LOCUS AK297120 2126 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ55280 complete cds, highly similar to Heat
shock 70 kDa protein 12B.
ACCESSION AK297120
VERSION AK297120.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2126)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2126
/cell_type="normal dermal fibroblasts (Neonatal Skin)
(NHDF2564)"
/clone="DFNES2005803"
/clone_lib="DFNES2"
/db_xref="H-InvDB:HIT000491744"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal dermal fibroblasts"
/organism="Homo sapiens"
CDS 434..1996
/codon_start=1
/note="highly similar to Heat shock 70 kDa protein 12B"
/protein_id="BAG59626.1"
/transl_table=1
/translation="MPALEVFAHALRFFREHALQELREQSPSLPEKDTVRWVLTVPAI
WKQPAKQFMREAAYLAGLVSRENAEQLLIALEPEAASVYCRKLRLHQLLDLSGRAPGG
GRLGERRSIDSSFRQAREQLRRSRHSRTFLVESGVGELWAEMQAGDRYVVADCGGGTV
DLTVHQLEQPHGTLKELYKASGGPYGAVGVDLAFEQLLCRIFGEDFIATFKRQRPAAW
VDLTIAFEARKRTAGPHRAGALNISLPFSFIDFYRKQRGHNVETALRRSSVNFVKWSS
QGMLRMSCEAMNELFQPTVSGIIQHIEALLARPEVQGVKLLFLVGGFAESAVLQHAVQ
AALGARGLRVVVPHDVGLTILKGAVLFGQAPGVVRVRRSPLTYGVGVLNRFVPGRHPP
EKLLVRDGRRWCTDVFERFVAAEQSVALGEEVRRSYCPARPGQRRVLINLYCCAAEDA
RFITDPGVRKCGALSLELEPADCGQDTAGAPPGRREIRAAMQFGDTEIKVTAVDVSTN
RSVRASIDFLSN"
BASE COUNT 343 a 707 c 730 g 346 t
ORIGIN
1 ggaggcctca gggccgggcg cacgtcgagg gctgcggccg ccgcagcggg cacggccaac
61 gagctgcggg cccgggatcg cggcggctgg acggggctgg agctgtcggg agggcggagc
121 tacagggcct gcaaggatgt tggctgtccc ggagatgggc ctgcaggggc tgtacatcgg
181 aaatgggagg gcggagaccc gggcgtggcc caccagaaga ccccgacctg cctgctgctg
241 actccggagg gcgccttcca cagctttggc tacaccgccc gcgattacta ccatgacctg
301 gaccccgaag aggcgcggga ctggctctac ttcgagaagt tcaagatgaa gatccacagc
361 gccacgagcc gccctgtgtc cctgccagga tctcaccttg aagacccagc tagaggcagt
421 aaatggaaag acgatgcccg ccctggaggt gttcgcccat gccctgcgct tcttcaggga
481 gcacgccctt caggagctga gggagcagag cccatcgctg ccagagaagg acactgtgcg
541 ctgggtgttg acggtgcctg ccatctggaa acagccagcc aagcagttca tgcgggaggc
601 tgcctacctg gctggactag tgtcccgaga gaatgcagag cagctactca tcgccctgga
661 gcccgaggcc gcctcggtat actgccgcaa gctgcgcctg caccagctcc tggacctgag
721 tggccgggcc ccaggtggtg ggcgcctggg tgagcgccgc tccatcgact ccagcttccg
781 tcaggctcgg gagcagctgc gaaggtcccg ccacagccgc acgttcctgg tggagtcagg
841 cgtaggagag ctgtgggcag agatgcaagc aggagaccgc tacgtggtgg ccgactgcgg
901 cggaggcacc gtggacctga cggtgcacca gctggagcag ccccatggca ccctcaagga
961 gctctacaag gcatctgggg gcccttatgg cgcggtgggc gtggacctgg ccttcgagca
1021 gctgctgtgc cgcatcttcg gcgaggactt catcgccacc ttcaaaaggc aacggccggc
1081 agcctgggta gatctgacca tcgccttcga ggctcgcaag cgcactgctg gcccacaccg
1141 tgcaggggcg ctcaacatct cgctgccctt ctccttcatt gacttctacc gcaagcagcg
1201 gggccacaac gtggagaccg ctctgcgcag gagcagcgtg aacttcgtga agtggtcctc
1261 acaggggatg ctccgaatgt cttgtgaagc catgaacgag ctctttcagc ccaccgtcag
1321 cgggatcatc cagcacatag aggccctgct ggcacggccg gaggtgcagg gtgtgaagct
1381 gctgttccta gtgggcggct tcgccgagtc agcggtgctg cagcacgcgg tgcaggcggc
1441 gctgggcgcc cgcggtctgc gtgtcgtggt cccgcacgac gtgggcctca ccatcctcaa
1501 aggcgcggtg ctgttcggcc aggcgccggg cgtggtgcgg gtccgccgct cgccgctcac
1561 ctatggcgtg ggcgtgctca accgctttgt gcctgggcgc cacccgcccg aaaagctgct
1621 ggttcgcgac ggccgccgct ggtgcaccga cgtcttcgag cgcttcgtgg ccgccgagca
1681 gtcggtggcc ctgggcgagg aggtgcggcg cagctactgc ccggcgcgtc ccggccagcg
1741 gcgcgtactc atcaacctgt actgctgcgc ggcagaggat gcgcgcttca tcaccgaccc
1801 cggcgtgcgc aaatgtggcg cgctcagcct cgagcttgag cccgccgact gcggccagga
1861 caccgccggc gcgcctcccg gccgccgcga gatccgcgcc gccatgcagt ttggcgacac
1921 cgaaattaag gtcaccgccg tcgacgtcag caccaatcgc tccgtgcgcg cgtccatcga
1981 ctttctttcc aactgagggc gcgccggcgc ggtgccagcg ccgtctgccc ggccccgccc
2041 tctttcggtt caggggcctg cggagcgggt tggggcgggg gaaacgatag ttctgcagtc
2101 tgcgcctttc cacgccctcc agcccc
//