 2-beta (EC 5.99.1.3).
ACCESSION AK297171
VERSION AK297171.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2061)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2061
/clone="FCBBF2002191"
/clone_lib="FCBBF2"
/db_xref="H-InvDB:HIT000491795"
/db_xref="taxon:9606"
/dev_stage="fetal"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="brain"
CDS 302..1738
/codon_start=1
/note="highly similar to DNA topoisomerase 2-beta (EC
5.99.1.3)"
/protein_id="BAG59664.1"
/transl_table=1
/translation="MSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAF
VEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEITAMK
ADASKKLLKKKKGDLDTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINKGPKPKREKK
EPGTRVRKTPTSSGKPSAKKVKKRNPWSDDESKSESDLEETEPVVIPRDSLLRRAAAE
RPKYTFDFSEEEDDDADDDDDDNNDLEELKVKASPITNDGEDEFVPSDGLDKDEYTFS
PGKSKATPEKSLHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDSASVFSPSFGLK
QTDKVPSKTVAAKKGKPSSDTVPKPKRAPKQKKVVEAVNSDSDSEFGIPKKTTTPKGK
GRGAKKRKASGSENEGDYNPGRKTSKTTSKKPKKTSFDQDSDVDIFPSDFPTEPPSLP
RTGRARKEVKYFAESDEEEDDVDFAMFN"
BASE COUNT 717 a 340 c 451 g 553 t
ORIGIN
1 tgtaattcca tggtactttt tgatcatatg ggatgtctga agaaatatga aactgtgcaa
61 gacattctga aagaattctt tgatttacga ttaagttatt acggtttacg taaggagtgg
121 cttgtgggaa tgttgggagc agaatctaca aagcttaaca atcaagcccg tttcatttta
181 gagaagatac aagggaaaat tactataggc agcagaagag gatgaaacac aaaaccagca
241 tgatgatagt tcctccgatt caggaactcc ttcaggccca gattttaatt atattttaaa
301 tatgtctctg tggtctctta ctaaagaaaa agttgaagaa ctgattaaac agagagatgc
361 aaaagggcga gaggtcaatg atcttaaaag aaaatctcct tcagatcttt ggaaagagga
421 tttagcggca tttgttgaag aactggataa agtggaatct caagaacgag aagatgttct
481 ggctggaatg tctggaaaag caattaaagg taaagttggc aaacctaagg tgaagaaact
541 ccagttggaa gagacaatgc cctcacctta tggcagaaga ataattcctg aaattacagc
601 tatgaaggca gatgccagca aaaagttgct gaagaagaag aagggtgatc ttgatactgc
661 agcagtaaaa gtggaatttg atgaagaatt cagtggagca ccagtagaag gtgcaggaga
721 agaggcattg actccatcag ttcctataaa taaaggtccc aaacctaaga gggagaagaa
781 ggagcctggt accagagtga gaaaaacacc tacatcatct ggtaaaccta gtgcaaagaa
841 agtgaagaaa cggaatcctt ggtcagatga tgaatccaag tcagaaagtg atttggaaga
901 aacagaacct gtggttattc caagagattc tttgcttagg agagcagcag ccgaaagacc
961 taaatacaca tttgatttct cagaagaaga ggatgatgat gctgatgatg atgatgatga
1021 caataatgat ttagaggaat tgaaagttaa agcatctccc ataacaaatg atggggaaga
1081 tgaatttgtt ccttcagatg ggttagataa agatgaatat acattttcac caggcaaatc
1141 aaaagccact ccagaaaaat ctttgcatga caaaaaaagt caggattttg gaaatctctt
1201 ctcatttcct tcatattctc agaagtcaga agatgattca gctaaatttg acagtaatga
1261 agaagattct gcttctgttt tttcaccatc atttggtctg aaacagacag ataaagttcc
1321 aagtaaaacg gtagctgcta aaaagggaaa accgtcttca gatacagtcc ctaagcccaa
1381 gagagcccca aaacagaaga aagtagtaga ggctgtaaac tctgactcgg attcagaatt
1441 tggcattcca aagaagacta caacaccaaa aggtaaaggc cgaggggcaa agaaaaggaa
1501 agcatctggc tctgaaaatg aaggcgatta taaccctggc aggaagacat ccaaaacaac
1561 aagcaagaaa ccgaagaaga catcttttga tcaggattca gatgtggaca tcttcccctc
1621 agacttccct actgagccac cttctctgcc acgaaccggt cgggctagga aagaagtaaa
1681 atattttgca gagtctgatg aagaagaaga tgatgttgat tttgcaatgt ttaattaagt
1741 gcccaaagag cacaaacatt tttcaacaaa tatcttgtgt tgtccttttg tcttctctgt
1801 ctcagacttt tgtacatctg gcttatttta atgtgatgat gtaattgacg gttttttatt
1861 attgtggtag gccttttaac attttgttct tacacataca gttttatgct cttttttact
1921 cattgaaatg tcacgtactg tctgattggc ttgtagaatt gttatagact gccgtgcatt
1981 agcacagatt ttaattgtca tggttacaaa ctacagacct gctttttgaa atgaaattta
2041 aacattaaaa atggaactgt g
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