LOCUS AK297147 2046 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ53847 complete cds, highly similar to
Ribosomal protein S6 kinase beta-1 (EC 2.7.11.1).
ACCESSION AK297147
VERSION AK297147.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2046)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2046
/cell_line="TF-1"
/cell_type="erythroleukemia"
/clone="ERLTF1000010"
/clone_lib="ERLTF1"
/db_xref="H-InvDB:HIT000491771"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 235..1653
/codon_start=1
/note="highly similar to Ribosomal protein S6 kinase
beta-1 (EC 2.7.11.1)"
/protein_id="BAG59646.1"
/transl_table=1
/translation="MDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVL
GKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFI
VDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKG
IIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHN
RAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLK
RNAASRLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFT
RQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTP
VSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNS
GPYKKQAFPMISKRPEHLRMNL"
BASE COUNT 650 a 401 c 493 g 502 t
ORIGIN
1 agggaagctg aggacatggc aggagcgttt gacatagacc tggaccagcc agaggacgcg
61 ggctctgagg atgagctgga ggagggggaa gcgctcagct gttagaagtg acagctgaaa
121 acttctgtcg ggagtagcac tgccgctgct gttacagcca ccaggagttt tatttcggga
181 gcaagggggc tctgctgcat cttccaatct tccaggggtc agttaaatga aagcatggac
241 catgggggag ttggaccata tgaacttggc atggaacatt gtgagaaatt tgaaatctca
301 gaaactagtg tgaacagagg gccagaaaaa atcagaccag aatgttttga gctacttcgg
361 gtacttggta aagggggcta tggaaaggtt tttcaagtac gaaaagtaac aggagcaaat
421 actgggaaaa tatttgccat gaaggtgctt aaaaaggcaa tgatagtaag aaatgctaaa
481 gatacagctc atacaaaagc agaacggaat attctggagg aagtaaagca tcccttcatc
541 gtggatttaa tttatgcctt tcagactggt ggaaaactct acctcatcct tgagtatctc
601 agtggaggag aactatttat gcagttagaa agagagggaa tatttatgga agacactgcc
661 tgcttttact tggcagaaat ctccatggct ttggggcatt tacatcaaaa ggggatcatc
721 tacagagacc tgaagccgga gaatatcatg cttaatcacc aaggtcatgt gaaactaaca
781 gactttggac tatgcaaaga atctattcat gatggaacag tcacacacac attttgtgga
841 acaatagaat acatggcccc tgaaatcttg atgagaagtg gccacaatcg tgctgtggat
901 tggtggagtt tgggagcatt aatgtatgac atgctgactg gagcaccccc attcactggg
961 gagaatagaa agaaaacaat tgacaaaatc ctcaaatgta aactcaattt gcctccctac
1021 ctcacacaag aagccagaga tctgcttaaa aagctgctga aaagaaatgc tgcttctcgt
1081 ctgggagctg gtcctgggga cgctggagaa gttcaagctc atccattctt tagacacatt
1141 aactgggaag aacttctggc tcgaaaggtg gagcccccct ttaaacctct gttgcaatct
1201 gaagaggatg taagtcagtt tgattccaag tttacacgtc agacacctgt cgacagccca
1261 gatgactcaa ctctcagtga aagtgccaat caggtctttc tgggttttac atatgtggct
1321 ccatctgtac ttgaaagtgt gaaagaaaag ttttcctttg aaccaaaaat ccgatcacct
1381 cgaagattta ttggcagccc acgaacacct gtcagcccag tcaaattttc tcctggggat
1441 ttctggggaa gaggtgcttc ggccagcaca gcaaatcctc agacacctgt ggaataccca
1501 atggaaacaa gtggcataga gcagatggat gtgacaatga gtggggaagc atcggcacca
1561 cttccaatac gacagccgaa ctctgggcca tacaaaaaac aagcttttcc catgatctcc
1621 aaacggccag agcacctgcg tatgaatcta tgacagagca atgcttttaa tgaatttaag
1681 gcaaaaaagg tggagaggga gatgtgtgag catcctgcaa ggtgaaacga ctcaaaatga
1741 cagtttcaga gagtcaatgt cattacatag aacacttcag acacaggaaa aataaacgtg
1801 gattttaaaa aatcaatcaa tggtgcaaaa aaaaacttaa agcaaaatag tattgctgaa
1861 ctcttaggca catcaattaa ttgattcctc gcgacatctt ctcaacctta tcaaggattt
1921 tcatgttgat gactcgaaac tgacagtatt aagggtagga tgttgcttct gaatcactgt
1981 tgagttctga ttgtgttgaa gaagggttat cctttcatta ggcaaagtac aaaattgcct
2041 ataata
//