LOCUS AK297025 2924 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ55905 complete cds, highly similar to FYVE,
RhoGEF and PH domain-containing protein 4.
ACCESSION AK297025
VERSION AK297025.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2924)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2924
/clone="D3OST2003177"
/clone_lib="D3OST2"
/db_xref="H-InvDB:HIT000491649"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from CD34+ cells after 3-days ODF induction."
/note="primary culture, CD34+ cells"
/organism="Homo sapiens"
/tissue_type="cord blood"
CDS 163..2718
/codon_start=1
/note="highly similar to FYVE, RhoGEF and PH
domain-containing protein 4"
/protein_id="BAH12479.1"
/transl_table=1
/translation="MGGCSSTCPKIALVPPCSTSSTTTLVGENVSEEEAQGINGNRPA
KHSAASPKPQVPPKPLHLQNSPSSNIHQTPRHKALPSAKPRMEEIKPASASCVSKEKP
SKVSDLISRFEGGSSLSNYSDLKKESAVNLNAPRTPGRHGLTTTPQQKLLSQHLPQRQ
GNDTDKTQGAQTCVANGVMAAQNQMECEEEKAATLSSDTSIQASEPLLDTHIVNGERD
ETATAPASPTTDSCDGNASDSSYRTPGIGPVLPLEERGAETETKVQERENGESPLELE
QLDQHHEMKETNEQKLHKIANELLLTERAYVNRLDLLDQVFYCKLLEEANRGSFPAEM
VNKIFSNISSINAFHSKFLLPELEKRMQEWETTPRIGDILQKLAPFLKMYGEYVKGFD
NAMELVKNMTERIPQFKSVVEEIQKQKICGSLTLQHHMLEPVQRIPRYEMLLKDYLRK
LPPDSLDWNDAKKSLEIISTAASHSNSAIRKMENLKKLLEIYEMLGEEEDIVNPSNEL
IKEGQILKLAARNTSAQERYLFLFNNMLLYCVPKFSLVGSKFTVRTRVGIDGMKIVET
QNEEYPHTFQVSGKERTLELQASSAQDKEEWIKALQETIDAFHQRHETFRNAIAKDND
IHSEVSTAELGKRAPRWIRDNEVTMCMKCKEPFNALTRRRHHCRACGYVVCWKCSDYK
AQLEYDGGKLSKVCKDCYQIISGFTDSEEKKRKGILEIESAEVSGNSVVCSFLQYMEK
SKPWQKAWCVIPKQDPLVLYMYGAPQDVRAQATIPLLGYVVDEMPRSADLPHSFKLTQ
SKSVHSFAADSEELKQKWLKVILLAVTGETPGGPNEHPATLDDHPEPKKKSEC"
BASE COUNT 967 a 607 c 652 g 698 t
ORIGIN
1 agttagttct ttatcatgtt gaacactaga tgtgtatgtg tctagtgtct aaatacagaa
61 tgcctatagt ttctaccaac aaattatgga tccaaatcct tgctccagaa gcaggaaata
121 ggttaatcag aagattgctg gatgttatga aaagaatctt ttatgggggg ctgcagcagt
181 acctgtccaa agatcgcttt agttccacct tgctccacaa gcagcacaac cacactggtt
241 ggtgagaatg tatctgaaga agaggctcag ggaataaatg ggaacaggcc agcaaaacac
301 tcagctgcaa gtccaaagcc acaagtgcct ccaaagccat tacacctgca gaattcacct
361 tcgtccaata tacaccaaac ccccaggcat aaagctttac ctagtgcaaa accaaggatg
421 gaggaaatta aacctgcctc tgcttcttgt gtctcaaaag aaaaacccag taaggtatca
481 gatctcatca gtcgctttga aggaggcagc tcattatcaa attatagtga tttgaagaaa
541 gagtctgctg tgaacctaaa tgctcctaga accccaggaa ggcatggatt gacaaccaca
601 cctcaacaaa aactcctctc ccagcacttg ccacagaggc agggaaatga tacagataag
661 actcagggtg cacagacttg tgtggccaac ggtgtaatgg cagcacaaaa ccagatggaa
721 tgtgaggagg agaaagctgc cactcttagc tcagatactt ctattcaagc ttctgaaccc
