LOCUS AK297033 1824 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ51892 complete cds, highly similar to
Biotinidase precursor (EC 3.5.1.12).
ACCESSION AK297033
VERSION AK297033.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1824)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1824
/clone="D3OST2003973"
/clone_lib="D3OST2"
/db_xref="H-InvDB:HIT000491657"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from CD34+ cells after 3-days ODF induction."
/note="primary culture, CD34+ cells"
/organism="Homo sapiens"
/tissue_type="cord blood"
CDS 93..1730
/codon_start=1
/note="highly similar to Biotinidase precursor (EC
3.5.1.12)"
/protein_id="BAG59561.1"
/transl_table=1
/translation="MPEGGGTSRRLLPMQSRFVVCIMSGARSKLALFLCGCYVVALGA
HTGEESVADHHEAEYYVAAVYEHPSILSLNPLALISRQEALELMNQNLDIYEQQVMTA
AQKDVQIIVFPEDGIHGFNFTRTSIYPFLDFMPSPQVVRWNPCLEPHRFNDTEVLQRL
SCMAIRGDMFLVANLGTKEPCHSSDPRCPKDGRYQFNTNVVFSNNGTLVDRYRKHNLY
FEAAFDVPLKVDLITFDTPFAGRFGIFTCFDILFFDPAIRVLRDYKVKHVVYPTAWMN
QLPLLAAIEIQKAFAVAFGINVLAANVHHPVLGMTGSGIHTPLESFWYHDMENPKSHL
IIAQVAKNPVGLIGAENATGETDPSHSKFLKILSGDPYCEKDAQEVHCDEATKWNVNA
PPTFHSEMMYDNFTLVPVWGKEGYLHVCSNGLCCYLLYERPTLSKELYALGVFDGLHT
VHGTYYIQVCALVRCGGLGFDTCGQEITEATGIFEFHLWGNFSTSYIFPLFLTSGMTL
EVPDQLGWENDHYFLRKSRLSSGLVTAALYGRLYERD"
BASE COUNT 428 a 459 c 464 g 473 t
ORIGIN
1 attacacttc ccgggagagg tgagaatgta aacacgcgcg ttctccaatc agaactgcgc
61 tctcttctcg gctcctccat tcgcgcgcca gaatgccaga gggaggcggg actagcagga
121 gattgctgcc tatgcaaagc agatttgtgg tctgcattat gtctggagcc agaagtaagc
181 ttgctctttt cctctgcggc tgttacgtgg ttgccctggg agcccacacc ggggaggaga
241 gcgtggctga ccatcacgag gctgaatatt atgtggctgc cgtgtatgag catccatcca
301 tcctgagtct gaaccctctg gctctcatca gccgccaaga ggccttggag ctcatgaacc
361 agaaccttga catctatgaa cagcaagtga tgactgcagc ccaaaaggat gtacagatta
421 tagtgtttcc agaagatggc attcatggat tcaactttac aagaacatcc atttatccat
481 ttttggactt catgccgtct ccccaggtgg tcaggtggaa cccatgcctg gagcctcacc
541 gcttcaatga cacagaggtg ctccagcgcc tgagttgtat ggccatcagg ggagatatgt
601 tcttggtggc caatcttggg acaaaggagc cttgtcatag cagtgaccca aggtgcccaa
661 aagatgggag ataccagttc aacacaaatg tcgtgttcag caataatgga acccttgttg
721 accgctaccg taaacacaac ctctactttg aggcagcatt cgatgttcct cttaaagtgg
781 atctcatcac ctttgatacc ccctttgctg gcaggtttgg catcttcaca tgctttgata
841 tattgttctt tgaccctgcc atcagagtcc tcagagacta caaggtgaag catgttgtgt
901 acccaactgc ctggatgaac cagctcccac tcttggcagc aattgagatt cagaaagctt
961 ttgctgttgc ctttggcatc aacgttctgg cagctaatgt ccaccaccca gttctgggga
1021 tgacaggaag tggcatacac acccctctgg agtccttttg gtaccatgac atggaaaatc
1081 ccaaaagtca ccttataatt gcccaggtgg ccaaaaatcc agtgggtctc attggtgcag
1141 agaatgcaac aggtgaaacg gacccatccc atagtaagtt tttaaaaatt ttgtcaggcg
1201 atccgtactg tgagaaggat gctcaggaag tccactgtga tgaggccacc aagtggaacg
1261 tgaatgctcc tcccacattt cactctgaga tgatgtatga caatttcacc ctggtccctg
1321 tctggggaaa ggaaggctat ctccacgtct gttccaatgg cctctgctgt tatttacttt
1381 acgagaggcc caccttatcc aaagagctgt atgccctggg ggtctttgat gggcttcaca
1441 cagtacatgg cacttactac atccaagtgt gtgccctggt caggtgtggg ggtcttggct
1501 tcgacacctg tggacaggaa atcacagagg ccacggggat atttgagttt cacctgtggg
1561 gcaacttcag tacttcctat atctttcctt tgtttctgac ctcagggatg accctagaag
1621 tccctgacca gcttggttgg gagaatgacc actatttcct gaggaaaagt aggctgtcct
1681 ctgggctggt gacggcggct ctctatgggc gcttgtatga gagggactag gaaaagtgtg
1741 tggtctgtgg ggcggactct ggccatcatg ttgacagcct tgcacttcca caggctacaa
1801 gccctgggac catctttctg cctt
//