LOCUS AK297021 1774 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ50569 complete cds, highly similar to
Myotubularin (EC 3.1.3.48).
ACCESSION AK297021
VERSION AK297021.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1774)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1774
/clone="D3OST2002509"
/clone_lib="D3OST2"
/db_xref="H-InvDB:HIT000491645"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from CD34+ cells after 3-days ODF induction."
/note="primary culture, CD34+ cells"
/organism="Homo sapiens"
/tissue_type="cord blood"
CDS 31..1731
/codon_start=1
/note="highly similar to Myotubularin (EC 3.1.3.48)"
/protein_id="BAH12477.1"
/transl_table=1
/translation="MASASTSKYNSHSLENESIKRTSRDGVNRDLTEAVPRLPGETLI
TDKEVIYICPFNGPIKGRVYITNYRLYLRSLETDMRNLRFALKQEGHSRRDMFEILTR
YAFPLAHSLPLFAFLNEEKFNVDGWTVYNPVEEYRRQGLPNHHWRITFINKCYELCDT
YPALLVVPYRASDDDLRRVATFRSRNRIPVLSWIHPENKTVIVRCSQPLVGMSGKRNK
DDEKYLDVIRETNKQISKLTIYDARPSVNAVANKATGGGYESDDAYHNAELFFLDIHN
IHVMRESLKKVKDIVYPNVEESHWLSSLESTHWLEHIKLVLTGAIQVADKVSSGKSSV
LVHCSDGWDRTAQLTSLAMLMLDSFYRSIEGFEILVQKEWISFGHKFASRIGHGDKNH
TDADRSPIFLQFIDCVWQMSKQFPTAFEFNEQFLIIILDHLYSCRFGTFLFNCESARE
RQKVTERTVSLWSLINSNKEKFKNPFYTKEINRVLYPVASMRHLELWVNYYIRWNPRI
KQQQPNPVEQRYMELLALRDEYIKRLEELQLANSAKLSDPPTSPSSPSQMMPHVQTHF
"
BASE COUNT 531 a 356 c 394 g 493 t
ORIGIN
1 ggtggcgccc ggagcccgag agtttccagg atggcttctg catcaacttc taaatataat
61 tcacactcct tggagaatga gtctattaag aggacgtctc gagatggagt caatcgagat
121 ctcactgagg ctgttcctcg acttccagga gaaacactaa tcactgacaa agaagttatt
181 tacatatgtc ctttcaatgg ccccattaag ggaagagttt acatcacaaa ttatcgtctt
241 tatttaagaa gtttggaaac ggacatgaga aacctgaggt tcgctttgaa acaggaaggc
301 cacagcagaa gagatatgtt tgagatcctc acgagatacg cgtttcccct ggctcacagt
361 ctgccattat ttgcattttt aaatgaagaa aagtttaacg tggatggatg gacagtttac
421 aatccagtgg aagaatacag gaggcagggc ttgcccaatc accattggag aataactttt
481 attaataagt gctatgagct ctgtgacact taccctgctc ttttggtggt tccgtatcgt
541 gcctcagatg atgacctccg gagagttgca acttttaggt cccgaaatcg aattccagtg
601 ctgtcatgga ttcatccaga aaataagacg gtcattgtgc gttgcagtca gcctcttgtc
661 ggtatgagtg ggaaacgaaa taaagatgat gagaaatatc tcgatgttat cagggagact
721 aataaacaaa tttctaaact caccatttat gatgcaagac ccagcgtaaa tgcagtggcc
781 aacaaggcaa caggaggagg atatgaaagt gatgatgcat atcataacgc cgaacttttc
841 ttcttagaca ttcataatat tcatgttatg cgggaatctt taaaaaaagt gaaggacatt
901 gtttatccta atgtagaaga atctcattgg ttgtccagtt tggagtctac tcattggtta
961 gaacatatca agctcgtttt gacaggagcc attcaagtag cagacaaagt ttcttcaggg
1021 aagagttcag tgcttgtgca ttgcagtgac ggatgggaca ggactgctca gctgacatcc
1081 ttggccatgc tgatgttgga tagcttctat aggagcattg aagggttcga aatactggta
1141 caaaaagaat ggataagttt tggacataaa tttgcatctc gaataggtca tggtgataaa
1201 aaccacaccg atgctgaccg ttctcctatt tttctccagt ttattgattg tgtgtggcaa
1261 atgtcaaaac agttccctac agcttttgaa ttcaatgaac aatttttgat tataattttg
1321 gatcatctgt atagttgccg atttggtact ttcttattca actgtgaatc tgctcgagaa
1381 agacagaagg ttacagaaag gactgtttct ttatggtcac tgataaacag taataaagaa
1441 aaattcaaaa accccttcta tactaaagaa atcaatcgag ttttatatcc agttgccagt
1501 atgcgtcact tggaactctg ggtgaattac tacattagat ggaaccccag gatcaagcaa
1561 caacagccga atccagtgga gcagcgttac atggagctct tagccttacg cgacgaatac
1621 ataaagcggc ttgaggaact gcagctcgcc aactctgcca agctttctga tcccccaact
1681 tcaccttcca gtccttcgca aatgatgccc catgtgcaaa ctcacttctg aggggggacc
1741 ctggcaccgc attagagctc gaaataaagg cgat
//