781 ttgcttgata cgcacatagt gaatggagaa agagatgaaa ctgccacagc tcctgcatca
841 cccacaacag acagctgtga tggaaatgct tctgacagta gctacaggac tccaggcata
901 ggcccagtgc tccccctaga agaaagaggg gcagaaacag aaaccaaggt acaagagagg
961 gaaaatgggg aaagccctct ggaactggag cagctggacc agcaccatga gatgaaggag
1021 actaatgagc aaaaacttca caaaatagcc aatgaacttt tgcttactga aagagcttat
1081 gtcaaccgac ttgacctctt agatcaggta ttttattgca aactgttgga agaagcaaac
1141 cgaggctcgt ttccagcaga gatggtgaat aaaatctttt ctaatatttc atcaataaat
1201 gccttccata gtaaattcct cttgccagag ctggagaaac gaatgcaaga atgggaaact
1261 actcctagaa ttggagacat ccttcagaaa ttggcaccat tccttaagat gtatggagaa
1321 tatgtgaaag gatttgataa tgcaatggaa ttggttaaaa acatgacaga acgtattccc
1381 cagttcaaat cagtggttga agaaattcag aaacagaaaa tctgtgggag cttaactttg
1441 cagcatcaca tgctagaacc tgttcagcgg attccccggt atgagatgct ccttaaggac
1501 tatctaagga aattgcctcc tgattccctg gactggaatg atgctaaaaa atcacttgaa
1561 attatatcta cagcagcaag ccattctaat agtgcaataa ggaaaatgga gaacctaaag
1621 aaactcttag agatttatga aatgttggga gaagaagaag acattgtaaa cccttcaaat
1681 gaactaataa aagaaggaca gatcctcaaa ctagctgctc ggaacacttc agcacaagaa
1741 cgctaccttt tcttattcaa caacatgttg ctgtactgtg tgcccaaatt cagcttggta
1801 ggctctaaat tcacagttcg aaccagggtt ggcattgatg gaatgaaaat tgtagagact
1861 caaaatgaag aatatccaca tactttccag gtgtctggga aagagagaac actggaactg
1921 caggccagtt ctgcgcaaga caaagaagaa tggatcaagg cccttcaaga aaccatcgat
1981 gcttttcatc aaaggcatga aaccttcaga aatgcaattg caaaggataa tgacattcac
2041 tcagaggttt ctactgctga gctagggaaa agagccccaa gatggatccg agataatgaa
2101 gtgacaatgt gtatgaaatg taaagaacct ttcaatgcac tgacacgaag gaggcatcat
2161 tgtcgagcat gtggatatgt ggtttgttgg aaatgctccg actacaaagc tcaacttgaa
2221 tatgatggtg gtaaattgag caaagtttgt aaagactgtt atcaaatcat aagtggattc
2281 acagacagtg aagaaaagaa aagaaaagga attttagaga ttgaatcagc agaagtatct
2341 ggaaacagtg tggtgtgcag ctttcttcag tatatggaga agtcaaaacc ttggcagaaa
2401 gcttggtgtg tgatccccaa gcaagaccct cttgtgctgt acatgtatgg tgccccccag
2461 gacgtcagag cccaggccac cattccactt ctgggctatg tggtggatga aatgccaagg
2521 agcgcagacc tgccacacag tttcaaactg acccagtcta agtccgtgca cagctttgct
2581 gcagacagtg aggaactgaa gcagaagtgg ctgaaagtca tccttttagc tgtcacaggt
2641 gagacaccag gtggtccaaa tgagcatcca gccaccttgg atgatcatcc tgaacctaag
2701 aaaaaatcag aatgctgaac tcctccagga ccagccatgg tgtggaggtc tcaggactta
2761 cagctcaaga cattcccagc tcttcttaca catctgctag cactttatgt tgaaaaatat
2821 aggcccataa atgcatcttt tgaggactat tttcctatgt ttatgtactc ttagtgaaat
2881 tagtgtgcag agtcattcta ccgataaagt tttgaaataa tgtg
//