LOCUS AK297057 1954 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ50571 complete cds, highly similar to
Myotubularin (EC 3.1.3.48).
ACCESSION AK297057
VERSION AK297057.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1954)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1954
/clone="D9OST2000471"
/clone_lib="D9OST2"
/db_xref="H-InvDB:HIT000491681"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from CD34+ cells after 9-days ODF induction."
/note="primary culture, CD34+ cells"
/organism="Homo sapiens"
/tissue_type="cord blood"
CDS 192..1658
/codon_start=1
/note="highly similar to Myotubularin (EC 3.1.3.48)"
/protein_id="BAH12485.1"
/transl_table=1
/translation="MRNLRFALKQEGHSRRDMFEILTRYAFPLAHSLPLFAFLNEEKF
NVDGWTVYNPVEEYRRQGLPNHHWRITFINKCYELCDTYPALLVVPYRASDDDLRRVA
TFRSRNRIPVLSWIHPENKTVIVRCSQPLVGMSGKRNKDDEKYLDVIRETNKQISKLT
IYDARPSVNAVANKATGGGYESDDAYHNAELFFLDIHNIHVMRESLKKVKDIVYPNVE
ESHWLSSLESTHWLEHIKLVLTGAIQVADKVSSGKSSVLVHCSDGWDRTAQLTSLAML
MLDSFYRSIEGFEILVQKEWISFGHKFASRIGHGDKNHTDADRSPIFLQFIDCVWQMS
KQFPTAFEFNEQFLIIILDHLYSCRFGTFLFNCESARERQKVTERTVSLWSLINSNKE
KFKNPFYTKEINRVLYPVASMRHLELWVNYYIRWNPRIKQQQPNPVEQRYMELLALRD
EYIKRLEELQLANSAKLSDPPTSPSSPSQMMPHVQTHF"
BASE COUNT 597 a 388 c 431 g 538 t
ORIGIN
1 agccgagcag cctggcaacg gcggtggcgc ccggagcccg agagtttcca ggatggcttc
61 tgcatcaact tctaaatata attcacactc cttggagaat gagtctatta agaggacgtc
121 tcgagatgga gtcaatcgag gtctcactga ggctgttcct cgacttccag gagaaacact
181 aatcactgga catgagaaac ctgaggttcg ctttgaaaca ggaaggccac agcagaagag
241 atatgtttga gatcctcacg agatacgcgt ttcccctggc tcacagtctg ccattatttg
301 catttttaaa tgaagaaaag tttaacgtgg atggatggac agtttacaat ccagtggaag
361 aatacaggag gcagggcttg cccaatcacc attggagaat aacttttatt aataagtgct
421 atgagctctg tgacacttac cctgctcttt tggtggttcc gtatcgtgcc tcagatgatg
481 acctccggag agttgcaact tttaggtccc gaaatcgaat tccagtgctg tcatggattc
541 atccagaaaa taagacggtc attgtgcgtt gcagtcagcc tcttgtcggt atgagtggga
601 aacgaaataa agatgatgag aaatatctcg atgttatcag ggagactaat aaacaaattt
661 ctaaactcac catttatgat gcaagaccca gcgtaaatgc agtggccaac aaggcaacag
721 gaggaggata tgaaagtgat gatgcatatc ataacgccga acttttcttc ttagacattc
781 ataatattca tgttatgcgg gaatctttaa aaaaagtgaa ggacattgtt tatcctaatg
841 tagaagaatc tcattggttg tccagtttgg agtctactca ttggttagaa catatcaagc
901 tcgttttgac aggagccatt caagtagcag acaaagtttc ttcagggaag agttcagtgc
961 ttgtgcattg cagtgacgga tgggacagga ctgctcagct gacatccttg gccatgctga
1021 tgttggatag cttctatagg agcattgaag ggttcgaaat actggtacaa aaagaatgga
1081 taagttttgg acataaattt gcatctcgaa taggtcatgg tgataaaaac cacaccgatg
1141 ctgaccgttc tcctattttt ctccagttta ttgattgtgt gtggcaaatg tcaaaacagt
1201 tccctacagc ttttgaattc aatgaacaat ttttgattat aattttggat catctgtata
1261 gttgccgatt tggtactttc ttattcaact gtgaatctgc tcgagaaaga cagaaggtta
1321 cagaaaggac tgtttcttta tggtcactga taaacagtaa taaagaaaaa ttcaaaaacc
1381 ccttctatac taaagaaatc aatcgagttt tatatccagt tgccagtatg cgtcacttgg
1441 aactctgggt gaattactac attagatgga accccaggat caagcaacaa cagccgaatc
1501 cagtggagca gcgttacatg gagctcttag ccttacgcga cgaatacata aagcggcttg
1561 aggaactgca gctcgccaac tctgccaagc tttctgatcc cccaacttca ccttccagtc
1621 cttcgcaaat gatgccccat gtgcaaactc acttctgagg ggggaccctg gcaccgcatt
1681 agagctcgaa ataaaggcga tagctgactt tcatttgggg catttgtaaa aagtagatta
1741 aaatatttgc ctccatgtag aacttgaact aacataatct taaactcttg aatatgtgcc
1801 ttctagaata catattacaa gaaaactaca gggtccacac ggcaatcaga agaaaggagc
1861 tgagatgagg ttttggaaaa ccctgacacc tttaaaaagc agtttttgaa agacaaaatt
1921 tagatttaat ttacgtcttg agaaatacta tata
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